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Influence of endogamy and mitochondrial DNA on immunological parameters in cattle.

Macedo AA, Bittar JF, Bassi PB, Ronda JB, Bittar ER, Panetto JC, Araujo MS, Santos RL, Martins-Filho OA - BMC Vet. Res. (2014)

Bottom Line: However, the effect of endogamy and mitochondrial DNA (mtDNA) on the immune system remains unclear.Multiple regression analysis indicated that endogamy in calves was associated with increased CD8+ T and CD21+ B lymphocytes, and decreased γδhigh T cells in peripheral blood.These results demonstrated for the first time that endogamy influences the immune system of cattle.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. rsantos@vet.ufmg.br.

ABSTRACT

Background: Endogamy increases the risk of manifestation of deleterious recessive genes. Mitochondrial DNA allows the separation of American Zebu (Bos indicus and Bos taurus) and evaluate the effect of mitochondrial DNA on productive traits of cattle. However, the effect of endogamy and mitochondrial DNA (mtDNA) on the immune system remains unclear. The aim of this study was to evaluate the association between endogamy, mtDNA and immune parameters.

Results: A total of 86 cattle (43 cows and 43 calves) were used in this study. Age, endogamy, milk yield, and origin of mtDNA were measured and their influence on immunological parameters was evaluated. Older cows had increased CD4+ T cells, decreased CD21+ and γδhigh T cells as well as increased CD4+/CD8+ and T/B ratio. Multiple regression analysis indicated that endogamy in calves was associated with increased CD8+ T and CD21+ B lymphocytes, and decreased γδhigh T cells in peripheral blood. Cows with medium and lower endogamy had a lower percentage of B lymphocytes and γδlow T cells and cows with lower endogamy had higher levels of γδ T cells and γδhigh T cells, as well as the CD4+/CD48+ cell ratio. Calves with higher endogamy had higher levels of CD8+ T lymphocytes, whereas calves with lower endogamy had lower levels of γδlow T cells.

Conclusions: These results demonstrated for the first time that endogamy influences the immune system of cattle.

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Related in: MedlinePlus

Percentage of lymphocytes in peripheral blood from Guzerat calves with high endogamy (n = 15), medium endogamy (n = 14) or low endogamy (n = 14) analyzed by flow cytometry. (A) CD4+ T lymphocytes, (B) CD8+ T lymphocytes, (C) B lymphocytes, (D) γδ+ T lymphocytes, (E) γδhigh T lymphocytes, (F) γδlow T lymphocytes, (G) CD4+/CD8+ cell ratio and (H) T/B cell ratio. Data represent mean and standard error. Statistically significant differences are indicated by asterisks (* p<0.05).
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Figure 2: Percentage of lymphocytes in peripheral blood from Guzerat calves with high endogamy (n = 15), medium endogamy (n = 14) or low endogamy (n = 14) analyzed by flow cytometry. (A) CD4+ T lymphocytes, (B) CD8+ T lymphocytes, (C) B lymphocytes, (D) γδ+ T lymphocytes, (E) γδhigh T lymphocytes, (F) γδlow T lymphocytes, (G) CD4+/CD8+ cell ratio and (H) T/B cell ratio. Data represent mean and standard error. Statistically significant differences are indicated by asterisks (* p<0.05).

Mentions: Based on endogamy coefficients, cows and calves were grouped into high, medium, and low endogamy as described in Methods. The immune profiles of these cattle were then analyzed by flow cytometry (Figures 1 and 2). These results indicated that cows with medium and low endogamy had a decreased percentage of B lymphocytes when compared to cows with high endogamy (p < 0.05; Figure 1C). Furthermore, cows with lower endogamy had a higher percentage of γδlow T cells when compared with cows with medium endogamy (p < 0.05; Figure 1F). There was also a clear influence of endogamy on the immunological profile of calves. Calves with high endogamy had an increased percentage of CD8+ T lymphocytes when compared to calves with medium and low endogamy (p < 0.05, Figure 2B). In contrast, calves with low endogamy had an increased percentage of γδT cells (p < 0.05; Figure 2D) and γδhigh T cells (p < 0.05; Figure 2E) as well as the CD4+/CD8+ T cell ratio (p < 0.05; Figure 2G) and T/B ratio (p < 0.05; Figure 2H) when compared to calves with high endogamy (p < 0.05). Calves with low endogamy had a decreased percentage of γδlow T cells only in comparison to those with medium endogamy (p < 0.05; Figure 2F).


