Roofed grooves: rapid layer engineering of perfusion channels in collagen tissue models.
Bottom Line: In the second part, this was used for effective fabrication of multi-layered plastically compressed collagen constructs with internal channels by roofing the grooves with a second layer.Resulting µ-channels retained their dimensions and were stable over time in culture with fibroblasts and could be cell seeded with a lining layer by simple transfer of epithelial cells.The results of this study provide a valuable platform for rapid fabrication of complex collagen-based tissues in particular for provision of perfusing microchannels through the bulk material for improved core nutrient supply.
Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.Show MeSH
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Mentions: Modified PC protocol was used in this study.10 Briefly, neutralised collagen solution was aliquoted into wells of a 12-well plate (Orange Scientific, Belgium) and allowed to set for 30 min at 37℃. The fluid-removing elements consisted of a spirally wound roll of Whatman chromatography paper (120 cm in length, 4 cm in height (as supplied by the manufacturer, diameter = 22 mm)) and two discs of Whatman filter paper (Whatman UK), cut to the size of the well, which were placed on top of each collagen gel. In order to provide compressive load together with capillary action to drive fluid flow from the collagen gel, each gel was subjected to 29 g initial load, resting on the paper rolls (Figure 1(a)). Once compression was completed, liquid-containing paper rolls were removed, leaving the constructs in their respective wells.Figure 1.
Affiliation: Tissue Repair & Engineering Centre, Institute of Orthopaedics, University College London, United Kingdom.