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Diversity and biochemical features of culturable fungi from the coastal waters of Southern China.

Li L, Singh P, Liu Y, Pan S, Wang G - AMB Express (2014)

Bottom Line: A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples.Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase.Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China ; Department of Biological Science, National University of Singapore, Singapore 117543, Singapore.

ABSTRACT
Fungi play a major role in various biogeochemical cycles of terrestrial and marine ecosystems. However, fungi in marine environments remain to be one of the most under-studied microbial groups. This study investigates the diversity of planktonic fungi from the coastal habitat off Pearl River Delta (China) using culture-dependent approach. A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples. Microscopic and ITS rRNA gene sequence analyses revealed that most of the fungi belonged to the phylum Ascomycota and Basidiomycota with a very small percentage (3%) of the subphylum Mucoromycotina of the Phylum Zygomycota. Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase. Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

No MeSH data available.


Qualitative assay of enzyme activities of fungi on substrate specific media plates. A. Plate assay for cellulose activities (PKU Y7 Rhodotorula mucilaginosa sp. (left) and PKU Y9 Rhodotorula mucilaginosa sp. showing good cellulose activity (rght)); B. Plate assay for laccase activities (PKU F11 Arthrinium phaeospermum sp. showing no laccase activity (left); PKU F16 Cladosporium sphaerospermum showing considerable laccase activities (right)); C. Plate assay for lipase activities on plate (PKU Y7 Rhodotorula mucilaginosa sp. showing little lipase activity (left); PKU Y9 Rhodotorula mucilaginosa sp. showing good lipase activity (right)).
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Figure 3: Qualitative assay of enzyme activities of fungi on substrate specific media plates. A. Plate assay for cellulose activities (PKU Y7 Rhodotorula mucilaginosa sp. (left) and PKU Y9 Rhodotorula mucilaginosa sp. showing good cellulose activity (rght)); B. Plate assay for laccase activities (PKU F11 Arthrinium phaeospermum sp. showing no laccase activity (left); PKU F16 Cladosporium sphaerospermum showing considerable laccase activities (right)); C. Plate assay for lipase activities on plate (PKU Y7 Rhodotorula mucilaginosa sp. showing little lipase activity (left); PKU Y9 Rhodotorula mucilaginosa sp. showing good lipase activity (right)).

Mentions: Qualitative analyses of different enzymes demonstrated positive result for most of the fungal isolates (Table 3, Figure 3). The majority of fungal isolates (~84%) exhibited cellulase and lipase activities. In comparison, only a few of the isolates (~38%) showed positive laccase activity (Table 3, Figure 3). The quantitative analyses for production of these three enzymes were done for four of these fungal isolates (Table 4). Cladosporium sp. (PKU F16), Rhodosporidium sp. (PKU Y5) and Candida sp. (PKU Y8) showed considerable production of lipase enzyme, reaching up to 21.94 U ml−1 for Candida sp. In contrast, cellulase production was not very high for any of these isolates, being maximum of 1.3 U ml−1 demonstrated by Candida sp. PKU F16 (Cladosporium sp.) and PKU F18 (Ascomycota sp.) were the best producers of laccase enzyme showing activities of 14.6 U ml−1 and 10.5 U ml−1 respectively (Table 4). All the isolates showed maximum production of these extracellular enzymes on 6th day of their growth (Table 4).


Diversity and biochemical features of culturable fungi from the coastal waters of Southern China.

Li L, Singh P, Liu Y, Pan S, Wang G - AMB Express (2014)

Qualitative assay of enzyme activities of fungi on substrate specific media plates. A. Plate assay for cellulose activities (PKU Y7 Rhodotorula mucilaginosa sp. (left) and PKU Y9 Rhodotorula mucilaginosa sp. showing good cellulose activity (rght)); B. Plate assay for laccase activities (PKU F11 Arthrinium phaeospermum sp. showing no laccase activity (left); PKU F16 Cladosporium sphaerospermum showing considerable laccase activities (right)); C. Plate assay for lipase activities on plate (PKU Y7 Rhodotorula mucilaginosa sp. showing little lipase activity (left); PKU Y9 Rhodotorula mucilaginosa sp. showing good lipase activity (right)).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230900&req=5

Figure 3: Qualitative assay of enzyme activities of fungi on substrate specific media plates. A. Plate assay for cellulose activities (PKU Y7 Rhodotorula mucilaginosa sp. (left) and PKU Y9 Rhodotorula mucilaginosa sp. showing good cellulose activity (rght)); B. Plate assay for laccase activities (PKU F11 Arthrinium phaeospermum sp. showing no laccase activity (left); PKU F16 Cladosporium sphaerospermum showing considerable laccase activities (right)); C. Plate assay for lipase activities on plate (PKU Y7 Rhodotorula mucilaginosa sp. showing little lipase activity (left); PKU Y9 Rhodotorula mucilaginosa sp. showing good lipase activity (right)).
Mentions: Qualitative analyses of different enzymes demonstrated positive result for most of the fungal isolates (Table 3, Figure 3). The majority of fungal isolates (~84%) exhibited cellulase and lipase activities. In comparison, only a few of the isolates (~38%) showed positive laccase activity (Table 3, Figure 3). The quantitative analyses for production of these three enzymes were done for four of these fungal isolates (Table 4). Cladosporium sp. (PKU F16), Rhodosporidium sp. (PKU Y5) and Candida sp. (PKU Y8) showed considerable production of lipase enzyme, reaching up to 21.94 U ml−1 for Candida sp. In contrast, cellulase production was not very high for any of these isolates, being maximum of 1.3 U ml−1 demonstrated by Candida sp. PKU F16 (Cladosporium sp.) and PKU F18 (Ascomycota sp.) were the best producers of laccase enzyme showing activities of 14.6 U ml−1 and 10.5 U ml−1 respectively (Table 4). All the isolates showed maximum production of these extracellular enzymes on 6th day of their growth (Table 4).

Bottom Line: A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples.Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase.Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China ; Department of Biological Science, National University of Singapore, Singapore 117543, Singapore.

ABSTRACT
Fungi play a major role in various biogeochemical cycles of terrestrial and marine ecosystems. However, fungi in marine environments remain to be one of the most under-studied microbial groups. This study investigates the diversity of planktonic fungi from the coastal habitat off Pearl River Delta (China) using culture-dependent approach. A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples. Microscopic and ITS rRNA gene sequence analyses revealed that most of the fungi belonged to the phylum Ascomycota and Basidiomycota with a very small percentage (3%) of the subphylum Mucoromycotina of the Phylum Zygomycota. Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase. Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

No MeSH data available.