Influence of endogamy and mitochondrial DNA on immunological parameters in cattle.

Macedo AA, Bittar JF, Bassi PB, Ronda JB, Bittar ER, Panetto JC, Araujo MS, Santos RL, Martins-Filho OA - BMC Vet. Res. (2014)

Percentage of lymphocytes in peripheral blood from Guzerat calves with high endogamy (n = 15), medium endogamy (n = 14) or low endogamy (n = 14) analyzed by flow cytometry. (A) CD4+ T lymphocytes, (B) CD8+ T lymphocytes, (C) B lymphocytes, (D) γδ+ T lymphocytes, (E) γδhigh T lymphocytes, (F) γδlow T lymphocytes, (G) CD4+/CD8+ cell ratio and (H) T/B cell ratio. Data represent mean and standard error. Statistically significant differences are indicated by asterisks (* p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230805&req=5

Figure 2: Percentage of lymphocytes in peripheral blood from Guzerat calves with high endogamy (n = 15), medium endogamy (n = 14) or low endogamy (n = 14) analyzed by flow cytometry. (A) CD4+ T lymphocytes, (B) CD8+ T lymphocytes, (C) B lymphocytes, (D) γδ+ T lymphocytes, (E) γδhigh T lymphocytes, (F) γδlow T lymphocytes, (G) CD4+/CD8+ cell ratio and (H) T/B cell ratio. Data represent mean and standard error. Statistically significant differences are indicated by asterisks (* p<0.05).
Mentions: Based on endogamy coefficients, cows and calves were grouped into high, medium, and low endogamy as described in Methods. The immune profiles of these cattle were then analyzed by flow cytometry (Figures 1 and 2). These results indicated that cows with medium and low endogamy had a decreased percentage of B lymphocytes when compared to cows with high endogamy (p < 0.05; Figure 1C). Furthermore, cows with lower endogamy had a higher percentage of γδlow T cells when compared with cows with medium endogamy (p < 0.05; Figure 1F). There was also a clear influence of endogamy on the immunological profile of calves. Calves with high endogamy had an increased percentage of CD8+ T lymphocytes when compared to calves with medium and low endogamy (p < 0.05, Figure 2B). In contrast, calves with low endogamy had an increased percentage of γδT cells (p < 0.05; Figure 2D) and γδhigh T cells (p < 0.05; Figure 2E) as well as the CD4+/CD8+ T cell ratio (p < 0.05; Figure 2G) and T/B ratio (p < 0.05; Figure 2H) when compared to calves with high endogamy (p < 0.05). Calves with low endogamy had a decreased percentage of γδlow T cells only in comparison to those with medium endogamy (p < 0.05; Figure 2F).

Bottom Line: However, the effect of endogamy and mitochondrial DNA (mtDNA) on the immune system remains unclear.Multiple regression analysis indicated that endogamy in calves was associated with increased CD8+ T and CD21+ B lymphocytes, and decreased γδhigh T cells in peripheral blood.These results demonstrated for the first time that endogamy influences the immune system of cattle.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. rsantos@vet.ufmg.br.

ABSTRACT

Background: Endogamy increases the risk of manifestation of deleterious recessive genes. Mitochondrial DNA allows the separation of American Zebu (Bos indicus and Bos taurus) and evaluate the effect of mitochondrial DNA on productive traits of cattle. However, the effect of endogamy and mitochondrial DNA (mtDNA) on the immune system remains unclear. The aim of this study was to evaluate the association between endogamy, mtDNA and immune parameters.

Results: A total of 86 cattle (43 cows and 43 calves) were used in this study. Age, endogamy, milk yield, and origin of mtDNA were measured and their influence on immunological parameters was evaluated. Older cows had increased CD4+ T cells, decreased CD21+ and γδhigh T cells as well as increased CD4+/CD8+ and T/B ratio. Multiple regression analysis indicated that endogamy in calves was associated with increased CD8+ T and CD21+ B lymphocytes, and decreased γδhigh T cells in peripheral blood. Cows with medium and lower endogamy had a lower percentage of B lymphocytes and γδlow T cells and cows with lower endogamy had higher levels of γδ T cells and γδhigh T cells, as well as the CD4+/CD48+ cell ratio. Calves with higher endogamy had higher levels of CD8+ T lymphocytes, whereas calves with lower endogamy had lower levels of γδlow T cells.

Conclusions: These results demonstrated for the first time that endogamy influences the immune system of cattle.

Show MeSH
Related in: MedlinePlus