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Effect of high pressure on hydrocarbon-degrading bacteria.

Schedler M, Hiessl R, Valladares Juárez AG, Gust G, Müller R - AMB Express (2014)

Bottom Line: However, above this pressure growth decreased and at 12 MPa or more no more growth was observed.Nevertheless, S. yanoikuyae continued to convert naphthalene at pressure >12 MPa, although at a lower rate than at 0.1 MPa.These results show that high pressure has a strong influence on the biodegradation of crude oil components and that, contrary to previous assumptions, the role of pressure cannot be discounted when estimating the biodegradation and ultimate fate of deep-sea oil releases such as the Deepwater Horizon event.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg 21073, Germany.

ABSTRACT
The blowout of the Deepwater Horizon in the Gulf of Mexico in 2010 occurred at a depth of 1500 m, corresponding to a hydrostatic pressure of 15 MPa. Up to now, knowledge about the impact of high pressure on oil-degrading bacteria has been scarce. To investigate how the biodegradation of crude oil and its components is influenced by high pressures, like those in deep-sea environments, hydrocarbon degradation and growth of two model strains were studied in high-pressure reactors. The alkane-degrading strain Rhodococcus qingshengii TUHH-12 grew well on n-hexadecane at 15 MPa at a rate of 0.16 h(-1), although slightly slower than at ambient pressure (0.36 h(-1)). In contrast, the growth of the aromatic hydrocarbon degrading strain Sphingobium yanoikuyae B1 was highly affected by elevated pressures. Pressures of up to 8.8 MPa had little effect on growth of this strain. However, above this pressure growth decreased and at 12 MPa or more no more growth was observed. Nevertheless, S. yanoikuyae continued to convert naphthalene at pressure >12 MPa, although at a lower rate than at 0.1 MPa. This suggests that certain metabolic functions of this bacterium were inhibited by pressure to a greater extent than the enzymes responsible for naphthalene degradation. These results show that high pressure has a strong influence on the biodegradation of crude oil components and that, contrary to previous assumptions, the role of pressure cannot be discounted when estimating the biodegradation and ultimate fate of deep-sea oil releases such as the Deepwater Horizon event.

No MeSH data available.


Related in: MedlinePlus

Growth ofS. yanoikuyaeB1 on naphthalene at 0.1 MPa vs. 13.9 MPa.S. yanoikuyae B1 was cultivated at room temperature. CFUs were determined in triplicate and standard deviations are shown. △ CFU at 0.1 MPa, ▲ CFU at 13.9 MPa, □ concentration of naphthalene at 0.1 MPa, ■ concentration of naphthalene at 13.9 MPa.
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Figure 3: Growth ofS. yanoikuyaeB1 on naphthalene at 0.1 MPa vs. 13.9 MPa.S. yanoikuyae B1 was cultivated at room temperature. CFUs were determined in triplicate and standard deviations are shown. △ CFU at 0.1 MPa, ▲ CFU at 13.9 MPa, □ concentration of naphthalene at 0.1 MPa, ■ concentration of naphthalene at 13.9 MPa.

Mentions: S. yanoikuyae B1 was incubated on naphthalene at high pressure (13.9 MPa) and at atmospheric pressure. The growth of S. yanoikuyae B1 on this PAH was strongly inhibited by high pressure. Bacteria grew at 0.1 MPa with a lag phase of 15 h, an exponential phase with a growth rate of 0.33 h−1 (from 15 to 28 h) and reached stationary phase at 28 h of incubation (Figure 3). At 13.9 MPa, however, CFUs of S. yanoikuyae B1 decreased after 15 h cultivation time until no CFUs could be counted after 66 h.


Effect of high pressure on hydrocarbon-degrading bacteria.

Schedler M, Hiessl R, Valladares Juárez AG, Gust G, Müller R - AMB Express (2014)

Growth ofS. yanoikuyaeB1 on naphthalene at 0.1 MPa vs. 13.9 MPa.S. yanoikuyae B1 was cultivated at room temperature. CFUs were determined in triplicate and standard deviations are shown. △ CFU at 0.1 MPa, ▲ CFU at 13.9 MPa, □ concentration of naphthalene at 0.1 MPa, ■ concentration of naphthalene at 13.9 MPa.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230898&req=5

Figure 3: Growth ofS. yanoikuyaeB1 on naphthalene at 0.1 MPa vs. 13.9 MPa.S. yanoikuyae B1 was cultivated at room temperature. CFUs were determined in triplicate and standard deviations are shown. △ CFU at 0.1 MPa, ▲ CFU at 13.9 MPa, □ concentration of naphthalene at 0.1 MPa, ■ concentration of naphthalene at 13.9 MPa.
Mentions: S. yanoikuyae B1 was incubated on naphthalene at high pressure (13.9 MPa) and at atmospheric pressure. The growth of S. yanoikuyae B1 on this PAH was strongly inhibited by high pressure. Bacteria grew at 0.1 MPa with a lag phase of 15 h, an exponential phase with a growth rate of 0.33 h−1 (from 15 to 28 h) and reached stationary phase at 28 h of incubation (Figure 3). At 13.9 MPa, however, CFUs of S. yanoikuyae B1 decreased after 15 h cultivation time until no CFUs could be counted after 66 h.

Bottom Line: However, above this pressure growth decreased and at 12 MPa or more no more growth was observed.Nevertheless, S. yanoikuyae continued to convert naphthalene at pressure >12 MPa, although at a lower rate than at 0.1 MPa.These results show that high pressure has a strong influence on the biodegradation of crude oil components and that, contrary to previous assumptions, the role of pressure cannot be discounted when estimating the biodegradation and ultimate fate of deep-sea oil releases such as the Deepwater Horizon event.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg 21073, Germany.

ABSTRACT
The blowout of the Deepwater Horizon in the Gulf of Mexico in 2010 occurred at a depth of 1500 m, corresponding to a hydrostatic pressure of 15 MPa. Up to now, knowledge about the impact of high pressure on oil-degrading bacteria has been scarce. To investigate how the biodegradation of crude oil and its components is influenced by high pressures, like those in deep-sea environments, hydrocarbon degradation and growth of two model strains were studied in high-pressure reactors. The alkane-degrading strain Rhodococcus qingshengii TUHH-12 grew well on n-hexadecane at 15 MPa at a rate of 0.16 h(-1), although slightly slower than at ambient pressure (0.36 h(-1)). In contrast, the growth of the aromatic hydrocarbon degrading strain Sphingobium yanoikuyae B1 was highly affected by elevated pressures. Pressures of up to 8.8 MPa had little effect on growth of this strain. However, above this pressure growth decreased and at 12 MPa or more no more growth was observed. Nevertheless, S. yanoikuyae continued to convert naphthalene at pressure >12 MPa, although at a lower rate than at 0.1 MPa. This suggests that certain metabolic functions of this bacterium were inhibited by pressure to a greater extent than the enzymes responsible for naphthalene degradation. These results show that high pressure has a strong influence on the biodegradation of crude oil components and that, contrary to previous assumptions, the role of pressure cannot be discounted when estimating the biodegradation and ultimate fate of deep-sea oil releases such as the Deepwater Horizon event.

No MeSH data available.


Related in: MedlinePlus