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Coincident pre-diabetes is associated with dysregulated cytokine responses in pulmonary tuberculosis.

Kumar NP, Banurekha VV, Nair D, Sridhar R, Kornfeld H, Nutman TB, Babu S - PLoS ONE (2014)

Bottom Line: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines.However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

View Article: PubMed Central - PubMed

Affiliation: National Institutes of Health-International Center for Excellence in Research, Chennai, India; National Institute for Research in Tuberculosis, Chennai, India.

ABSTRACT

Background: Cytokines play an important role in the pathogenesis of pulmonary tuberculosis (PTB)--Type 2 diabetes mellitus co-morbidity. However, the cytokine interactions that characterize PTB coincident with pre-diabetes (PDM) are not known.

Methods: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).

Results: TB-PDM is characterized by elevated circulating levels of Type 1 (IFNγ, TNFα and IL-2), Type 17 (IL-17A and IL-17F) and other pro-inflammatory (IL-1β, IFNβ and GM-CSF) cytokines. TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines. Moreover, TB antigen stimulated whole blood also showed increased levels of pro-inflammatory (IFNγ, TNFα and IL-1β) cytokines as well. However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

Conclusion: Our data reveal that pre-diabetes in PTB individuals is characterized by heightened cytokine responsiveness, indicating that a balanced pro and anti - inflammatory cytokine milieu is a feature of pre-diabetes--TB co-morbidity.

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No relationship between systemic levels of cytokines and bacterial burdens in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-10, IL-1β, IFNβ, TGFβ and bacterial burdens (as assessed by smear grades - 1+, 2+ and 3+) was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.
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pone-0112108-g004: No relationship between systemic levels of cytokines and bacterial burdens in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-10, IL-1β, IFNβ, TGFβ and bacterial burdens (as assessed by smear grades - 1+, 2+ and 3+) was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.

Mentions: Since smear grades are an accurate reflection of bacterial burdens, we also sought to examine the relationship between the systemic levels of Type 1, Type 17 and other pro-inflammatory cytokines with the bacterial burdens in the TB-PDM individuals. As shown in Figure 4, we observed no significant correlation between the circulating levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-10, IL-1β, IFNβ and TGFβ.


Coincident pre-diabetes is associated with dysregulated cytokine responses in pulmonary tuberculosis.

Kumar NP, Banurekha VV, Nair D, Sridhar R, Kornfeld H, Nutman TB, Babu S - PLoS ONE (2014)

No relationship between systemic levels of cytokines and bacterial burdens in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-10, IL-1β, IFNβ, TGFβ and bacterial burdens (as assessed by smear grades - 1+, 2+ and 3+) was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230980&req=5

pone-0112108-g004: No relationship between systemic levels of cytokines and bacterial burdens in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-10, IL-1β, IFNβ, TGFβ and bacterial burdens (as assessed by smear grades - 1+, 2+ and 3+) was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.
Mentions: Since smear grades are an accurate reflection of bacterial burdens, we also sought to examine the relationship between the systemic levels of Type 1, Type 17 and other pro-inflammatory cytokines with the bacterial burdens in the TB-PDM individuals. As shown in Figure 4, we observed no significant correlation between the circulating levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-10, IL-1β, IFNβ and TGFβ.

Bottom Line: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines.However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

View Article: PubMed Central - PubMed

Affiliation: National Institutes of Health-International Center for Excellence in Research, Chennai, India; National Institute for Research in Tuberculosis, Chennai, India.

ABSTRACT

Background: Cytokines play an important role in the pathogenesis of pulmonary tuberculosis (PTB)--Type 2 diabetes mellitus co-morbidity. However, the cytokine interactions that characterize PTB coincident with pre-diabetes (PDM) are not known.

Methods: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).

Results: TB-PDM is characterized by elevated circulating levels of Type 1 (IFNγ, TNFα and IL-2), Type 17 (IL-17A and IL-17F) and other pro-inflammatory (IL-1β, IFNβ and GM-CSF) cytokines. TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines. Moreover, TB antigen stimulated whole blood also showed increased levels of pro-inflammatory (IFNγ, TNFα and IL-1β) cytokines as well. However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

Conclusion: Our data reveal that pre-diabetes in PTB individuals is characterized by heightened cytokine responsiveness, indicating that a balanced pro and anti - inflammatory cytokine milieu is a feature of pre-diabetes--TB co-morbidity.

Show MeSH
Related in: MedlinePlus