Limits...
Cultivation and detection of endophytic aerobic methanotrophs isolated from Sphagnum species as a perspective for environmental biotechnology.

Stępniewska Z, Kuźniar A - AMB Express (2014)

Bottom Line: During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product.It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria.Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynow 1I, Lublin, 20-708, Poland.

ABSTRACT
Enriched cultures of microorganisms are an essential step in the production of inoculum of these organisms for biotechnology and bioengineering. The potential application of methanotrophic microorganisms for removal of methane produced from landfills and coal mines as well as biodegradation of toxic compounds has been widely studied. Therefore, searching for new sources of methanotrophs can contribute to increasing the possibilities of biotechnology and bioengineering. Enrichment cultures of endophytic methanotrophs from Sphagnum sp. were initiated in NMS medium, a most widely used medium for cultivation of methanotrophic bacteria from various environments proposed in 1970 by Whittenbury. Incubation was carried out at 10, 20, 30, and 37°C with vigorous shaking on a shaker (180 rpm). The source of carbon and energy for endophytes were methane at the concentration range between 1-20%. It appeared that the consortium of endophytic bacteria grew only at the temperature of 20 and 30°C. During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product. The use of FISH has made characterization of endophytic consortia possible. It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria. Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

No MeSH data available.


Related in: MedlinePlus

Methanotrophic activity (MA) of the endophytic metanotrophic population obtained from leaves ofSphagnumsp gametophyte determined at 20oC (A) and 30oC (B) under different concentrations of methane (1- 20%). I – S. flexuosum M1, II – S. magellanicum M2, III – S. fallax M3, IV – S. magellanicum M4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4230809&req=5

Figure 3: Methanotrophic activity (MA) of the endophytic metanotrophic population obtained from leaves ofSphagnumsp gametophyte determined at 20oC (A) and 30oC (B) under different concentrations of methane (1- 20%). I – S. flexuosum M1, II – S. magellanicum M2, III – S. fallax M3, IV – S. magellanicum M4.

Mentions: The CH4 consumption in the enrichment culture (at 20 and 30°C) from the four localizations of Sphagnum sp. showed a clear decrease in the CH4 concentration tested in the headspace after 12–13 days of incubation (Figure 1). The highest CH4 consumption, below 20% of the initial CH4 concentration, was found in the isolated population from Sphagnum magellanicum (4,7 μMCH4 per ml liquid culture per day, Figure 3) at 30°C. No significant correlation in methanotrophic activity between the 20 and 30°C temperatures (UMW, p=0.89) was observed and no statistically significant differences between the activity of methanotrophic bacteria from the different species of Sphagnum sp. (UMW, p=0.85) were confirmed. A linear relationship with a high correlation coefficient (R2=0.99) between the methanotrophic activity of Sphagnum sp. endophytes and the methane concentration (in the range of CH4 from 0 to 10%, Table 1) was shown. The higher concentration of methane (over 10%) did not increase the methanotrophic activity of endophytes isolated from Sphagnum sp.. Furthermore, at a concentration of 20% CH4 it was observed to be even slightly lower, as in the case of the S. magellanicum M2 endophytes. The results obtained suggest that at 10% of methane complete saturation of the methane monooxygenase active center took place.


Cultivation and detection of endophytic aerobic methanotrophs isolated from Sphagnum species as a perspective for environmental biotechnology.

Stępniewska Z, Kuźniar A - AMB Express (2014)

Methanotrophic activity (MA) of the endophytic metanotrophic population obtained from leaves ofSphagnumsp gametophyte determined at 20oC (A) and 30oC (B) under different concentrations of methane (1- 20%). I – S. flexuosum M1, II – S. magellanicum M2, III – S. fallax M3, IV – S. magellanicum M4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230809&req=5

Figure 3: Methanotrophic activity (MA) of the endophytic metanotrophic population obtained from leaves ofSphagnumsp gametophyte determined at 20oC (A) and 30oC (B) under different concentrations of methane (1- 20%). I – S. flexuosum M1, II – S. magellanicum M2, III – S. fallax M3, IV – S. magellanicum M4.
Mentions: The CH4 consumption in the enrichment culture (at 20 and 30°C) from the four localizations of Sphagnum sp. showed a clear decrease in the CH4 concentration tested in the headspace after 12–13 days of incubation (Figure 1). The highest CH4 consumption, below 20% of the initial CH4 concentration, was found in the isolated population from Sphagnum magellanicum (4,7 μMCH4 per ml liquid culture per day, Figure 3) at 30°C. No significant correlation in methanotrophic activity between the 20 and 30°C temperatures (UMW, p=0.89) was observed and no statistically significant differences between the activity of methanotrophic bacteria from the different species of Sphagnum sp. (UMW, p=0.85) were confirmed. A linear relationship with a high correlation coefficient (R2=0.99) between the methanotrophic activity of Sphagnum sp. endophytes and the methane concentration (in the range of CH4 from 0 to 10%, Table 1) was shown. The higher concentration of methane (over 10%) did not increase the methanotrophic activity of endophytes isolated from Sphagnum sp.. Furthermore, at a concentration of 20% CH4 it was observed to be even slightly lower, as in the case of the S. magellanicum M2 endophytes. The results obtained suggest that at 10% of methane complete saturation of the methane monooxygenase active center took place.

Bottom Line: During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product.It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria.Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynow 1I, Lublin, 20-708, Poland.

ABSTRACT
Enriched cultures of microorganisms are an essential step in the production of inoculum of these organisms for biotechnology and bioengineering. The potential application of methanotrophic microorganisms for removal of methane produced from landfills and coal mines as well as biodegradation of toxic compounds has been widely studied. Therefore, searching for new sources of methanotrophs can contribute to increasing the possibilities of biotechnology and bioengineering. Enrichment cultures of endophytic methanotrophs from Sphagnum sp. were initiated in NMS medium, a most widely used medium for cultivation of methanotrophic bacteria from various environments proposed in 1970 by Whittenbury. Incubation was carried out at 10, 20, 30, and 37°C with vigorous shaking on a shaker (180 rpm). The source of carbon and energy for endophytes were methane at the concentration range between 1-20%. It appeared that the consortium of endophytic bacteria grew only at the temperature of 20 and 30°C. During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product. The use of FISH has made characterization of endophytic consortia possible. It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria. Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

No MeSH data available.


Related in: MedlinePlus