Limits...
Expression of CNPY2 in mouse tissues: quantification and localization.

Hatta K, Guo J, Ludke A, Dhingra S, Singh K, Huang ML, Weisel RD, Li RK - PLoS ONE (2014)

Bottom Line: CNPY2 was also detectable in mouse blood and human and mouse uteri.These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified.Using these new observations we discuss possible functions of the protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Ontario, Canada; Institute of Medical Sciences, University of Toronto, Toronto, Ontario, Canada.

ABSTRACT
Canopy FGF signaling regulator 2 (CNPY2) is a FGF21-modulated protein containing a saposin B-type domain. In vitro studies have shown CNPY2 is able to enhance neurite outgrowth in neurons and stabilize the expression of low density lipoprotein receptor in macrophages and hepatocytes. However, no in vivo data are available on the normal expression of CNPY2 and information is lacking on which cell types express this protein in tissues. To address this, the present study examined CNPY2 expression at the mRNA and protein levels. Quantitative PCR and ELISA examination of mouse tissues showed that CNPY2 varies between organs, with the highest expression in the heart, lung and liver. Immunohistochemistry detected CNPY2 in a variety of cell types including skeletal, cardiac and smooth muscle myocytes, endothelial cells and epithelial cells. CNPY2 was also detectable in mouse blood and human and mouse uteri. These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified. Using these new observations we discuss possible functions of the protein.

No MeSH data available.


Related in: MedlinePlus

Luminal localization of CNPY2 in epithelium.High magnification images (objective: 100× oil immersion) show CNPY2 localization in epithelium is luminal in selected tissues.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4230931&req=5

pone-0111370-g005: Luminal localization of CNPY2 in epithelium.High magnification images (objective: 100× oil immersion) show CNPY2 localization in epithelium is luminal in selected tissues.

Mentions: Figure 3 shows CNPY2 staining in the digestive and urinary systems, and in the skin. Figure 4 illustrates CNPY2′s expression in reproductive and brain tissues and various others, including immune tissues such as the bone marrow and spleen as well as the heart, lung and skeletal muscle. CNPY2 was detectable in a variety of tissues (Table 3 and 4), including many types of epithelium throughout the respiratory, digestive and reproductive systems. CNPY2 could often be localized to epithelial cilia but detection was lost in cornified epithelium. CNPY2 was detected in cardiac and skeletal muscle. In the brain, neurons of the hippocampus and cerebral cortex expressed CNPY2. Bone marrow had very weak CNPY2 staining. Occasionally, rare, strongly positive-staining cells were observed in the spleen and lymph nodes. Figure 5 shows higher magnification images that demonstrate that CNPY2 is expressed on the luminal side of various epithelial tissues.


Expression of CNPY2 in mouse tissues: quantification and localization.

Hatta K, Guo J, Ludke A, Dhingra S, Singh K, Huang ML, Weisel RD, Li RK - PLoS ONE (2014)

Luminal localization of CNPY2 in epithelium.High magnification images (objective: 100× oil immersion) show CNPY2 localization in epithelium is luminal in selected tissues.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230931&req=5

pone-0111370-g005: Luminal localization of CNPY2 in epithelium.High magnification images (objective: 100× oil immersion) show CNPY2 localization in epithelium is luminal in selected tissues.
Mentions: Figure 3 shows CNPY2 staining in the digestive and urinary systems, and in the skin. Figure 4 illustrates CNPY2′s expression in reproductive and brain tissues and various others, including immune tissues such as the bone marrow and spleen as well as the heart, lung and skeletal muscle. CNPY2 was detectable in a variety of tissues (Table 3 and 4), including many types of epithelium throughout the respiratory, digestive and reproductive systems. CNPY2 could often be localized to epithelial cilia but detection was lost in cornified epithelium. CNPY2 was detected in cardiac and skeletal muscle. In the brain, neurons of the hippocampus and cerebral cortex expressed CNPY2. Bone marrow had very weak CNPY2 staining. Occasionally, rare, strongly positive-staining cells were observed in the spleen and lymph nodes. Figure 5 shows higher magnification images that demonstrate that CNPY2 is expressed on the luminal side of various epithelial tissues.

Bottom Line: CNPY2 was also detectable in mouse blood and human and mouse uteri.These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified.Using these new observations we discuss possible functions of the protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Ontario, Canada; Institute of Medical Sciences, University of Toronto, Toronto, Ontario, Canada.

ABSTRACT
Canopy FGF signaling regulator 2 (CNPY2) is a FGF21-modulated protein containing a saposin B-type domain. In vitro studies have shown CNPY2 is able to enhance neurite outgrowth in neurons and stabilize the expression of low density lipoprotein receptor in macrophages and hepatocytes. However, no in vivo data are available on the normal expression of CNPY2 and information is lacking on which cell types express this protein in tissues. To address this, the present study examined CNPY2 expression at the mRNA and protein levels. Quantitative PCR and ELISA examination of mouse tissues showed that CNPY2 varies between organs, with the highest expression in the heart, lung and liver. Immunohistochemistry detected CNPY2 in a variety of cell types including skeletal, cardiac and smooth muscle myocytes, endothelial cells and epithelial cells. CNPY2 was also detectable in mouse blood and human and mouse uteri. These data demonstrate CNPY2 is widely distributed in tissues and suggest the protein has biological functions that have yet to be identified. Using these new observations we discuss possible functions of the protein.

No MeSH data available.


Related in: MedlinePlus