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Elevated soluble CD163 plasma levels are associated with disease severity in patients with hemorrhagic fever with renal syndrome.

Wang J, Guo W, Du H, Yu H, Jiang W, Zhu T, Bai X, Wang P - PLoS ONE (2014)

Bottom Line: The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed.The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001).The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001).

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.

ABSTRACT

Background: Hantaan virus is a major zoonotic pathogen that causesing hemorrhagic fever with renal syndrome (HFRS). Although HFRS pathogenesis has not been entirely elucidated, the importance of host-related immune responses in HFRS pathogenesis has been widely recognized. CD163, a monocyte and macrophage-specific scavenger receptor that plays a vital function in the hosts can reduce inflammation, is shed during activation as soluble CD163 (sCD163). The aim of this study was to investigate the pathological significance of sCD163 in patients with HFRS.

Methods: Blood samples were collected from 81 hospitalized patients in Tangdu Hospital from October 2011 to January 2014 and from 15 healthy controls. The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed. Furthermore, CD163 expression in 3 monocytes subset was analyzed by flow cytometry.

Results: The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001). The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001). A Spearman correlation analysis indicated that the sCD163 levels were positively correlated with white blood cell, serum creatine, blood urea nitrogen levels, while they were negatively correlated with blood platelet levels in the HFRS patients. The monocyte subsets were significantly altered during the acute stage. Though the CD163 expression levels within the monocyte subsets were increased during the acute stage, the highest CD163 expression level was observed in the CD14++CD16+ monocytes when compared with the other monocyte subsets.

Conclusion: sCD163 may be correlated with disease severity and the disease progression in HFRS patients; however, the underlying mechanisms should be explored further.

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Related in: MedlinePlus

CD163 was differentially expressed on the monocyte subsets.(A) CD163 protein expression (black lines) in each monocyte subset is shown compared with the matched isotype control (shaded histograms) in the histograms. The data illustrate results from a representative experiment. (B) The CD163 expression summary data for the three monocyte subsets during the acute and convalescent phases and in the normal controls. The significance of the differences among the multiple groups was determined with the Kruskal-Wallis test. The black lines represent medians and P values are plotted for each graph.
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pone-0112127-g004: CD163 was differentially expressed on the monocyte subsets.(A) CD163 protein expression (black lines) in each monocyte subset is shown compared with the matched isotype control (shaded histograms) in the histograms. The data illustrate results from a representative experiment. (B) The CD163 expression summary data for the three monocyte subsets during the acute and convalescent phases and in the normal controls. The significance of the differences among the multiple groups was determined with the Kruskal-Wallis test. The black lines represent medians and P values are plotted for each graph.

Mentions: As is known, the CD163 expression on the monocyte subsets surface is different [15]. In this study, we confirmed that the CD163 expression on the CD14++CD16+ monocyte subset was most highly expressed compared with less expression on the CD14++CD16− monocyte subset and lower levels on the CD14+CD16++ monocyte subset (Figure 4B). CD163 expression on the CD14++CD16+ monocyte was more remarkable during the acute phase (median = 64.2%, IQR = 38.8%–88.5%, P<0.0001) than during the convalescent phase (median = 17.3%, IQR = 11.3%–24.5%) in the HFRS patients and healthy controls (median = 16.3%, IQR = 9.4%–21.3%); however no significant (P>0.05) differences were observed during the convalescent phase and in the healthy controls. CD163 expression on the classical and non-classical monocytes were increased during the acute phase (median = 43.6%, IQR = 25.9%–68.8% and median = 30.0%, IQR = 22.4%–48.6%, P<0.0001) when compared with the convalescent phase (median = 14.0%, IQR = 8.1%–16.5% and median = 12.3%, IQR = 9.1%–21.1%) and healthy control findings (median = 11.6%, IQR = 8.3%–16.2% and median = 9.6%, IQR = 6.7%–15.4%); however, no significant differences (P>0.05) were observed in the convalescent phase and healthy control samples, (Figure 4B).


