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Elevated soluble CD163 plasma levels are associated with disease severity in patients with hemorrhagic fever with renal syndrome.

Wang J, Guo W, Du H, Yu H, Jiang W, Zhu T, Bai X, Wang P - PLoS ONE (2014)

Bottom Line: The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed.The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001).The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001).

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.

ABSTRACT

Background: Hantaan virus is a major zoonotic pathogen that causesing hemorrhagic fever with renal syndrome (HFRS). Although HFRS pathogenesis has not been entirely elucidated, the importance of host-related immune responses in HFRS pathogenesis has been widely recognized. CD163, a monocyte and macrophage-specific scavenger receptor that plays a vital function in the hosts can reduce inflammation, is shed during activation as soluble CD163 (sCD163). The aim of this study was to investigate the pathological significance of sCD163 in patients with HFRS.

Methods: Blood samples were collected from 81 hospitalized patients in Tangdu Hospital from October 2011 to January 2014 and from 15 healthy controls. The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed. Furthermore, CD163 expression in 3 monocytes subset was analyzed by flow cytometry.

Results: The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001). The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001). A Spearman correlation analysis indicated that the sCD163 levels were positively correlated with white blood cell, serum creatine, blood urea nitrogen levels, while they were negatively correlated with blood platelet levels in the HFRS patients. The monocyte subsets were significantly altered during the acute stage. Though the CD163 expression levels within the monocyte subsets were increased during the acute stage, the highest CD163 expression level was observed in the CD14++CD16+ monocytes when compared with the other monocyte subsets.

Conclusion: sCD163 may be correlated with disease severity and the disease progression in HFRS patients; however, the underlying mechanisms should be explored further.

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Related in: MedlinePlus

The monocyte subset proportions were altered in the HFRS subjects.A representative example of the monocyte subset analysis procedure is displayed (A, B, and C). (A) Monocytes from the total PBMC population were identified by forward (FSC) and side scatter (SSC) property analysis. (B) The isotypic negative control staining of CD14 and CD16 is displayed. (C) Using surface CD14 and CD16 expression, monocyte gated populations were further divided into three monocyte subsets, which were classic (CD14++CD16−), intermediate (CD14++CD16+) and non-classical monocytes (CD14+CD16++). A summary of the monocyte subset distribution analyses during the acute and convalescent HFRS phases and in the healthy subjects is displayed (D, E, and F). The significance of the differences among the multiple groups was determined by the Kruskal-Wallis test, Black lines represent medians and P values are plotted in each graph.
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pone-0112127-g003: The monocyte subset proportions were altered in the HFRS subjects.A representative example of the monocyte subset analysis procedure is displayed (A, B, and C). (A) Monocytes from the total PBMC population were identified by forward (FSC) and side scatter (SSC) property analysis. (B) The isotypic negative control staining of CD14 and CD16 is displayed. (C) Using surface CD14 and CD16 expression, monocyte gated populations were further divided into three monocyte subsets, which were classic (CD14++CD16−), intermediate (CD14++CD16+) and non-classical monocytes (CD14+CD16++). A summary of the monocyte subset distribution analyses during the acute and convalescent HFRS phases and in the healthy subjects is displayed (D, E, and F). The significance of the differences among the multiple groups was determined by the Kruskal-Wallis test, Black lines represent medians and P values are plotted in each graph.

Mentions: Monocytes and their subsets were identified by flow cytometry based on their forward and side scatter characteristics and by their CD14 and CD16 expression levels [27]. The gating strategy used to identify the classical (CD14++CD16−), intermediate (CD14++CD16+), and non-classical monocytes (CD14+CD16++) is shown in Figure 3C. Additionally, summary figures are displayed in Figures 3D, 3E, and 3F. The intermediate monocyte proportions (CD14++CD16+) were significantly increased during the patients of acute phase (median = 18.5%, interquartile range IQR = 15.3%–24.9%, P<0.0001) compared with the patients of convalescent phase (median = 6.5%, IQR = 5.1%–8.5%) and the healthy control (median = 5.0%, IQR = 3.3%–7.1%). However, no significant differences (P> 0.05) were observed between the convalescent phase and healthy control proportions, (Figures 3E). The classical (CD14++CD16−) and non-classical (CD14+CD16++) monocyte proportions were both significantly decreased during the acute phase (median = 68.4%, IQR = 61.8%–75.1%, and median = 1.6%, IQR = 1.1%–2.58%, P<0.0001) compared with those during the convalescent phase (median = 80.0%, IQR = 76.5%–81.6% and median = 3.6%, IQR = 2.5%–5.5%) and in the healthy controls (median = 83.0%, IQR = 80.1%–84.7% and median = 4.5%, IQR = 3.2%–6.1%); however no significant differences between both monocyte subsets (P>0.05) were observed between the patients of convalescent phase and the healthy controls, (Figures 3D and 3F).


Elevated soluble CD163 plasma levels are associated with disease severity in patients with hemorrhagic fever with renal syndrome.

