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Serological investigation of food specific immunoglobulin G antibodies in patients with inflammatory bowel diseases.

Cai C, Shen J, Zhao D, Qiao Y, Xu A, Jin S, Ran Z, Zheng Q - PLoS ONE (2014)

Bottom Line: More subjects were found with sensitivity to multiple antigens (≥ 3) in IBD than in HC group (33.9% vs.0.8%, P = 0.000).Patients with multiple positive allergens (≥ 3) were especially found with significant higher total IgG levels compared with sIgG-negative patients (P = 0.003).Age was suggested as a protective factor against the occurrence of sIgG antibodies (P = 0.002).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Gastroenterology & Hepatology, Ministry of Health, Division of Gastroenterology and Hepatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Diseases, 145 Middle Shandong Road, Shanghai 200001, China.

ABSTRACT

Objective: Dietary factors have been indicated to influence the pathogenesis and nature course of inflammatory bowel diseases (IBD) with their wide variances. The aim of the study was to assess the prevalence and clinical significance of 14 serum food specific immunoglobulin G (sIgG) antibodies in patients with IBD.

Methods: This retrospective study comprised a total of 112 patients with IBD, including 79 with Crohn's disease (CD) and 33 with ulcerative colitis (UC). Medical records, clinical data and laboratory results were collected for analysis. Serum IgG antibodies against 14 unique food allergens were detected by semi-quantitative enzyme linked immunosorbent assay (ELISA).

Results: Food sIgG antibodies were detected in 75.9% (60/79) of CD patients, 63.6% (21/33) of UC patients and 33.1% (88/266) of healthy controls (HC). IBD patients showed the significantly higher antibodies prevalence than healthy controls (CD vs. HC, P = 0.000; UC vs. HC, P = 0.001). However no marked difference was observed between CD and UC groups (P = 0.184). More subjects were found with sensitivity to multiple antigens (≥ 3) in IBD than in HC group (33.9% vs.0.8%, P = 0.000). Egg was the most prevalent food allergen. There was a remarkable difference in the levels of general serum IgM (P = 0.045) and IgG (P = 0.041) between patients with positive and negative sIgG antibodies. Patients with multiple positive allergens (≥ 3) were especially found with significant higher total IgG levels compared with sIgG-negative patients (P = 0.003). Age was suggested as a protective factor against the occurrence of sIgG antibodies (P = 0.002).

Conclusions: The study demonstrates a high prevalence of serum IgG antibodies to specific food allergens in patients with IBD. sIgG antibodies may potentially indicate disease status in clinical and be utilized to guide diets for patients.

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Related in: MedlinePlus

Distribution of the number of positive allergen(s) with positive rate of food-specific IgG antibodies in patients with inflammatory bowel diseases (IBD) and healthy controls (HC).Chi-square test, *** P<0.001, n.s. not significant.
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pone-0112154-g002: Distribution of the number of positive allergen(s) with positive rate of food-specific IgG antibodies in patients with inflammatory bowel diseases (IBD) and healthy controls (HC).Chi-square test, *** P<0.001, n.s. not significant.

Mentions: Food specific IgG antibodies were detected positive in 75.9% (60/79) of CD patients, 63.6% (21/33) of UC patients and 33.1% (88/266) of HC (Table 2). The antibodies showed a significantly higher frequency in both CD and UC groups than in healthy controls (CD vs HC, P = 0.000; UC vs HC, P = 0.001) (Figure 1). However, there was no significant difference between CD and UC groups (P = 0.184). In general the total positive rate of all IBD patients was 72.3% (81/112), higher than the control group (P = 0.000). Among them 28.6% (32/112), 9.8% (11/112) and 33.9% (38/112) of subjects were respectively sensitive to one, two and more than two food allergens while the corresponding ratios of healthy controls were 26.7% (71/266), 5.6% (15/266) and 0.8% (2/266). There were more subjects who got intolerant to 3 or more antigens in IBD group than in HC group (P = 0.000) (Figure 2).


