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Roofed grooves: rapid layer engineering of perfusion channels in collagen tissue models.

Tan NS, Alekseeva T, Brown RA - J Biomater Appl (2014)

Bottom Line: In the second part, this was used for effective fabrication of multi-layered plastically compressed collagen constructs with internal channels by roofing the grooves with a second layer.Resulting µ-channels retained their dimensions and were stable over time in culture with fibroblasts and could be cell seeded with a lining layer by simple transfer of epithelial cells.The results of this study provide a valuable platform for rapid fabrication of complex collagen-based tissues in particular for provision of perfusing microchannels through the bulk material for improved core nutrient supply.

View Article: PubMed Central - PubMed

Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.

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SEM images of patterned constructs, micro-moulded using 25 × 75 μm template (left-hand panel), and 100 × 75 μm template (right-hand panel). Upper row of images shows the overall, low magnification surface pattern of parallel grooves (arrows). Middle and lower rows of images show the transverse structure of individual grooves, with fine (<1 μm) ridges formed perpendicular to the groove axis in the floor and walls (lower high power images).
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fig4-0885328214538865: SEM images of patterned constructs, micro-moulded using 25 × 75 μm template (left-hand panel), and 100 × 75 μm template (right-hand panel). Upper row of images shows the overall, low magnification surface pattern of parallel grooves (arrows). Middle and lower rows of images show the transverse structure of individual grooves, with fine (<1 μm) ridges formed perpendicular to the groove axis in the floor and walls (lower high power images).

Mentions: Figure 4 shows the scanning electron microscope (SEM) structure of two of the rectangular cross-section grooves, 25 and 100 µm (both 75 µm deep). This illustrates the structure and nature of the collagen lamellae which make up the layer and its FLS, together with the stable packing of collagen which was produced. An important observation was the internal micro-scale grooving pattern set into the flat floor of the grooves (inset micrographs). These ridges were perpendicular to the long axis of the groove and continued up the outer vertical walls, reflecting the likely fluid flow around and past the template during compression.Figure 4.


Roofed grooves: rapid layer engineering of perfusion channels in collagen tissue models.

Tan NS, Alekseeva T, Brown RA - J Biomater Appl (2014)

SEM images of patterned constructs, micro-moulded using 25 × 75 μm template (left-hand panel), and 100 × 75 μm template (right-hand panel). Upper row of images shows the overall, low magnification surface pattern of parallel grooves (arrows). Middle and lower rows of images show the transverse structure of individual grooves, with fine (<1 μm) ridges formed perpendicular to the groove axis in the floor and walls (lower high power images).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4230962&req=5

fig4-0885328214538865: SEM images of patterned constructs, micro-moulded using 25 × 75 μm template (left-hand panel), and 100 × 75 μm template (right-hand panel). Upper row of images shows the overall, low magnification surface pattern of parallel grooves (arrows). Middle and lower rows of images show the transverse structure of individual grooves, with fine (<1 μm) ridges formed perpendicular to the groove axis in the floor and walls (lower high power images).
Mentions: Figure 4 shows the scanning electron microscope (SEM) structure of two of the rectangular cross-section grooves, 25 and 100 µm (both 75 µm deep). This illustrates the structure and nature of the collagen lamellae which make up the layer and its FLS, together with the stable packing of collagen which was produced. An important observation was the internal micro-scale grooving pattern set into the flat floor of the grooves (inset micrographs). These ridges were perpendicular to the long axis of the groove and continued up the outer vertical walls, reflecting the likely fluid flow around and past the template during compression.Figure 4.

Bottom Line: In the second part, this was used for effective fabrication of multi-layered plastically compressed collagen constructs with internal channels by roofing the grooves with a second layer.Resulting µ-channels retained their dimensions and were stable over time in culture with fibroblasts and could be cell seeded with a lining layer by simple transfer of epithelial cells.The results of this study provide a valuable platform for rapid fabrication of complex collagen-based tissues in particular for provision of perfusing microchannels through the bulk material for improved core nutrient supply.

View Article: PubMed Central - PubMed

Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.

Show MeSH
Related in: MedlinePlus