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Co-production of bioethanol and probiotic yeast biomass from agricultural feedstock: application of the rural biorefinery concept.

Hull CM, Loveridge EJ, Donnison IS, Kelly DE, Kelly SL - AMB Express (2014)

Bottom Line: Maximum product yields for MYA-769 (39.18 [±2.42] mg ethanol mL(-1) and 4.96 [±0.15] g dry weight L(-1)) compared closely to those of Turbo (37.43 [±1.99] mg mL(-1) and 4.78 [±0.10] g L(-1), respectively).Co-production, marketing and/or on-site utilisation of probiotic yeast biomass as a direct-fed microbial to improve livestock health represents a novel and viable prospect for rural biorefineries.Given emergent evidence to suggest that dietary yeast supplementations might also mitigate ruminant enteric methane emissions, the administration of probiotic yeast biomass could also offer an economically feasible way of reducing atmospheric CH4.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Life Science, College of Medicine, Swansea University, Swansea SA2 8PP, Wales, UK.

ABSTRACT
Microbial biotechnology and biotransformations promise to diversify the scope of the biorefinery approach for the production of high-value products and biofuels from industrial, rural and municipal waste feedstocks. In addition to bio-based chemicals and metabolites, microbial biomass itself constitutes an obvious but overlooked by-product of existing biofermentation systems which warrants fuller attention. The probiotic yeast Saccharomyces boulardii is used to treat gastrointestinal disorders and marketed as a human health supplement. Despite its relatedness to S. cerevisiae that is employed widely in biotechnology, food and biofuel industries, the alternative applications of S. boulardii are not well studied. Using a biorefinery approach, we compared the bioethanol and biomass yields attainable from agriculturally-sourced grass juice using probiotic S. boulardii (strain MYA-769) and a commercial S. cerevisiae brewing strain (Turbo yeast). Maximum product yields for MYA-769 (39.18 [±2.42] mg ethanol mL(-1) and 4.96 [±0.15] g dry weight L(-1)) compared closely to those of Turbo (37.43 [±1.99] mg mL(-1) and 4.78 [±0.10] g L(-1), respectively). Co-production, marketing and/or on-site utilisation of probiotic yeast biomass as a direct-fed microbial to improve livestock health represents a novel and viable prospect for rural biorefineries. Given emergent evidence to suggest that dietary yeast supplementations might also mitigate ruminant enteric methane emissions, the administration of probiotic yeast biomass could also offer an economically feasible way of reducing atmospheric CH4.

No MeSH data available.


Related in: MedlinePlus

Mean [±S.D] glucose, fructose and fructan concentrations in untreated GJ (open bars) and GJ + tfosEp (filled bars).a Fructan = polyfructose.
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Figure 1: Mean [±S.D] glucose, fructose and fructan concentrations in untreated GJ (open bars) and GJ + tfosEp (filled bars).a Fructan = polyfructose.

Mentions: Pre-treatment of grass juice (GJ) feedstock with the soluble, truncated core domain of Lactobacillus paracasei β-fructosidase (tfosEp) purified from recombinant Escherichia coli (Martel et al. [2010]) resulted in the complete hydrolysis of non-fermentable fructan moieties (Figure 1). The total monosaccharide (glucose and fructose) content of GJ + tfosEp (73.31 [±0.67] mg mL−1) was over two-fold higher than that of untreated GJ (30.39 [±1.51] mg mL−1); the sucrose content of both was negligible (0.60 [±0.06] mg mL−1). Grass juice contains smaller amounts of other sugars (e.g., galactooligosaccharides and maltosaccharides) in addition to proteins which can also be used for fermentation and growth; non-fermentable carbohydrates (i.e., lignin, cellulose and hemicellulose) are found in grass pulp and the fibrous biomass fraction (Charlton et al. [2009]). GJ + tfosEp was found to support optimal yeast growth, bioethanol and biomass production and was consequently employed as the feedstock for all experimental work reported in the present study.


