World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonisation Project: IV. Tissue collection, processing, and storage in endometriosis research.
Bottom Line: We developed "recommended standard" and "minimum required" SOPs for the collection, processing, and storage of ectopic and eutopic endometrium, peritoneum, and myometrium, and a biospecimen data collection form necessary for interpretation of sample-derived results.The procedures are also relevant to research into other gynecologic conditions involving endometrium, myometrium, and peritoneum.The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies.
Affiliation: Organ Systems, Department of Development and Regeneration, KU Leuven, Leuven, Belgium; Department of Obstetrics and Gynecology, Leuven University Fertility Center, University Hospital Leuven, Leuven, Belgium.Show MeSH
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Mentions: We conducted two workshops in March and July 2013, followed by several rounds of expert review, bringing together 54 leaders in endometriosis research and sample processing from 34 clinical/academic centers and three industry collaborators in 16 countries, to develop and reach consensus on evidence-based phenome collection and SOP guidelines (Fig. 1). During Workshop I and a subsequent consultation round, we identified 24 centers around the globe that collect tissues from endometriosis case and control subjects on a large scale (publication on >100 cases). We identified four tissue types (ectopic endometrium, eutopic endometrium, myometrium, and peritoneum) that were collected by the centers (Table 1); all provided SOPs for sample collection, processing, and storage.
Affiliation: Organ Systems, Department of Development and Regeneration, KU Leuven, Leuven, Belgium; Department of Obstetrics and Gynecology, Leuven University Fertility Center, University Hospital Leuven, Leuven, Belgium.