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Expression of CNPY2 in mouse tissues: quantification and localization.

Hatta K, Guo J, Ludke A, Dhingra S, Singh K, Huang ML, Weisel RD, Li RK - PLoS ONE (2014)

Bottom Line: CNPY2 was also detectable in mouse blood and human and mouse uteri.These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified.Using these new observations we discuss possible functions of the protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Ontario, Canada; Institute of Medical Sciences, University of Toronto, Toronto, Ontario, Canada.

ABSTRACT
Canopy FGF signaling regulator 2 (CNPY2) is a FGF21-modulated protein containing a saposin B-type domain. In vitro studies have shown CNPY2 is able to enhance neurite outgrowth in neurons and stabilize the expression of low density lipoprotein receptor in macrophages and hepatocytes. However, no in vivo data are available on the normal expression of CNPY2 and information is lacking on which cell types express this protein in tissues. To address this, the present study examined CNPY2 expression at the mRNA and protein levels. Quantitative PCR and ELISA examination of mouse tissues showed that CNPY2 varies between organs, with the highest expression in the heart, lung and liver. Immunohistochemistry detected CNPY2 in a variety of cell types including skeletal, cardiac and smooth muscle myocytes, endothelial cells and epithelial cells. CNPY2 was also detectable in mouse blood and human and mouse uteri. These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified. Using these new observations we discuss possible functions of the protein.

No MeSH data available.


CNPY2 is detected in the epithelial cell line BEAS-2B.CNPY2 was detected in BEAS-2B cells using (A) immunofluorescence (CNPY2, green and DAPI, blue) and (B) Western blotting (numbers represent molecular weights, kDa).
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pone-0111370-g007: CNPY2 is detected in the epithelial cell line BEAS-2B.CNPY2 was detected in BEAS-2B cells using (A) immunofluorescence (CNPY2, green and DAPI, blue) and (B) Western blotting (numbers represent molecular weights, kDa).

Mentions: Since we found prominent CNPY2 expression in epithelial cells, we decided to examine whether this protein was expressed in a human epithelial cell line. CNPY2 was detectable by immunofluorescence and Western blotting in the bronchial epithelial cell line BEAS-2B (Figure 7).


Expression of CNPY2 in mouse tissues: quantification and localization.

Hatta K, Guo J, Ludke A, Dhingra S, Singh K, Huang ML, Weisel RD, Li RK - PLoS ONE (2014)

CNPY2 is detected in the epithelial cell line BEAS-2B.CNPY2 was detected in BEAS-2B cells using (A) immunofluorescence (CNPY2, green and DAPI, blue) and (B) Western blotting (numbers represent molecular weights, kDa).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230931&req=5

pone-0111370-g007: CNPY2 is detected in the epithelial cell line BEAS-2B.CNPY2 was detected in BEAS-2B cells using (A) immunofluorescence (CNPY2, green and DAPI, blue) and (B) Western blotting (numbers represent molecular weights, kDa).
Mentions: Since we found prominent CNPY2 expression in epithelial cells, we decided to examine whether this protein was expressed in a human epithelial cell line. CNPY2 was detectable by immunofluorescence and Western blotting in the bronchial epithelial cell line BEAS-2B (Figure 7).

Bottom Line: CNPY2 was also detectable in mouse blood and human and mouse uteri.These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified.Using these new observations we discuss possible functions of the protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Ontario, Canada; Institute of Medical Sciences, University of Toronto, Toronto, Ontario, Canada.

ABSTRACT
Canopy FGF signaling regulator 2 (CNPY2) is a FGF21-modulated protein containing a saposin B-type domain. In vitro studies have shown CNPY2 is able to enhance neurite outgrowth in neurons and stabilize the expression of low density lipoprotein receptor in macrophages and hepatocytes. However, no in vivo data are available on the normal expression of CNPY2 and information is lacking on which cell types express this protein in tissues. To address this, the present study examined CNPY2 expression at the mRNA and protein levels. Quantitative PCR and ELISA examination of mouse tissues showed that CNPY2 varies between organs, with the highest expression in the heart, lung and liver. Immunohistochemistry detected CNPY2 in a variety of cell types including skeletal, cardiac and smooth muscle myocytes, endothelial cells and epithelial cells. CNPY2 was also detectable in mouse blood and human and mouse uteri. These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified. Using these new observations we discuss possible functions of the protein.

No MeSH data available.