Limits...
New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus

ADE classification: Mean H2O-penalized net EtOH increase of the first 2 AD days (y-axis) as a function of the H2O-penalized EtOH intake during baseline drinking (x-axis). No dependence of the increase in alcohol intake after deprivation on the alcohol intake during the preceding baseline can be observed, so the increase can be modeled as a normally distributed random variable. A normal distribution (whose mean is depicted as a solid line) is fitted to the controls, and the likelihood of all animals given the fitted parameters is computed. Animals presenting ADE are selected to have likelihood greater than a threshold value (circles).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4230819&req=5

Fig3: ADE classification: Mean H2O-penalized net EtOH increase of the first 2 AD days (y-axis) as a function of the H2O-penalized EtOH intake during baseline drinking (x-axis). No dependence of the increase in alcohol intake after deprivation on the alcohol intake during the preceding baseline can be observed, so the increase can be modeled as a normally distributed random variable. A normal distribution (whose mean is depicted as a solid line) is fitted to the controls, and the likelihood of all animals given the fitted parameters is computed. Animals presenting ADE are selected to have likelihood greater than a threshold value (circles).

Mentions: Note that Ir can be modeled as a normally distributed random variable because the increase in consumed alcohol intake after a deprivation phase does not depend on the amount of consumed alcohol during the preceding baseline phase (Figure 3).Figure 3


New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

ADE classification: Mean H2O-penalized net EtOH increase of the first 2 AD days (y-axis) as a function of the H2O-penalized EtOH intake during baseline drinking (x-axis). No dependence of the increase in alcohol intake after deprivation on the alcohol intake during the preceding baseline can be observed, so the increase can be modeled as a normally distributed random variable. A normal distribution (whose mean is depicted as a solid line) is fitted to the controls, and the likelihood of all animals given the fitted parameters is computed. Animals presenting ADE are selected to have likelihood greater than a threshold value (circles).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230819&req=5

Fig3: ADE classification: Mean H2O-penalized net EtOH increase of the first 2 AD days (y-axis) as a function of the H2O-penalized EtOH intake during baseline drinking (x-axis). No dependence of the increase in alcohol intake after deprivation on the alcohol intake during the preceding baseline can be observed, so the increase can be modeled as a normally distributed random variable. A normal distribution (whose mean is depicted as a solid line) is fitted to the controls, and the likelihood of all animals given the fitted parameters is computed. Animals presenting ADE are selected to have likelihood greater than a threshold value (circles).
Mentions: Note that Ir can be modeled as a normally distributed random variable because the increase in consumed alcohol intake after a deprivation phase does not depend on the amount of consumed alcohol during the preceding baseline phase (Figure 3).Figure 3

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus