Limits...
New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus

Ethanol intake in g/kg body weight (A and B) differentiates between the increase in the first group of animals (1st-5thAD phases) and the second group (9thAD phase). H2O penalized net EtOH intake (C and D), however, shows a similar intake for both controls and quinine animals from both data sets. P-values are computed from a two-sample t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4230819&req=5

Fig5: Ethanol intake in g/kg body weight (A and B) differentiates between the increase in the first group of animals (1st-5thAD phases) and the second group (9thAD phase). H2O penalized net EtOH intake (C and D), however, shows a similar intake for both controls and quinine animals from both data sets. P-values are computed from a two-sample t-test.

Mentions: To further validate the robustness of our intake measure we compared alcohol intake following a deprivation phase between two sets of animals. Data from 1st, 3rd, and 5th ADE stem from one group of animals. For comparison we use data from a 9th ADE of a different group of rats. We see that that intake in g/kg body weight differs considerably between the two groups (1st, 3rd, and 5th versus 9th ADE) (Figure 5A, B). The water penalized net EtOH intake, however, behaves similarly for both data sets (Figure 5C and D).Figure 5


New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

Ethanol intake in g/kg body weight (A and B) differentiates between the increase in the first group of animals (1st-5thAD phases) and the second group (9thAD phase). H2O penalized net EtOH intake (C and D), however, shows a similar intake for both controls and quinine animals from both data sets. P-values are computed from a two-sample t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230819&req=5

Fig5: Ethanol intake in g/kg body weight (A and B) differentiates between the increase in the first group of animals (1st-5thAD phases) and the second group (9thAD phase). H2O penalized net EtOH intake (C and D), however, shows a similar intake for both controls and quinine animals from both data sets. P-values are computed from a two-sample t-test.
Mentions: To further validate the robustness of our intake measure we compared alcohol intake following a deprivation phase between two sets of animals. Data from 1st, 3rd, and 5th ADE stem from one group of animals. For comparison we use data from a 9th ADE of a different group of rats. We see that that intake in g/kg body weight differs considerably between the two groups (1st, 3rd, and 5th versus 9th ADE) (Figure 5A, B). The water penalized net EtOH intake, however, behaves similarly for both data sets (Figure 5C and D).Figure 5

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus