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Diversity and biochemical features of culturable fungi from the coastal waters of Southern China.

Li L, Singh P, Liu Y, Pan S, Wang G - AMB Express (2014)

Bottom Line: A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples.Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase.Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China ; Department of Biological Science, National University of Singapore, Singapore 117543, Singapore.

ABSTRACT
Fungi play a major role in various biogeochemical cycles of terrestrial and marine ecosystems. However, fungi in marine environments remain to be one of the most under-studied microbial groups. This study investigates the diversity of planktonic fungi from the coastal habitat off Pearl River Delta (China) using culture-dependent approach. A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples. Microscopic and ITS rRNA gene sequence analyses revealed that most of the fungi belonged to the phylum Ascomycota and Basidiomycota with a very small percentage (3%) of the subphylum Mucoromycotina of the Phylum Zygomycota. Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase. Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

No MeSH data available.


NJ phylogenetic tree based on ITS rRNA genes from 22 fungi and 9 yeast derived from coastal ecosystems of Pearl River Delta. Numbers at nodes indicate bootstrap values of neighborjoining analysis for 1,000 replicates (values below 50% not shown).
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Figure 2: NJ phylogenetic tree based on ITS rRNA genes from 22 fungi and 9 yeast derived from coastal ecosystems of Pearl River Delta. Numbers at nodes indicate bootstrap values of neighborjoining analysis for 1,000 replicates (values below 50% not shown).

Mentions: Fungi were isolated from all the seawater depths in the present study (Table 1). However, maximum numbers of fungal colonies were recovered from surface sediments (0 m depth) and seawater samples at 10 m depth. Fungi were also recovered from sediment samples using particle plating technique (Table 1). SDA was found to be better media than MEA for the isolation of fungi (Table 1). However, there was no statistical significance observed between different depths, media and the number of fungal colonies. A total of 22 fungi and 9 yeasts were isolated in this study. The fungal isolates mostly belonged to Ascomycota, Basidiomycota and Zygomycota based on ITS rRNA gene analysis (Table 2, Figure 1). Fungal isolates showing similarity with the phylum Ascomycota were dominating among above three, accounting for 74%. Members of Basidiomycota and Zygomycota made up for 23% and 3%, respectively (Figure 1). Of the Ascomycetes, isolates belonged to 13 genera i.e. Aspergillus, Hypocraea, Arthrinium, Diaporthe, Phoma, Trichoderma, Dothideomycetes, Cladosporium, Curvularia, Pleosporales, Pyrenochaeta, Aureobasidium and Candida. Isolates of Basidiomycota were affiliated with Rhodotorula, Rhodosporidium and Trichosporon sp. Only Mucor sp. of Zygomycota was identifed in this study (Table 2, Figure 2). All the fungal ITS rRNA gene sequences showed 100 or 99% identity with the existing sequences of NCBI database except PKU F18, showing 92% identity with Ascomycota sp. AR-2010 (Table 2).


Diversity and biochemical features of culturable fungi from the coastal waters of Southern China.

Li L, Singh P, Liu Y, Pan S, Wang G - AMB Express (2014)

NJ phylogenetic tree based on ITS rRNA genes from 22 fungi and 9 yeast derived from coastal ecosystems of Pearl River Delta. Numbers at nodes indicate bootstrap values of neighborjoining analysis for 1,000 replicates (values below 50% not shown).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230900&req=5

Figure 2: NJ phylogenetic tree based on ITS rRNA genes from 22 fungi and 9 yeast derived from coastal ecosystems of Pearl River Delta. Numbers at nodes indicate bootstrap values of neighborjoining analysis for 1,000 replicates (values below 50% not shown).
Mentions: Fungi were isolated from all the seawater depths in the present study (Table 1). However, maximum numbers of fungal colonies were recovered from surface sediments (0 m depth) and seawater samples at 10 m depth. Fungi were also recovered from sediment samples using particle plating technique (Table 1). SDA was found to be better media than MEA for the isolation of fungi (Table 1). However, there was no statistical significance observed between different depths, media and the number of fungal colonies. A total of 22 fungi and 9 yeasts were isolated in this study. The fungal isolates mostly belonged to Ascomycota, Basidiomycota and Zygomycota based on ITS rRNA gene analysis (Table 2, Figure 1). Fungal isolates showing similarity with the phylum Ascomycota were dominating among above three, accounting for 74%. Members of Basidiomycota and Zygomycota made up for 23% and 3%, respectively (Figure 1). Of the Ascomycetes, isolates belonged to 13 genera i.e. Aspergillus, Hypocraea, Arthrinium, Diaporthe, Phoma, Trichoderma, Dothideomycetes, Cladosporium, Curvularia, Pleosporales, Pyrenochaeta, Aureobasidium and Candida. Isolates of Basidiomycota were affiliated with Rhodotorula, Rhodosporidium and Trichosporon sp. Only Mucor sp. of Zygomycota was identifed in this study (Table 2, Figure 2). All the fungal ITS rRNA gene sequences showed 100 or 99% identity with the existing sequences of NCBI database except PKU F18, showing 92% identity with Ascomycota sp. AR-2010 (Table 2).

Bottom Line: A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples.Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase.Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China ; Department of Biological Science, National University of Singapore, Singapore 117543, Singapore.

ABSTRACT
Fungi play a major role in various biogeochemical cycles of terrestrial and marine ecosystems. However, fungi in marine environments remain to be one of the most under-studied microbial groups. This study investigates the diversity of planktonic fungi from the coastal habitat off Pearl River Delta (China) using culture-dependent approach. A total of 22 fungi and 9 yeast isolates were recovered from 30 seawater and 2 sediment samples. Microscopic and ITS rRNA gene sequence analyses revealed that most of the fungi belonged to the phylum Ascomycota and Basidiomycota with a very small percentage (3%) of the subphylum Mucoromycotina of the Phylum Zygomycota. Most of these fungal isolates exhibited considerable production of extracellular enzymes, cellulase, lipase and laccase. Fungal isolates of two genera Mucor and Aspergillus sp. demonstrated pelletization capability over a wide range of pH, suggesting them as potential agents towards algae harvesting and wastewater treatment.

No MeSH data available.