Roofed grooves: rapid layer engineering of perfusion channels in collagen tissue models.
Bottom Line: In the second part, this was used for effective fabrication of multi-layered plastically compressed collagen constructs with internal channels by roofing the grooves with a second layer.Resulting µ-channels retained their dimensions and were stable over time in culture with fibroblasts and could be cell seeded with a lining layer by simple transfer of epithelial cells.The results of this study provide a valuable platform for rapid fabrication of complex collagen-based tissues in particular for provision of perfusing microchannels through the bulk material for improved core nutrient supply.
Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.Show MeSH
License 1 - License 2 - License 3
Mentions: Once the pattern of collagen fibril deposition and micro-moulded groove formation was understood it was possible to develop a layer fabrication technique (bio-lamination) for producing continuous channels, running through the bulk collagen material. This was achieved by adding a second layer of collagen gel over the first, grooved surface to ‘roof’ the ‘groove’ and convert it into a channel. Figure 5 shows the result of this roofing step. In all the test groove sizes used here, the initial groove remained intact and did not fill with collagen solution, which initially seemed probable. Hence the grooved surfaces were converted by a simple, rapid lamination step into patent channels, suitable for the groove widths used here.Figure 5.
Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.