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Evaluation of in vivo antitrypanosomal activity of crude extracts of Artemisia abyssinica against aTrypanosoma congolense isolate.

Feyera T, Terefe G, Shibeshi W - BMC Complement Altern Med (2014)

Bottom Line: The level of parasitaemia, body weight, packed cell volume, differential leukocyte counts and mean survival period were monitored.The study showed that the DCM extract at 200 and 400 mg/kg, and the hydromethanolic extract at 400 mg/kg reduced parasitaemia (p < 0.05), ameliorated anaemia (p < 0.05), prevented body weight loss (p < 0.05) and resulted in significant increase in neutrophil levels (p < 0.05) and marked decrease in lymphocyte levels (p < 0.05) compared to the negative control.This study established that aerial parts of A. abyssinica have antitrypanosomal potential and can be considered a potential source of new drugs for the treatment of tropical diseases caused by trypanosomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, P,O, Box 9086, Addis Ababa, Ethiopia. workineh.shibeshi@aau.edu.et.

ABSTRACT

Background: African trypanosomiasis is a major disease of economic and public health importance affecting agricultural and human development. The search for alternative compounds against African trypanosomiasis is justified by various limitations of existing chemotherapeutic agents. This study was aimed at screening the hydromethanolic and dichloromethane (DCM) crude extracts of aerial parts of Artemisia abyssinica for in vivo antitrypanosomal activity against Trypanosoma congolense isolate in mice.

Methods: The aerial parts of the plant were extracted by maceration technique using dichloromethane and 80% methanol to obtain the corresponding crude extracts. The plant extracts at doses of 100, 200 and 400 mg/kg body weight were administered intraperitoneally daily for 7 days to mice infected with Trypanosoma congolense. Diminazene aceturate and distilled water were used as positive and as negative controls respectively. The level of parasitaemia, body weight, packed cell volume, differential leukocyte counts and mean survival period were monitored.

Results: The study showed that the DCM extract at 200 and 400 mg/kg, and the hydromethanolic extract at 400 mg/kg reduced parasitaemia (p < 0.05), ameliorated anaemia (p < 0.05), prevented body weight loss (p < 0.05) and resulted in significant increase in neutrophil levels (p < 0.05) and marked decrease in lymphocyte levels (p < 0.05) compared to the negative control.

Conclusions: This study established that aerial parts of A. abyssinica have antitrypanosomal potential and can be considered a potential source of new drugs for the treatment of tropical diseases caused by trypanosomes.

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Related in: MedlinePlus

Effect of extract of Artemisia abyssinica on differential white blood cell count of mice. (a) hydromethanolic crude extract (b) dichloromethane crude extract. Values are mean ± SEM; n = 6; UU = uninfected-untreated; WBC = white blood cell; a = p < 0.05 compared to negative control; b = p < 0.05 compared to uninfected-untreated.
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Figure 3: Effect of extract of Artemisia abyssinica on differential white blood cell count of mice. (a) hydromethanolic crude extract (b) dichloromethane crude extract. Values are mean ± SEM; n = 6; UU = uninfected-untreated; WBC = white blood cell; a = p < 0.05 compared to negative control; b = p < 0.05 compared to uninfected-untreated.

Mentions: The results in Figure 3a and b indicate that differential white blood cell (WBC) count revealed that% lymphocyte counts were significantly higher (p < 0.05) in the groups that received lowest dose (100 mg/kg) of both extracts, 3.5 mg/kg DA and in the infected-untreated group compared to uninfected-untreated group; whereas marked decrease (p < 0.05) in neutrophil count was noticed in these groups.


Evaluation of in vivo antitrypanosomal activity of crude extracts of Artemisia abyssinica against aTrypanosoma congolense isolate.

Feyera T, Terefe G, Shibeshi W - BMC Complement Altern Med (2014)

Effect of extract of Artemisia abyssinica on differential white blood cell count of mice. (a) hydromethanolic crude extract (b) dichloromethane crude extract. Values are mean ± SEM; n = 6; UU = uninfected-untreated; WBC = white blood cell; a = p < 0.05 compared to negative control; b = p < 0.05 compared to uninfected-untreated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4230807&req=5

Figure 3: Effect of extract of Artemisia abyssinica on differential white blood cell count of mice. (a) hydromethanolic crude extract (b) dichloromethane crude extract. Values are mean ± SEM; n = 6; UU = uninfected-untreated; WBC = white blood cell; a = p < 0.05 compared to negative control; b = p < 0.05 compared to uninfected-untreated.
Mentions: The results in Figure 3a and b indicate that differential white blood cell (WBC) count revealed that% lymphocyte counts were significantly higher (p < 0.05) in the groups that received lowest dose (100 mg/kg) of both extracts, 3.5 mg/kg DA and in the infected-untreated group compared to uninfected-untreated group; whereas marked decrease (p < 0.05) in neutrophil count was noticed in these groups.

Bottom Line: The level of parasitaemia, body weight, packed cell volume, differential leukocyte counts and mean survival period were monitored.The study showed that the DCM extract at 200 and 400 mg/kg, and the hydromethanolic extract at 400 mg/kg reduced parasitaemia (p < 0.05), ameliorated anaemia (p < 0.05), prevented body weight loss (p < 0.05) and resulted in significant increase in neutrophil levels (p < 0.05) and marked decrease in lymphocyte levels (p < 0.05) compared to the negative control.This study established that aerial parts of A. abyssinica have antitrypanosomal potential and can be considered a potential source of new drugs for the treatment of tropical diseases caused by trypanosomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, P,O, Box 9086, Addis Ababa, Ethiopia. workineh.shibeshi@aau.edu.et.

ABSTRACT

Background: African trypanosomiasis is a major disease of economic and public health importance affecting agricultural and human development. The search for alternative compounds against African trypanosomiasis is justified by various limitations of existing chemotherapeutic agents. This study was aimed at screening the hydromethanolic and dichloromethane (DCM) crude extracts of aerial parts of Artemisia abyssinica for in vivo antitrypanosomal activity against Trypanosoma congolense isolate in mice.

Methods: The aerial parts of the plant were extracted by maceration technique using dichloromethane and 80% methanol to obtain the corresponding crude extracts. The plant extracts at doses of 100, 200 and 400 mg/kg body weight were administered intraperitoneally daily for 7 days to mice infected with Trypanosoma congolense. Diminazene aceturate and distilled water were used as positive and as negative controls respectively. The level of parasitaemia, body weight, packed cell volume, differential leukocyte counts and mean survival period were monitored.

Results: The study showed that the DCM extract at 200 and 400 mg/kg, and the hydromethanolic extract at 400 mg/kg reduced parasitaemia (p < 0.05), ameliorated anaemia (p < 0.05), prevented body weight loss (p < 0.05) and resulted in significant increase in neutrophil levels (p < 0.05) and marked decrease in lymphocyte levels (p < 0.05) compared to the negative control.

Conclusions: This study established that aerial parts of A. abyssinica have antitrypanosomal potential and can be considered a potential source of new drugs for the treatment of tropical diseases caused by trypanosomes.

Show MeSH
Related in: MedlinePlus