Elevated soluble CD163 plasma levels are associated with disease severity in patients with hemorrhagic fever with renal syndrome.

Wang J, Guo W, Du H, Yu H, Jiang W, Zhu T, Bai X, Wang P - PLoS ONE (2014)

CD163 was differentially expressed on the monocyte subsets.(A) CD163 protein expression (black lines) in each monocyte subset is shown compared with the matched isotype control (shaded histograms) in the histograms. The data illustrate results from a representative experiment. (B) The CD163 expression summary data for the three monocyte subsets during the acute and convalescent phases and in the normal controls. The significance of the differences among the multiple groups was determined with the Kruskal-Wallis test. The black lines represent medians and P values are plotted for each graph.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230986&req=5

pone-0112127-g004: CD163 was differentially expressed on the monocyte subsets.(A) CD163 protein expression (black lines) in each monocyte subset is shown compared with the matched isotype control (shaded histograms) in the histograms. The data illustrate results from a representative experiment. (B) The CD163 expression summary data for the three monocyte subsets during the acute and convalescent phases and in the normal controls. The significance of the differences among the multiple groups was determined with the Kruskal-Wallis test. The black lines represent medians and P values are plotted for each graph.
Mentions: As is known, the CD163 expression on the monocyte subsets surface is different [15]. In this study, we confirmed that the CD163 expression on the CD14++CD16+ monocyte subset was most highly expressed compared with less expression on the CD14++CD16− monocyte subset and lower levels on the CD14+CD16++ monocyte subset (Figure 4B). CD163 expression on the CD14++CD16+ monocyte was more remarkable during the acute phase (median = 64.2%, IQR = 38.8%–88.5%, P<0.0001) than during the convalescent phase (median = 17.3%, IQR = 11.3%–24.5%) in the HFRS patients and healthy controls (median = 16.3%, IQR = 9.4%–21.3%); however no significant (P>0.05) differences were observed during the convalescent phase and in the healthy controls. CD163 expression on the classical and non-classical monocytes were increased during the acute phase (median = 43.6%, IQR = 25.9%–68.8% and median = 30.0%, IQR = 22.4%–48.6%, P<0.0001) when compared with the convalescent phase (median = 14.0%, IQR = 8.1%–16.5% and median = 12.3%, IQR = 9.1%–21.1%) and healthy control findings (median = 11.6%, IQR = 8.3%–16.2% and median = 9.6%, IQR = 6.7%–15.4%); however, no significant differences (P>0.05) were observed in the convalescent phase and healthy control samples, (Figure 4B).

Bottom Line: The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed.The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001).The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001).

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.

ABSTRACT

Background: Hantaan virus is a major zoonotic pathogen that causesing hemorrhagic fever with renal syndrome (HFRS). Although HFRS pathogenesis has not been entirely elucidated, the importance of host-related immune responses in HFRS pathogenesis has been widely recognized. CD163, a monocyte and macrophage-specific scavenger receptor that plays a vital function in the hosts can reduce inflammation, is shed during activation as soluble CD163 (sCD163). The aim of this study was to investigate the pathological significance of sCD163 in patients with HFRS.

Methods: Blood samples were collected from 81 hospitalized patients in Tangdu Hospital from October 2011 to January 2014 and from 15 healthy controls. The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed. Furthermore, CD163 expression in 3 monocytes subset was analyzed by flow cytometry.

Results: The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001). The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001). A Spearman correlation analysis indicated that the sCD163 levels were positively correlated with white blood cell, serum creatine, blood urea nitrogen levels, while they were negatively correlated with blood platelet levels in the HFRS patients. The monocyte subsets were significantly altered during the acute stage. Though the CD163 expression levels within the monocyte subsets were increased during the acute stage, the highest CD163 expression level was observed in the CD14++CD16+ monocytes when compared with the other monocyte subsets.

Conclusion: sCD163 may be correlated with disease severity and the disease progression in HFRS patients; however, the underlying mechanisms should be explored further.

Show MeSH
Related in: MedlinePlus