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Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.

Noulin F, Manesia JK, Rosanas-Urgell A, Erhart A, Borlon C, Van Den Abbeele J, d'Alessandro U, Verfaillie CM - PLoS ONE (2014)

Bottom Line: We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB).Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax.As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.

View Article: PubMed Central - PubMed

Affiliation: Unit of Malariology, Institute of Tropical Medicine, Antwerp, Belgium.

ABSTRACT
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.

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Cytopsin of the P. vivax culture 24 h post-invasion for different sources of reticulocytes.A) UCB/HSPC, B) BM/HSPC, C) PB/HSPC, D) reticulocytes-enriched UCB and E) reticulocytes-enriched PB. The left panels represent pictures with a 63× magnification and the corresponding right panels represent a 100× of the left picture square. P. vivax infected cells are under arrow.
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pone-0112496-g003: Cytopsin of the P. vivax culture 24 h post-invasion for different sources of reticulocytes.A) UCB/HSPC, B) BM/HSPC, C) PB/HSPC, D) reticulocytes-enriched UCB and E) reticulocytes-enriched PB. The left panels represent pictures with a 63× magnification and the corresponding right panels represent a 100× of the left picture square. P. vivax infected cells are under arrow.

Mentions: P. vivax parasites invaded reticulocytes derived from CD34+ cells or directly obtained from enriched blood (PB or UCB; Figure 3). The mean invasion rates for the different sources of HSPC sources were 3.05%, 3.05% and 3.15% respectively for UCB, BM and PB (n = 4). The means for UCB-concentrated reticulocytes (n = 4) and PB-concentrated reticulocytes (n = 3) were respectively 1.4% and 0.2%.


Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.

Noulin F, Manesia JK, Rosanas-Urgell A, Erhart A, Borlon C, Van Den Abbeele J, d'Alessandro U, Verfaillie CM - PLoS ONE (2014)

Cytopsin of the P. vivax culture 24 h post-invasion for different sources of reticulocytes.A) UCB/HSPC, B) BM/HSPC, C) PB/HSPC, D) reticulocytes-enriched UCB and E) reticulocytes-enriched PB. The left panels represent pictures with a 63× magnification and the corresponding right panels represent a 100× of the left picture square. P. vivax infected cells are under arrow.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4231068&req=5

pone-0112496-g003: Cytopsin of the P. vivax culture 24 h post-invasion for different sources of reticulocytes.A) UCB/HSPC, B) BM/HSPC, C) PB/HSPC, D) reticulocytes-enriched UCB and E) reticulocytes-enriched PB. The left panels represent pictures with a 63× magnification and the corresponding right panels represent a 100× of the left picture square. P. vivax infected cells are under arrow.
Mentions: P. vivax parasites invaded reticulocytes derived from CD34+ cells or directly obtained from enriched blood (PB or UCB; Figure 3). The mean invasion rates for the different sources of HSPC sources were 3.05%, 3.05% and 3.15% respectively for UCB, BM and PB (n = 4). The means for UCB-concentrated reticulocytes (n = 4) and PB-concentrated reticulocytes (n = 3) were respectively 1.4% and 0.2%.

Bottom Line: We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB).Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax.As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.

View Article: PubMed Central - PubMed

Affiliation: Unit of Malariology, Institute of Tropical Medicine, Antwerp, Belgium.

ABSTRACT
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.

Show MeSH
Related in: MedlinePlus