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Highly polygenic variation in environmental perception determines dauer larvae formation in growing populations of Caenorhabditis elegans.

Green JW, Stastna JJ, Orbidans HE, Harvey SC - PLoS ONE (2014)

Bottom Line: We also show that food patch size affects both the ability to detect QTLs and estimates of effect size, and demonstrate that an allele of nath-10 affects dauer larvae formation in growing populations.These data indicate that QTLs affecting the number of dauer larvae at food exhaustion in growing populations of C. elegans are highly reproducible, and that nearly all can be explained by variation affecting dauer larvae formation in response to defined amounts of pheromone.This suggests that most variation in dauer larvae formation in growing populations is a consequence of variation in the perception of the food and pheromone environment (i.e. chemosensory variation) and in the integration of these cues.

View Article: PubMed Central - PubMed

Affiliation: Biomolecular Research Group, School of Human and Life Sciences, Canterbury Christ Church University, Canterbury, United Kingdom.

ABSTRACT

Background: Determining how complex traits are genetically controlled is a requirement if we are to predict how they evolve and how they might respond to selection. This requires understanding how distinct, and often more simple, life history traits interact and change in response to environmental conditions. In order to begin addressing such issues, we have been analyzing the formation of the developmentally arrested dauer larvae of Caenorhabditis elegans under different conditions.

Results: We find that 18 of 22 previously identified quantitative trait loci (QTLs) affecting dauer larvae formation in growing populations, assayed by determining the number of dauer larvae present at food patch exhaustion, can be recovered under various environmental conditions. We also show that food patch size affects both the ability to detect QTLs and estimates of effect size, and demonstrate that an allele of nath-10 affects dauer larvae formation in growing populations. To investigate the component traits that affect dauer larvae formation in growing populations we map, using the same introgression lines, QTLs that affect dauer larvae formation in response to defined amounts of pheromone. This identifies 36 QTLs, again demonstrating the highly polygenic nature of the genetic variation underlying dauer larvae formation.

Conclusions: These data indicate that QTLs affecting the number of dauer larvae at food exhaustion in growing populations of C. elegans are highly reproducible, and that nearly all can be explained by variation affecting dauer larvae formation in response to defined amounts of pheromone. This suggests that most variation in dauer larvae formation in growing populations is a consequence of variation in the perception of the food and pheromone environment (i.e. chemosensory variation) and in the integration of these cues.

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Related in: MedlinePlus

Most QTLs affecting the number of dauer larvae at food exhaustion in growing populations overlap with QTLs affecting dauer larvae formation in response to defined amounts of pheromone.Venn diagrams showing the overlap between QTLs that affect dauer larvae development in response to defined amounts of pheromone (upper circles) and QTLs that affect the number of dauer larvae at food exhaustion in growing populations (lower circles). Negative effect QTLs, where the CB4856 allele decreases the number of dauer larvae, are shown in the left hand diagram and positive effect QTLs, where the CB4856 allele increases the number of dauer larvae, are shown in the right hand diagram.
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pone-0112830-g006: Most QTLs affecting the number of dauer larvae at food exhaustion in growing populations overlap with QTLs affecting dauer larvae formation in response to defined amounts of pheromone.Venn diagrams showing the overlap between QTLs that affect dauer larvae development in response to defined amounts of pheromone (upper circles) and QTLs that affect the number of dauer larvae at food exhaustion in growing populations (lower circles). Negative effect QTLs, where the CB4856 allele decreases the number of dauer larvae, are shown in the left hand diagram and positive effect QTLs, where the CB4856 allele increases the number of dauer larvae, are shown in the right hand diagram.

Mentions: Comparison of the QTLs detected in growing populations (Table 1, [28]) with those affecting dauer larvae formation in response to defined amounts of pheromone (Figure 3, Table 2) indicates that the majority of QTLs affecting the number of dauer larvae in growing populations co-localize with those that affect dauer larvae formation in response to defined amounts of pheromone (Table 2, Figure 6). Of the QTLs that only affect the number of dauer larvae at food exhaustion in growing populations, gp3 is not supported in this analysis (Table 1), and another, gp21, is at least in part due to variation at npr-1[28], a polymorphism that does not affect the likelihood of dauer larvae formation in standard dauer assays [22].


