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New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus

Graphic representation of standard measures (A,B,C) for alcohol intake and the H2O penalized net EtOH (D) shown for different baseline phases (B1, B3, and B5). Consumed amount (A) and preference (B) give a general overview of the intake but do not provide a measure of the net ethanol consumption. The classical measure of ethanol intake (C) is modified to include information on the additional consumption of water (water-penalized net EtOH intake (D)).
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Fig1: Graphic representation of standard measures (A,B,C) for alcohol intake and the H2O penalized net EtOH (D) shown for different baseline phases (B1, B3, and B5). Consumed amount (A) and preference (B) give a general overview of the intake but do not provide a measure of the net ethanol consumption. The classical measure of ethanol intake (C) is modified to include information on the additional consumption of water (water-penalized net EtOH intake (D)).

Mentions: The ethanol preference is proportional to the daily consumed amount of each solution, and for baseline drinking usually decreases with increasing alcohol concentrations (see data from baseline 1, 3, and 5 shown in Figure 1A and B). The preference measure gives a general overview over the drinking behavior, but provides no information on the net alcohol intake. For these measures the drinking profile differs from the ethanol preference profile (Figure 1C and D). While the intake profiles described by ethanol intake in g/kg body weight and water penalized net EtOH intake look very similar, the overall ethanol intake tends to decrease with subsequent baseline phases (Figure 2A), since the animals gain weight and reduce water consumption. The water-penalized net EtOH intake is stable throughout the consecutive baseline phases (Figure 2B).Figure 1


New measurement criteria for studying alcohol drinking and relapse in rodents.

Villarín Pildaín L, Vengeliene V, Matthäus F - In Silico Pharmacol (2013)

Graphic representation of standard measures (A,B,C) for alcohol intake and the H2O penalized net EtOH (D) shown for different baseline phases (B1, B3, and B5). Consumed amount (A) and preference (B) give a general overview of the intake but do not provide a measure of the net ethanol consumption. The classical measure of ethanol intake (C) is modified to include information on the additional consumption of water (water-penalized net EtOH intake (D)).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230819&req=5

Fig1: Graphic representation of standard measures (A,B,C) for alcohol intake and the H2O penalized net EtOH (D) shown for different baseline phases (B1, B3, and B5). Consumed amount (A) and preference (B) give a general overview of the intake but do not provide a measure of the net ethanol consumption. The classical measure of ethanol intake (C) is modified to include information on the additional consumption of water (water-penalized net EtOH intake (D)).
Mentions: The ethanol preference is proportional to the daily consumed amount of each solution, and for baseline drinking usually decreases with increasing alcohol concentrations (see data from baseline 1, 3, and 5 shown in Figure 1A and B). The preference measure gives a general overview over the drinking behavior, but provides no information on the net alcohol intake. For these measures the drinking profile differs from the ethanol preference profile (Figure 1C and D). While the intake profiles described by ethanol intake in g/kg body weight and water penalized net EtOH intake look very similar, the overall ethanol intake tends to decrease with subsequent baseline phases (Figure 2A), since the animals gain weight and reduce water consumption. The water-penalized net EtOH intake is stable throughout the consecutive baseline phases (Figure 2B).Figure 1

Bottom Line: This behaviour is called the alcohol deprivation effect (ADE).Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats.A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

View Article: PubMed Central - PubMed

Affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.

ABSTRACT

Purpose: Relapse to alcohol use is considered as one of the central features distinguishing dependence from controlled alcohol consumption. Relapse-like drinking in rodents is a transient episode of heavy drinking that follows a period of abstinence. This behaviour is called the alcohol deprivation effect (ADE). Not all animals develop behavioural changes that resemble relapse-like drinking behaviour. The purpose of our study was to develop a generalized quantitative criterion by which animals could be separated into two groups depending on their behaviour during a relapse-like situation (ADE vs. no-ADE).

Methods: An automated drinkometer system was used for data collection. This system measures fluid consumption by means of high-precision sensors attached to the drinking bottles in the home cage of the rat. We used a four bottle free choice paradigm with water 5, 10, and 20% ethanol solutions. For data analysis we developed a new measure of alcohol intake that quantifies net alcohol intake in relation to net consumption of water. This new measure is called water-penalized net ethanol intake.

Results: The new measure is more robust than commonly used measurements, such as alcohol preference and intake. It allows the comparison of alcohol intake between different groups of animals and different setups using an arbitrary number of bottles. Based on this new measure we developed a method to automatically select the threshold for the presence of ADE in individual animals.

Conclusions: Separating animals by their behavior during relapse-like situation could be used as one of the criteria for identification of alcohol addicted and non-addicted rats. A classification into presenting ADE or not is also essential to test the effectiveness of newly developed therapeutic drugs.

No MeSH data available.


Related in: MedlinePlus