Wang J, Guo W, Du H, Yu H, Jiang W, Zhu T, Bai X, Wang P - PLoS ONE (2014)

The monocyte subset proportions were altered in the HFRS subjects.A representative example of the monocyte subset analysis procedure is displayed (A, B, and C). (A) Monocytes from the total PBMC population were identified by forward (FSC) and side scatter (SSC) property analysis. (B) The isotypic negative control staining of CD14 and CD16 is displayed. (C) Using surface CD14 and CD16 expression, monocyte gated populations were further divided into three monocyte subsets, which were classic (CD14++CD16−), intermediate (CD14++CD16+) and non-classical monocytes (CD14+CD16++). A summary of the monocyte subset distribution analyses during the acute and convalescent HFRS phases and in the healthy subjects is displayed (D, E, and F). The significance of the differences among the multiple groups was determined by the Kruskal-Wallis test, Black lines represent medians and P values are plotted in each graph.
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Related In: Results  -  Collection

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pone-0112127-g003: The monocyte subset proportions were altered in the HFRS subjects.A representative example of the monocyte subset analysis procedure is displayed (A, B, and C). (A) Monocytes from the total PBMC population were identified by forward (FSC) and side scatter (SSC) property analysis. (B) The isotypic negative control staining of CD14 and CD16 is displayed. (C) Using surface CD14 and CD16 expression, monocyte gated populations were further divided into three monocyte subsets, which were classic (CD14++CD16−), intermediate (CD14++CD16+) and non-classical monocytes (CD14+CD16++). A summary of the monocyte subset distribution analyses during the acute and convalescent HFRS phases and in the healthy subjects is displayed (D, E, and F). The significance of the differences among the multiple groups was determined by the Kruskal-Wallis test, Black lines represent medians and P values are plotted in each graph.
Mentions: Monocytes and their subsets were identified by flow cytometry based on their forward and side scatter characteristics and by their CD14 and CD16 expression levels [27]. The gating strategy used to identify the classical (CD14++CD16−), intermediate (CD14++CD16+), and non-classical monocytes (CD14+CD16++) is shown in Figure 3C. Additionally, summary figures are displayed in Figures 3D, 3E, and 3F. The intermediate monocyte proportions (CD14++CD16+) were significantly increased during the patients of acute phase (median = 18.5%, interquartile range IQR = 15.3%–24.9%, P<0.0001) compared with the patients of convalescent phase (median = 6.5%, IQR = 5.1%–8.5%) and the healthy control (median = 5.0%, IQR = 3.3%–7.1%). However, no significant differences (P> 0.05) were observed between the convalescent phase and healthy control proportions, (Figures 3E). The classical (CD14++CD16−) and non-classical (CD14+CD16++) monocyte proportions were both significantly decreased during the acute phase (median = 68.4%, IQR = 61.8%–75.1%, and median = 1.6%, IQR = 1.1%–2.58%, P<0.0001) compared with those during the convalescent phase (median = 80.0%, IQR = 76.5%–81.6% and median = 3.6%, IQR = 2.5%–5.5%) and in the healthy controls (median = 83.0%, IQR = 80.1%–84.7% and median = 4.5%, IQR = 3.2%–6.1%); however no significant differences between both monocyte subsets (P>0.05) were observed between the patients of convalescent phase and the healthy controls, (Figures 3D and 3F).

Bottom Line: The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed.The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001).The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001).

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.

ABSTRACT

Background: Hantaan virus is a major zoonotic pathogen that causesing hemorrhagic fever with renal syndrome (HFRS). Although HFRS pathogenesis has not been entirely elucidated, the importance of host-related immune responses in HFRS pathogenesis has been widely recognized. CD163, a monocyte and macrophage-specific scavenger receptor that plays a vital function in the hosts can reduce inflammation, is shed during activation as soluble CD163 (sCD163). The aim of this study was to investigate the pathological significance of sCD163 in patients with HFRS.

Methods: Blood samples were collected from 81 hospitalized patients in Tangdu Hospital from October 2011 to January 2014 and from 15 healthy controls. The sCD163 plasma levels were measured using a sandwich ELISA, and the relationship between sCD163 and disease severity was analyzed. Furthermore, CD163 expression in 3 monocytes subset was analyzed by flow cytometry.

Results: The results demonstrated that sCD163 plasma levels during the HFRS acute phase were significantly higher in patients than during the convalescent stage and the levels in the healthy controls (P<0.0001). The sCD163 plasma levels in the severe/critical group were higher than those in the mild/moderate group during the acute (P<0.0001). A Spearman correlation analysis indicated that the sCD163 levels were positively correlated with white blood cell, serum creatine, blood urea nitrogen levels, while they were negatively correlated with blood platelet levels in the HFRS patients. The monocyte subsets were significantly altered during the acute stage. Though the CD163 expression levels within the monocyte subsets were increased during the acute stage, the highest CD163 expression level was observed in the CD14++CD16+ monocytes when compared with the other monocyte subsets.

Conclusion: sCD163 may be correlated with disease severity and the disease progression in HFRS patients; however, the underlying mechanisms should be explored further.

Show MeSH
Related in: MedlinePlus