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Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa biofilm formation.

Schillaci D, Cusimano MG, Spinello A, Barone G, Russo D, Vitale M, Parrinello D, Arizza V - AMB Express (2014)

Bottom Line: The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics.The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml.We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi, Palermo, 32-90123, Italy.

ABSTRACT
The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiofilm properties against reference strains of Gram positive and Gram negative. The Paracentrin 1 was active against planktonic form of staphylococcal strains (reference and isolates) and Pseudomonas aeruginosa ATCC 15442 at concentrations ranging from 12.5 to 6.2 mg/ml. The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml. We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

No MeSH data available.


Related in: MedlinePlus

Interference with biofilm formation of SP1 against reference staphylococcal andP. aeruginosastrains. The percentage of inhibition were evaluated comparing the samples with not-treated 24 h old biofilms and staining with safranin. Bacterial strains () S. aureus 25923, () S. aureus 29213, () S. aureus 6538, () S. epidermidis RP62A, () P. aeruginosa 15442. Data for each strain are the mean of three distinct experiments ± S.D.
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Figure 5: Interference with biofilm formation of SP1 against reference staphylococcal andP. aeruginosastrains. The percentage of inhibition were evaluated comparing the samples with not-treated 24 h old biofilms and staining with safranin. Bacterial strains () S. aureus 25923, () S. aureus 29213, () S. aureus 6538, () S. epidermidis RP62A, () P. aeruginosa 15442. Data for each strain are the mean of three distinct experiments ± S.D.

Mentions: The interference with biofilm formation of SP1 against staphylococcal reference strains as S. aureus 25923, S. aureus 29213, S. aureus 6538, S. epidermidis RP62A and P. aeruginosa 15442 was observed. The inhibition was very evident to the highest concentrations of SP1 at 6.2 mg/ml, when the values, for all strains, reached approximately 80%. At the lowest concentrations of SP1 the degree of inhibition is reduced by following a dose dependence. P. aeruginosa strain 15442 was the most sensitive inhibitory activity of SP1, in fact, at a concentration of 3.1 mg/ml, a value of about 73% inhibition was observed, while for the other strains the inhibition values were of around 50% (Figure 5).


Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa biofilm formation.

Schillaci D, Cusimano MG, Spinello A, Barone G, Russo D, Vitale M, Parrinello D, Arizza V - AMB Express (2014)

Interference with biofilm formation of SP1 against reference staphylococcal andP. aeruginosastrains. The percentage of inhibition were evaluated comparing the samples with not-treated 24 h old biofilms and staining with safranin. Bacterial strains () S. aureus 25923, () S. aureus 29213, () S. aureus 6538, () S. epidermidis RP62A, () P. aeruginosa 15442. Data for each strain are the mean of three distinct experiments ± S.D.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230904&req=5

Figure 5: Interference with biofilm formation of SP1 against reference staphylococcal andP. aeruginosastrains. The percentage of inhibition were evaluated comparing the samples with not-treated 24 h old biofilms and staining with safranin. Bacterial strains () S. aureus 25923, () S. aureus 29213, () S. aureus 6538, () S. epidermidis RP62A, () P. aeruginosa 15442. Data for each strain are the mean of three distinct experiments ± S.D.
Mentions: The interference with biofilm formation of SP1 against staphylococcal reference strains as S. aureus 25923, S. aureus 29213, S. aureus 6538, S. epidermidis RP62A and P. aeruginosa 15442 was observed. The inhibition was very evident to the highest concentrations of SP1 at 6.2 mg/ml, when the values, for all strains, reached approximately 80%. At the lowest concentrations of SP1 the degree of inhibition is reduced by following a dose dependence. P. aeruginosa strain 15442 was the most sensitive inhibitory activity of SP1, in fact, at a concentration of 3.1 mg/ml, a value of about 73% inhibition was observed, while for the other strains the inhibition values were of around 50% (Figure 5).

Bottom Line: The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics.The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml.We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dip. STEBICEF, Università degli Studi di Palermo, Via Archirafi, Palermo, 32-90123, Italy.

ABSTRACT
The rise of antibiotic-resistance as well as the reduction of investments by pharmaceutical companies in the development of new antibiotics have stimulated the investigation for alternative strategies to conventional antibiotics. Many antimicrobial peptides show a high specificity for prokaryotes and a low toxicity for eukaryotic cells and, due to their mode of action the development of resistance is considered unlikely. We recently characterized an antimicrobial peptide that was called Paracentrin 1 from the 5-kDa peptide fraction from the coelomocyte cytosol of the Paracentrotus lividus. In this study, the chemically synthesized Paracentrin 1, was tested for its antimicrobial and antibiofilm properties against reference strains of Gram positive and Gram negative. The Paracentrin 1 was active against planktonic form of staphylococcal strains (reference and isolates) and Pseudomonas aeruginosa ATCC 15442 at concentrations ranging from 12.5 to 6.2 mg/ml. The Paracentrin 1 was able to inhibit biofilm formation of staphylococcal and Pseudomonas aeruginosa strains at concentrations ranging from 3.1 to 0.75 mg/ml. We consider the tested peptide as a good starting molecule for novel synthetic derivatives with improved pharmaceutical potential.

No MeSH data available.


Related in: MedlinePlus