Highly polygenic variation in environmental perception determines dauer larvae formation in growing populations of Caenorhabditis elegans.

Green JW, Stastna JJ, Orbidans HE, Harvey SC - PLoS ONE (2014)

Most QTLs affecting the number of dauer larvae at food exhaustion in growing populations overlap with QTLs affecting dauer larvae formation in response to defined amounts of pheromone.Venn diagrams showing the overlap between QTLs that affect dauer larvae development in response to defined amounts of pheromone (upper circles) and QTLs that affect the number of dauer larvae at food exhaustion in growing populations (lower circles). Negative effect QTLs, where the CB4856 allele decreases the number of dauer larvae, are shown in the left hand diagram and positive effect QTLs, where the CB4856 allele increases the number of dauer larvae, are shown in the right hand diagram.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231163&req=5

pone-0112830-g006: Most QTLs affecting the number of dauer larvae at food exhaustion in growing populations overlap with QTLs affecting dauer larvae formation in response to defined amounts of pheromone.Venn diagrams showing the overlap between QTLs that affect dauer larvae development in response to defined amounts of pheromone (upper circles) and QTLs that affect the number of dauer larvae at food exhaustion in growing populations (lower circles). Negative effect QTLs, where the CB4856 allele decreases the number of dauer larvae, are shown in the left hand diagram and positive effect QTLs, where the CB4856 allele increases the number of dauer larvae, are shown in the right hand diagram.
Mentions: Comparison of the QTLs detected in growing populations (Table 1, [28]) with those affecting dauer larvae formation in response to defined amounts of pheromone (Figure 3, Table 2) indicates that the majority of QTLs affecting the number of dauer larvae in growing populations co-localize with those that affect dauer larvae formation in response to defined amounts of pheromone (Table 2, Figure 6). Of the QTLs that only affect the number of dauer larvae at food exhaustion in growing populations, gp3 is not supported in this analysis (Table 1), and another, gp21, is at least in part due to variation at npr-1[28], a polymorphism that does not affect the likelihood of dauer larvae formation in standard dauer assays [22].

Bottom Line: We also show that food patch size affects both the ability to detect QTLs and estimates of effect size, and demonstrate that an allele of nath-10 affects dauer larvae formation in growing populations.These data indicate that QTLs affecting the number of dauer larvae at food exhaustion in growing populations of C. elegans are highly reproducible, and that nearly all can be explained by variation affecting dauer larvae formation in response to defined amounts of pheromone.This suggests that most variation in dauer larvae formation in growing populations is a consequence of variation in the perception of the food and pheromone environment (i.e. chemosensory variation) and in the integration of these cues.

View Article: PubMed Central - PubMed

Affiliation: Biomolecular Research Group, School of Human and Life Sciences, Canterbury Christ Church University, Canterbury, United Kingdom.

ABSTRACT

Background: Determining how complex traits are genetically controlled is a requirement if we are to predict how they evolve and how they might respond to selection. This requires understanding how distinct, and often more simple, life history traits interact and change in response to environmental conditions. In order to begin addressing such issues, we have been analyzing the formation of the developmentally arrested dauer larvae of Caenorhabditis elegans under different conditions.

Results: We find that 18 of 22 previously identified quantitative trait loci (QTLs) affecting dauer larvae formation in growing populations, assayed by determining the number of dauer larvae present at food patch exhaustion, can be recovered under various environmental conditions. We also show that food patch size affects both the ability to detect QTLs and estimates of effect size, and demonstrate that an allele of nath-10 affects dauer larvae formation in growing populations. To investigate the component traits that affect dauer larvae formation in growing populations we map, using the same introgression lines, QTLs that affect dauer larvae formation in response to defined amounts of pheromone. This identifies 36 QTLs, again demonstrating the highly polygenic nature of the genetic variation underlying dauer larvae formation.

Conclusions: These data indicate that QTLs affecting the number of dauer larvae at food exhaustion in growing populations of C. elegans are highly reproducible, and that nearly all can be explained by variation affecting dauer larvae formation in response to defined amounts of pheromone. This suggests that most variation in dauer larvae formation in growing populations is a consequence of variation in the perception of the food and pheromone environment (i.e. chemosensory variation) and in the integration of these cues.

Show MeSH
Related in: MedlinePlus