Co-production of bioethanol and probiotic yeast biomass from agricultural feedstock: application of the rural biorefinery concept.

Hull CM, Loveridge EJ, Donnison IS, Kelly DE, Kelly SL - AMB Express (2014)

Mean [±S.D] glucose, fructose and fructan concentrations in untreated GJ (open bars) and GJ + tfosEp (filled bars).a Fructan = polyfructose.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230830&req=5

Figure 1: Mean [±S.D] glucose, fructose and fructan concentrations in untreated GJ (open bars) and GJ + tfosEp (filled bars).a Fructan = polyfructose.
Mentions: Pre-treatment of grass juice (GJ) feedstock with the soluble, truncated core domain of Lactobacillus paracasei β-fructosidase (tfosEp) purified from recombinant Escherichia coli (Martel et al. [2010]) resulted in the complete hydrolysis of non-fermentable fructan moieties (Figure 1). The total monosaccharide (glucose and fructose) content of GJ + tfosEp (73.31 [±0.67] mg mL−1) was over two-fold higher than that of untreated GJ (30.39 [±1.51] mg mL−1); the sucrose content of both was negligible (0.60 [±0.06] mg mL−1). Grass juice contains smaller amounts of other sugars (e.g., galactooligosaccharides and maltosaccharides) in addition to proteins which can also be used for fermentation and growth; non-fermentable carbohydrates (i.e., lignin, cellulose and hemicellulose) are found in grass pulp and the fibrous biomass fraction (Charlton et al. [2009]). GJ + tfosEp was found to support optimal yeast growth, bioethanol and biomass production and was consequently employed as the feedstock for all experimental work reported in the present study.

Bottom Line: Maximum product yields for MYA-769 (39.18 [±2.42] mg ethanol mL(-1) and 4.96 [±0.15] g dry weight L(-1)) compared closely to those of Turbo (37.43 [±1.99] mg mL(-1) and 4.78 [±0.10] g L(-1), respectively).Co-production, marketing and/or on-site utilisation of probiotic yeast biomass as a direct-fed microbial to improve livestock health represents a novel and viable prospect for rural biorefineries.Given emergent evidence to suggest that dietary yeast supplementations might also mitigate ruminant enteric methane emissions, the administration of probiotic yeast biomass could also offer an economically feasible way of reducing atmospheric CH4.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Life Science, College of Medicine, Swansea University, Swansea SA2 8PP, Wales, UK.

ABSTRACT
Microbial biotechnology and biotransformations promise to diversify the scope of the biorefinery approach for the production of high-value products and biofuels from industrial, rural and municipal waste feedstocks. In addition to bio-based chemicals and metabolites, microbial biomass itself constitutes an obvious but overlooked by-product of existing biofermentation systems which warrants fuller attention. The probiotic yeast Saccharomyces boulardii is used to treat gastrointestinal disorders and marketed as a human health supplement. Despite its relatedness to S. cerevisiae that is employed widely in biotechnology, food and biofuel industries, the alternative applications of S. boulardii are not well studied. Using a biorefinery approach, we compared the bioethanol and biomass yields attainable from agriculturally-sourced grass juice using probiotic S. boulardii (strain MYA-769) and a commercial S. cerevisiae brewing strain (Turbo yeast). Maximum product yields for MYA-769 (39.18 [±2.42] mg ethanol mL(-1) and 4.96 [±0.15] g dry weight L(-1)) compared closely to those of Turbo (37.43 [±1.99] mg mL(-1) and 4.78 [±0.10] g L(-1), respectively). Co-production, marketing and/or on-site utilisation of probiotic yeast biomass as a direct-fed microbial to improve livestock health represents a novel and viable prospect for rural biorefineries. Given emergent evidence to suggest that dietary yeast supplementations might also mitigate ruminant enteric methane emissions, the administration of probiotic yeast biomass could also offer an economically feasible way of reducing atmospheric CH4.

No MeSH data available.


Related in: MedlinePlus