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Cultivation and detection of endophytic aerobic methanotrophs isolated from Sphagnum species as a perspective for environmental biotechnology.

Stępniewska Z, Kuźniar A - AMB Express (2014)

Bottom Line: During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product.It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria.Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynow 1I, Lublin, 20-708, Poland.

ABSTRACT
Enriched cultures of microorganisms are an essential step in the production of inoculum of these organisms for biotechnology and bioengineering. The potential application of methanotrophic microorganisms for removal of methane produced from landfills and coal mines as well as biodegradation of toxic compounds has been widely studied. Therefore, searching for new sources of methanotrophs can contribute to increasing the possibilities of biotechnology and bioengineering. Enrichment cultures of endophytic methanotrophs from Sphagnum sp. were initiated in NMS medium, a most widely used medium for cultivation of methanotrophic bacteria from various environments proposed in 1970 by Whittenbury. Incubation was carried out at 10, 20, 30, and 37°C with vigorous shaking on a shaker (180 rpm). The source of carbon and energy for endophytes were methane at the concentration range between 1-20%. It appeared that the consortium of endophytic bacteria grew only at the temperature of 20 and 30°C. During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product. The use of FISH has made characterization of endophytic consortia possible. It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria. Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

No MeSH data available.


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The growth curves of methanotrophic consortium cultures (at different 1-20% concentrations of CH4) isolated from leaves ofSphagnumsp. gametophytes: SF1 –S. flexuosum, SM2–S. magellanicum2, SA3–S. fallax, SM4 –S. magellanicum4.
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Figure 2: The growth curves of methanotrophic consortium cultures (at different 1-20% concentrations of CH4) isolated from leaves ofSphagnumsp. gametophytes: SF1 –S. flexuosum, SM2–S. magellanicum2, SA3–S. fallax, SM4 –S. magellanicum4.

Mentions: During the initial phase, adaptation of endophytes ranged from 5 to 8 days at 30°C and up to 12 days at 20°C, which can be due to cellular metabolism at lower temperature (Figure 2). Then, there was rapid growth of the endophytic population (logarithmic phase), which was reflected by an increase in optical density in the range from 0.3 to 2.0 at 30°C and from 0.25 to 1.4 at 20°C, depending on the methane concentration (1-20%). After the logarithmic phase, lack of carbon/energy sources was observed and an increase in the concentration of waste products to a harmful level for methanotrophic consortia (about day 13, Figure 2). This time was referred to as the stationary phase of the enrichment cultures.


Cultivation and detection of endophytic aerobic methanotrophs isolated from Sphagnum species as a perspective for environmental biotechnology.

Stępniewska Z, Kuźniar A - AMB Express (2014)

The growth curves of methanotrophic consortium cultures (at different 1-20% concentrations of CH4) isolated from leaves ofSphagnumsp. gametophytes: SF1 –S. flexuosum, SM2–S. magellanicum2, SA3–S. fallax, SM4 –S. magellanicum4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230809&req=5

Figure 2: The growth curves of methanotrophic consortium cultures (at different 1-20% concentrations of CH4) isolated from leaves ofSphagnumsp. gametophytes: SF1 –S. flexuosum, SM2–S. magellanicum2, SA3–S. fallax, SM4 –S. magellanicum4.
Mentions: During the initial phase, adaptation of endophytes ranged from 5 to 8 days at 30°C and up to 12 days at 20°C, which can be due to cellular metabolism at lower temperature (Figure 2). Then, there was rapid growth of the endophytic population (logarithmic phase), which was reflected by an increase in optical density in the range from 0.3 to 2.0 at 30°C and from 0.25 to 1.4 at 20°C, depending on the methane concentration (1-20%). After the logarithmic phase, lack of carbon/energy sources was observed and an increase in the concentration of waste products to a harmful level for methanotrophic consortia (about day 13, Figure 2). This time was referred to as the stationary phase of the enrichment cultures.

Bottom Line: During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product.It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria.Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynow 1I, Lublin, 20-708, Poland.

ABSTRACT
Enriched cultures of microorganisms are an essential step in the production of inoculum of these organisms for biotechnology and bioengineering. The potential application of methanotrophic microorganisms for removal of methane produced from landfills and coal mines as well as biodegradation of toxic compounds has been widely studied. Therefore, searching for new sources of methanotrophs can contribute to increasing the possibilities of biotechnology and bioengineering. Enrichment cultures of endophytic methanotrophs from Sphagnum sp. were initiated in NMS medium, a most widely used medium for cultivation of methanotrophic bacteria from various environments proposed in 1970 by Whittenbury. Incubation was carried out at 10, 20, 30, and 37°C with vigorous shaking on a shaker (180 rpm). The source of carbon and energy for endophytes were methane at the concentration range between 1-20%. It appeared that the consortium of endophytic bacteria grew only at the temperature of 20 and 30°C. During the culture of endophytes, the measurements of gas concentration showed a steady loss of methane and oxygen, as well as accumulation of carbon dioxide as a CH4 oxidation product. The use of FISH has made characterization of endophytic consortia possible. It turned out that the population of endophytes consists of type I and II methanotrophs as well as associated non-methanotrophic bacteria. Furthermore, we determined the potential of the examined bacteria for methane oxidation, which ranged up to 4,7 μMCH4 per ml of the population of endophytes per day.

No MeSH data available.


Related in: MedlinePlus