Serological investigation of food specific immunoglobulin G antibodies in patients with inflammatory bowel diseases.

Cai C, Shen J, Zhao D, Qiao Y, Xu A, Jin S, Ran Z, Zheng Q - PLoS ONE (2014)

Distribution of the number of positive allergen(s) with positive rate of food-specific IgG antibodies in patients with inflammatory bowel diseases (IBD) and healthy controls (HC).Chi-square test, *** P<0.001, n.s. not significant.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230978&req=5

pone-0112154-g002: Distribution of the number of positive allergen(s) with positive rate of food-specific IgG antibodies in patients with inflammatory bowel diseases (IBD) and healthy controls (HC).Chi-square test, *** P<0.001, n.s. not significant.
Mentions: Food specific IgG antibodies were detected positive in 75.9% (60/79) of CD patients, 63.6% (21/33) of UC patients and 33.1% (88/266) of HC (Table 2). The antibodies showed a significantly higher frequency in both CD and UC groups than in healthy controls (CD vs HC, P = 0.000; UC vs HC, P = 0.001) (Figure 1). However, there was no significant difference between CD and UC groups (P = 0.184). In general the total positive rate of all IBD patients was 72.3% (81/112), higher than the control group (P = 0.000). Among them 28.6% (32/112), 9.8% (11/112) and 33.9% (38/112) of subjects were respectively sensitive to one, two and more than two food allergens while the corresponding ratios of healthy controls were 26.7% (71/266), 5.6% (15/266) and 0.8% (2/266). There were more subjects who got intolerant to 3 or more antigens in IBD group than in HC group (P = 0.000) (Figure 2).

Bottom Line: More subjects were found with sensitivity to multiple antigens (≥ 3) in IBD than in HC group (33.9% vs.0.8%, P = 0.000).Patients with multiple positive allergens (≥ 3) were especially found with significant higher total IgG levels compared with sIgG-negative patients (P = 0.003).Age was suggested as a protective factor against the occurrence of sIgG antibodies (P = 0.002).

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Gastroenterology & Hepatology, Ministry of Health, Division of Gastroenterology and Hepatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Diseases, 145 Middle Shandong Road, Shanghai 200001, China.

ABSTRACT

Objective: Dietary factors have been indicated to influence the pathogenesis and nature course of inflammatory bowel diseases (IBD) with their wide variances. The aim of the study was to assess the prevalence and clinical significance of 14 serum food specific immunoglobulin G (sIgG) antibodies in patients with IBD.

Methods: This retrospective study comprised a total of 112 patients with IBD, including 79 with Crohn's disease (CD) and 33 with ulcerative colitis (UC). Medical records, clinical data and laboratory results were collected for analysis. Serum IgG antibodies against 14 unique food allergens were detected by semi-quantitative enzyme linked immunosorbent assay (ELISA).

Results: Food sIgG antibodies were detected in 75.9% (60/79) of CD patients, 63.6% (21/33) of UC patients and 33.1% (88/266) of healthy controls (HC). IBD patients showed the significantly higher antibodies prevalence than healthy controls (CD vs. HC, P = 0.000; UC vs. HC, P = 0.001). However no marked difference was observed between CD and UC groups (P = 0.184). More subjects were found with sensitivity to multiple antigens (≥ 3) in IBD than in HC group (33.9% vs.0.8%, P = 0.000). Egg was the most prevalent food allergen. There was a remarkable difference in the levels of general serum IgM (P = 0.045) and IgG (P = 0.041) between patients with positive and negative sIgG antibodies. Patients with multiple positive allergens (≥ 3) were especially found with significant higher total IgG levels compared with sIgG-negative patients (P = 0.003). Age was suggested as a protective factor against the occurrence of sIgG antibodies (P = 0.002).

Conclusions: The study demonstrates a high prevalence of serum IgG antibodies to specific food allergens in patients with IBD. sIgG antibodies may potentially indicate disease status in clinical and be utilized to guide diets for patients.

Show MeSH
Related in: MedlinePlus