Roofed grooves: rapid layer engineering of perfusion channels in collagen tissue models.
Bottom Line: In the second part, this was used for effective fabrication of multi-layered plastically compressed collagen constructs with internal channels by roofing the grooves with a second layer.Resulting µ-channels retained their dimensions and were stable over time in culture with fibroblasts and could be cell seeded with a lining layer by simple transfer of epithelial cells.The results of this study provide a valuable platform for rapid fabrication of complex collagen-based tissues in particular for provision of perfusing microchannels through the bulk material for improved core nutrient supply.
Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.Show MeSH
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Mentions: Figure 4 shows the scanning electron microscope (SEM) structure of two of the rectangular cross-section grooves, 25 and 100 µm (both 75 µm deep). This illustrates the structure and nature of the collagen lamellae which make up the layer and its FLS, together with the stable packing of collagen which was produced. An important observation was the internal micro-scale grooving pattern set into the flat floor of the grooves (inset micrographs). These ridges were perpendicular to the long axis of the groove and continued up the outer vertical walls, reflecting the likely fluid flow around and past the template during compression.Figure 4.
Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.