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Trametes meyenii possesses elevated dye degradation abilities under normal nutritional conditions compared to other white rot fungi.

Chenaux PR, Lalji N, Lefebvre DD - AMB Express (2014)

Bottom Line: Trametes pubescens, T. multicolor, T. meyenii and T. versicolor decoloured amaranth azo-dye best on low-nitrogen agar-solidified media whereas Bjerkandera adusta and Phlebia radiata were most effective in low nitrogen medium supplemented with manganese.This appears to be because of inadequate Mn(3+) chelation required by manganese peroxidase because adding relatively low amounts of malonate enhanced decolouration rates.The ability of Trametes meyenii to simultaneously decolour dye over prolonged periods of time while growing in relatively nutrient-rich medium appears to be unique amongst white-rot fungi, indicating its potential in wastewater bioremediation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biology, Queen's University, Kingston K7L 3 N6, ON, Canada.

ABSTRACT
Several species of white-rot fungi were investigated for their utility in prolonged decolouration of the recalcitrant sulfonated azo dye, amaranth. Trametes pubescens, T. multicolor, T. meyenii and T. versicolor decoloured amaranth azo-dye best on low-nitrogen agar-solidified media whereas Bjerkandera adusta and Phlebia radiata were most effective in low nitrogen medium supplemented with manganese. Trametes cotonea did not decolour effectively under any condition. The decolouring Trametes species were also effective in liquid culture whereas B. adusta and P. radiata were not. Trametes meyenii, T. pubescens and T. multicolor were equal to or better than commonly employed T. versicolor at decolouring amaranth. This is the first study to show the dye decolouration potential of T. meyenii, T. pubescens, and T. multicolor. Supplementing with Mn(II) increased assayable manganese peroxidase activity, but not long-term decolouration, indicating that laccase is the main decolourizing enzyme in these Trametes species. This appears to be because of inadequate Mn(3+) chelation required by manganese peroxidase because adding relatively low amounts of malonate enhanced decolouration rates. The ability of Trametes meyenii to simultaneously decolour dye over prolonged periods of time while growing in relatively nutrient-rich medium appears to be unique amongst white-rot fungi, indicating its potential in wastewater bioremediation.

No MeSH data available.


Related in: MedlinePlus

Rate of amaranth decolouration in the presence and absence of malonate by fourTrametesspecies grown in low-N Kirk’s. Presence and absence of 50 μM sodium malonate. All values are means ± S.D. (n = 5).
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Figure 3: Rate of amaranth decolouration in the presence and absence of malonate by fourTrametesspecies grown in low-N Kirk’s. Presence and absence of 50 μM sodium malonate. All values are means ± S.D. (n = 5).

Mentions: Rates of decoloration increased significantly for all species of Trametes when 50 μM sodium malonate was added to cultures that had been pretreated in low-N Kirk’s (Figure 3). However, a comparison between species found no significant differences among the resultant rates of decoloration on a per fresh wt basis.


Trametes meyenii possesses elevated dye degradation abilities under normal nutritional conditions compared to other white rot fungi.

Chenaux PR, Lalji N, Lefebvre DD - AMB Express (2014)

Rate of amaranth decolouration in the presence and absence of malonate by fourTrametesspecies grown in low-N Kirk’s. Presence and absence of 50 μM sodium malonate. All values are means ± S.D. (n = 5).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230814&req=5

Figure 3: Rate of amaranth decolouration in the presence and absence of malonate by fourTrametesspecies grown in low-N Kirk’s. Presence and absence of 50 μM sodium malonate. All values are means ± S.D. (n = 5).
Mentions: Rates of decoloration increased significantly for all species of Trametes when 50 μM sodium malonate was added to cultures that had been pretreated in low-N Kirk’s (Figure 3). However, a comparison between species found no significant differences among the resultant rates of decoloration on a per fresh wt basis.

Bottom Line: Trametes pubescens, T. multicolor, T. meyenii and T. versicolor decoloured amaranth azo-dye best on low-nitrogen agar-solidified media whereas Bjerkandera adusta and Phlebia radiata were most effective in low nitrogen medium supplemented with manganese.This appears to be because of inadequate Mn(3+) chelation required by manganese peroxidase because adding relatively low amounts of malonate enhanced decolouration rates.The ability of Trametes meyenii to simultaneously decolour dye over prolonged periods of time while growing in relatively nutrient-rich medium appears to be unique amongst white-rot fungi, indicating its potential in wastewater bioremediation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biology, Queen's University, Kingston K7L 3 N6, ON, Canada.

ABSTRACT
Several species of white-rot fungi were investigated for their utility in prolonged decolouration of the recalcitrant sulfonated azo dye, amaranth. Trametes pubescens, T. multicolor, T. meyenii and T. versicolor decoloured amaranth azo-dye best on low-nitrogen agar-solidified media whereas Bjerkandera adusta and Phlebia radiata were most effective in low nitrogen medium supplemented with manganese. Trametes cotonea did not decolour effectively under any condition. The decolouring Trametes species were also effective in liquid culture whereas B. adusta and P. radiata were not. Trametes meyenii, T. pubescens and T. multicolor were equal to or better than commonly employed T. versicolor at decolouring amaranth. This is the first study to show the dye decolouration potential of T. meyenii, T. pubescens, and T. multicolor. Supplementing with Mn(II) increased assayable manganese peroxidase activity, but not long-term decolouration, indicating that laccase is the main decolourizing enzyme in these Trametes species. This appears to be because of inadequate Mn(3+) chelation required by manganese peroxidase because adding relatively low amounts of malonate enhanced decolouration rates. The ability of Trametes meyenii to simultaneously decolour dye over prolonged periods of time while growing in relatively nutrient-rich medium appears to be unique amongst white-rot fungi, indicating its potential in wastewater bioremediation.

No MeSH data available.


Related in: MedlinePlus