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Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa biofilm formation.

Schillaci D, Cusimano MG, Spinello A, Barone G, Russo D, Vitale M, Parrinello D, Arizza V - AMB Express (2014)

Bottom Line: The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics.The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml.We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi, Palermo, 32-90123, Italy.

ABSTRACT
The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiofilm properties against reference strains of Gram positive and Gram negative. The Paracentrin 1 was active against planktonic form of staphylococcal strains (reference and isolates) and Pseudomonas aeruginosa ATCC 15442 at concentrations ranging from 12.5 to 6.2 mg/ml. The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml. We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

No MeSH data available.


Related in: MedlinePlus

Hemolytic activity of SP1 peptides fromParacentrotus lividushemocytes against rabbit blood cells at different concentrations: 1.5 mg/ml; 3.2 mg/ml; 6.2 mg/ml; 50 mg/ml. Data are the mean value of three separate experiments and expressed as percentage of hemolysis ± SD
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Figure 7: Hemolytic activity of SP1 peptides fromParacentrotus lividushemocytes against rabbit blood cells at different concentrations: 1.5 mg/ml; 3.2 mg/ml; 6.2 mg/ml; 50 mg/ml. Data are the mean value of three separate experiments and expressed as percentage of hemolysis ± SD

Mentions: The haemolytic activity of antimicrobial peptides against mammalian erythrocytes is often used as a preliminary evaluation of their selective toxicity and of the interactions of the cationic peptides with negatively charged membranes (Fischer et al. [2003]; Wu et al. [2010]) The performed hemolytic experiment to evaluate the interaction and the potential toxicity of the peptides SP1 did not show a measurable toxic effect against RBC (~1%) at MIC concentrations. A slight haemolytic activity, about 11.8%, was evident only at the highest concentration (50 mg/ml) (Figure 7).


Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa biofilm formation.

Schillaci D, Cusimano MG, Spinello A, Barone G, Russo D, Vitale M, Parrinello D, Arizza V - AMB Express (2014)

Hemolytic activity of SP1 peptides fromParacentrotus lividushemocytes against rabbit blood cells at different concentrations: 1.5 mg/ml; 3.2 mg/ml; 6.2 mg/ml; 50 mg/ml. Data are the mean value of three separate experiments and expressed as percentage of hemolysis ± SD
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230904&req=5

Figure 7: Hemolytic activity of SP1 peptides fromParacentrotus lividushemocytes against rabbit blood cells at different concentrations: 1.5 mg/ml; 3.2 mg/ml; 6.2 mg/ml; 50 mg/ml. Data are the mean value of three separate experiments and expressed as percentage of hemolysis ± SD
Mentions: The haemolytic activity of antimicrobial peptides against mammalian erythrocytes is often used as a preliminary evaluation of their selective toxicity and of the interactions of the cationic peptides with negatively charged membranes (Fischer et al. [2003]; Wu et al. [2010]) The performed hemolytic experiment to evaluate the interaction and the potential toxicity of the peptides SP1 did not show a measurable toxic effect against RBC (~1%) at MIC concentrations. A slight haemolytic activity, about 11.8%, was evident only at the highest concentration (50 mg/ml) (Figure 7).

Bottom Line: The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics.The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml.We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi, Palermo, 32-90123, Italy.

ABSTRACT
The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiofilm properties against reference strains of Gram positive and Gram negative. The Paracentrin 1 was active against planktonic form of staphylococcal strains (reference and isolates) and Pseudomonas aeruginosa ATCC 15442 at concentrations ranging from 12.5 to 6.2 mg/ml. The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml. We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

No MeSH data available.


Related in: MedlinePlus