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Coincident pre-diabetes is associated with dysregulated cytokine responses in pulmonary tuberculosis.

Kumar NP, Banurekha VV, Nair D, Sridhar R, Kornfeld H, Nutman TB, Babu S - PLoS ONE (2014)

Bottom Line: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines.However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

View Article: PubMed Central - PubMed

Affiliation: National Institutes of Health-International Center for Excellence in Research, Chennai, India; National Institute for Research in Tuberculosis, Chennai, India.

ABSTRACT

Background: Cytokines play an important role in the pathogenesis of pulmonary tuberculosis (PTB)--Type 2 diabetes mellitus co-morbidity. However, the cytokine interactions that characterize PTB coincident with pre-diabetes (PDM) are not known.

Methods: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).

Results: TB-PDM is characterized by elevated circulating levels of Type 1 (IFNγ, TNFα and IL-2), Type 17 (IL-17A and IL-17F) and other pro-inflammatory (IL-1β, IFNβ and GM-CSF) cytokines. TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines. Moreover, TB antigen stimulated whole blood also showed increased levels of pro-inflammatory (IFNγ, TNFα and IL-1β) cytokines as well. However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

Conclusion: Our data reveal that pre-diabetes in PTB individuals is characterized by heightened cytokine responsiveness, indicating that a balanced pro and anti - inflammatory cytokine milieu is a feature of pre-diabetes--TB co-morbidity.

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Related in: MedlinePlus

No relationship between systemic levels of cytokines and HbA1c levels in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-22, GM-CSF, IL-1β and IFNβ and HbA1c levels was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.
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pone-0112108-g003: No relationship between systemic levels of cytokines and HbA1c levels in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-22, GM-CSF, IL-1β and IFNβ and HbA1c levels was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.

Mentions: Since HbA1c is an accurate indicator of the level of glycemic control and increased values reflect poor control, we sought to examine the relationship between the systemic levels of Type 1, Type 17 and other pro-inflammatory cytokines with the degree of glycemic control. To this end, we assessed the association of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-22, IL-1β, GM-CSF and IL-12 with HbA1C levels (in %) in the PDM individuals in the study. As shown in Figure 3, we observed no significant correlation between systemic cytokine levels and the HbA1c levels in TB-PDM individuals.


Coincident pre-diabetes is associated with dysregulated cytokine responses in pulmonary tuberculosis.

Kumar NP, Banurekha VV, Nair D, Sridhar R, Kornfeld H, Nutman TB, Babu S - PLoS ONE (2014)

No relationship between systemic levels of cytokines and HbA1c levels in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-22, GM-CSF, IL-1β and IFNβ and HbA1c levels was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230980&req=5

pone-0112108-g003: No relationship between systemic levels of cytokines and HbA1c levels in TB – PDM individuals.The relationship between the plasma levels of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-22, GM-CSF, IL-1β and IFNβ and HbA1c levels was examined in TB - PDM (n = 48) individuals. The data are represented as scatter plots with each circle representing a single individual. P values were calculated using the Spearman rank correlation.
Mentions: Since HbA1c is an accurate indicator of the level of glycemic control and increased values reflect poor control, we sought to examine the relationship between the systemic levels of Type 1, Type 17 and other pro-inflammatory cytokines with the degree of glycemic control. To this end, we assessed the association of IFNγ, TNFα, IL-2, IL-17A, IL-17F, IL-22, IL-1β, GM-CSF and IL-12 with HbA1C levels (in %) in the PDM individuals in the study. As shown in Figure 3, we observed no significant correlation between systemic cytokine levels and the HbA1c levels in TB-PDM individuals.

Bottom Line: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines.However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

View Article: PubMed Central - PubMed

Affiliation: National Institutes of Health-International Center for Excellence in Research, Chennai, India; National Institute for Research in Tuberculosis, Chennai, India.

ABSTRACT

Background: Cytokines play an important role in the pathogenesis of pulmonary tuberculosis (PTB)--Type 2 diabetes mellitus co-morbidity. However, the cytokine interactions that characterize PTB coincident with pre-diabetes (PDM) are not known.

Methods: To identify the influence of coincident PDM on cytokine levels in PTB, we examined circulating levels of a panel of cytokines in the plasma of individuals with TB-PDM and compared them with those without PDM (TB-NDM).

Results: TB-PDM is characterized by elevated circulating levels of Type 1 (IFNγ, TNFα and IL-2), Type 17 (IL-17A and IL-17F) and other pro-inflammatory (IL-1β, IFNβ and GM-CSF) cytokines. TB-PDM is also characterized by increased systemic levels of Type 2 (IL-5) and regulatory (IL-10 and TGFβ) cytokines. Moreover, TB antigen stimulated whole blood also showed increased levels of pro-inflammatory (IFNγ, TNFα and IL-1β) cytokines as well. However, the cytokines did not exhibit any significant correlation with HbA1C levels or with bacterial burdens.

Conclusion: Our data reveal that pre-diabetes in PTB individuals is characterized by heightened cytokine responsiveness, indicating that a balanced pro and anti - inflammatory cytokine milieu is a feature of pre-diabetes--TB co-morbidity.

Show MeSH
Related in: MedlinePlus