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New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus

Sinclair’s model fitted to ADE (1) and no-ADE (0) animals: the fitted parameters for ADE animalsM = 1.97 anda = 0.41 are very similar to those reported by Sinclair in(Sinclair and Senter1967).
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Fig6: Sinclair’s model fitted to ADE (1) and no-ADE (0) animals: the fitted parameters for ADE animalsM = 1.97 anda = 0.41 are very similar to those reported by Sinclair in(Sinclair and Senter1967).

Mentions: where d = {0, 1, … } is the amount of days after representation of alcohol and M and a are the model parameters to be fitted to the data. Parameter values are given that account for 98% of the daily variation (a = 0.40; M = 1.9 g/kg and M = 22 for net EtOH increase and preference respectively). Figure 6 depicts the curve 1.9e-0.4d (discontinuous (−−) line). In analogy to the approach of Sinclair et al. we computed the increased water-penalized EtOH intake of successive AD days with respect to baseline for ADE and non-ADE animals. Both groups were fitted to an exponential decay. The fitted parameters of the animals classified as presenting ADE (M = 1.97 g/kg and a = 0.41) agree very well with the values obtained by Sinclair et al. The fit for no-ADE classified animals, on the other hand, yields very different parameters (M = 0.59 and a = 0.61).Figure 6


New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

Sinclair’s model fitted to ADE (1) and no-ADE (0) animals: the fitted parameters for ADE animalsM = 1.97 anda = 0.41 are very similar to those reported by Sinclair in(Sinclair and Senter1967).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230819&req=5

Fig6: Sinclair’s model fitted to ADE (1) and no-ADE (0) animals: the fitted parameters for ADE animalsM = 1.97 anda = 0.41 are very similar to those reported by Sinclair in(Sinclair and Senter1967).
Mentions: where d = {0, 1, … } is the amount of days after representation of alcohol and M and a are the model parameters to be fitted to the data. Parameter values are given that account for 98% of the daily variation (a = 0.40; M = 1.9 g/kg and M = 22 for net EtOH increase and preference respectively). Figure 6 depicts the curve 1.9e-0.4d (discontinuous (−−) line). In analogy to the approach of Sinclair et al. we computed the increased water-penalized EtOH intake of successive AD days with respect to baseline for ADE and non-ADE animals. Both groups were fitted to an exponential decay. The fitted parameters of the animals classified as presenting ADE (M = 1.97 g/kg and a = 0.41) agree very well with the values obtained by Sinclair et al. The fit for no-ADE classified animals, on the other hand, yields very different parameters (M = 0.59 and a = 0.61).Figure 6

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus