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Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa biofilm formation.

Schillaci D, Cusimano MG, Spinello A, Barone G, Russo D, Vitale M, Parrinello D, Arizza V - AMB Express (2014)

Bottom Line: The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics.The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml.We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi, Palermo, 32-90123, Italy.

ABSTRACT
The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiofilm properties against reference strains of Gram positive and Gram negative. The Paracentrin 1 was active against planktonic form of staphylococcal strains (reference and isolates) and Pseudomonas aeruginosa ATCC 15442 at concentrations ranging from 12.5 to 6.2 mg/ml. The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml. We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

No MeSH data available.


Related in: MedlinePlus

SEM micrography showing the effect of sub-MIC concentration of SP1 onS. epidermidisRP62A biofilm formation. A) Growth control (not treated with SP1); B) sample treated with a sub-MIC concentration (3.1 mg/ml) of SP1.
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Figure 6: SEM micrography showing the effect of sub-MIC concentration of SP1 onS. epidermidisRP62A biofilm formation. A) Growth control (not treated with SP1); B) sample treated with a sub-MIC concentration (3.1 mg/ml) of SP1.

Mentions: SEM analysis was carried out to visualize the detailed architecture of biofilm and the antibiofilm effects of SP1 on bacterial biofilm of S. epidermidis RP62A (Figure 6). The control biofilm after 24 h was composed by multilayered conglomerated bacterial cells clusters that produced a dense biofilm matrix (Figure 6A). Sub MIC concentration (3.1 mg/ml) of SP1 was able to inhibit the formation of a multistratified structure, in fact in the sample treated with SP1 we found few monostratified bacterial cell clusters respect to the growth control (Figure 6B).


Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa biofilm formation.

Schillaci D, Cusimano MG, Spinello A, Barone G, Russo D, Vitale M, Parrinello D, Arizza V - AMB Express (2014)

SEM micrography showing the effect of sub-MIC concentration of SP1 onS. epidermidisRP62A biofilm formation. A) Growth control (not treated with SP1); B) sample treated with a sub-MIC concentration (3.1 mg/ml) of SP1.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230904&req=5

Figure 6: SEM micrography showing the effect of sub-MIC concentration of SP1 onS. epidermidisRP62A biofilm formation. A) Growth control (not treated with SP1); B) sample treated with a sub-MIC concentration (3.1 mg/ml) of SP1.
Mentions: SEM analysis was carried out to visualize the detailed architecture of biofilm and the antibiofilm effects of SP1 on bacterial biofilm of S. epidermidis RP62A (Figure 6). The control biofilm after 24 h was composed by multilayered conglomerated bacterial cells clusters that produced a dense biofilm matrix (Figure 6A). Sub MIC concentration (3.1 mg/ml) of SP1 was able to inhibit the formation of a multistratified structure, in fact in the sample treated with SP1 we found few monostratified bacterial cell clusters respect to the growth control (Figure 6B).

Bottom Line: The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics.The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml.We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi, Palermo, 32-90123, Italy.

ABSTRACT
The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiofilm properties against reference strains of Gram positive and Gram negative. The Paracentrin 1 was active against planktonic form of staphylococcal strains (reference and isolates) and Pseudomonas aeruginosa ATCC 15442 at concentrations ranging from 12.5 to 6.2 mg/ml. The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml. We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

No MeSH data available.


Related in: MedlinePlus