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Association between single-nucleotide polymorphisms and early spontaneous hepatitis B virus e antigen seroconversion in children.

Komatsu H, Murakami J, Inui A, Tsunoda T, Sogo T, Fujisawa T - BMC Res Notes (2014)

Bottom Line: Of 225 Japanese patients with chronic hepatitis B virus infection (male/female: 105/120, median age at initial visit: 6 years; range 0-44 years), 52 achieved spontaneous HBeAg seroconversion at the age of 10 years or younger (G1: early seroconversion group), and 57 did not achieve spontaneous HBeAg seroconversion under the age of 20 years (G2: late or no seroconversion group).The association study found a low p-value, but not significant, to have early HBeAg seroconversion in the dominant model for HLA-DPA1 (genotype TC + TT vs.Although the HLA-DPA1 SNP did not show a statistically significant association with early HBeAg seroconversion in this study, the HLA-DPA1 SNP might increase the likelihood of achieving early spontaneous HBeAg seroconversion in children.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Toho University, Sakura Medical Center, 564-1 Shimoshizu Sakura, Chiba 285-8741, Japan. haruki-komatsu@chive.ocn.ne.jp.

ABSTRACT

Background: The disease progression following hepatitis B virus (HBV) infection is associated with single-nucleotide polymorphisms (SNPs). However, the role of SNPs in chronic HBV infection in children remains unclear. Here, we investigate the association between SNPs and early spontaneous hepatitis B e antigen (HBeAg) seroconversion in children with chronic hepatitis B infection.

Methods: This was a retrospective cohort study. We genotyped seven SNPs in the following genes, interleukin (IL)-10 (rs1800871 and rs1800872), human leukocyte antigen (HLA)-DPA1 (rs3077), HLA-DPB1 (rs9277535), HLA-DQB2 (rs7453920), HLA-DQB1 (rs2856718), and IL28B (rs8099917), in patients with chronic HBV infection using PCR and sequencing. These variants were analyzed for an association with early HBeAg seroconversion in children.

Results: Of 225 Japanese patients with chronic hepatitis B virus infection (male/female: 105/120, median age at initial visit: 6 years; range 0-44 years), 52 achieved spontaneous HBeAg seroconversion at the age of 10 years or younger (G1: early seroconversion group), and 57 did not achieve spontaneous HBeAg seroconversion under the age of 20 years (G2: late or no seroconversion group). Of the seven SNPs, only the HLA-DPA1 SNP displayed a low p-value (P = 0.070), but not significant, to have early HBeAg seroconversion in the dominant model and in the allele model (P = 0.073) using the chi-square test. The association study found a low p-value, but not significant, to have early HBeAg seroconversion in the dominant model for HLA-DPA1 (genotype TC + TT vs. CC, P = 0.070, odds ratio: 2.016, 95% confidence interval: 0.940-4.323) using a logistic regression model.

Conclusion: Although the HLA-DPA1 SNP did not show a statistically significant association with early HBeAg seroconversion in this study, the HLA-DPA1 SNP might increase the likelihood of achieving early spontaneous HBeAg seroconversion in children.

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Classification of the subjects based on hepatitis B e antigen seroconversion.
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Fig1: Classification of the subjects based on hepatitis B e antigen seroconversion.

Mentions: Between 1981 and 2012, a total of 225 Japanese patients comprising children, adolescents, and mothers (male/female: 105/120, age: 0–44 years, median age: 6 years, follow-up period: 1–25 years, median: 8 years) with chronic HBV infection were followed in the Pediatric Departments of Eastern Yokohama Hospital and Tottori University Hospital. Of the 225 patients with chronic HBV infection, 115 achieved HBeAg seroconversion and 110 did not achieve HBeAg seroconversion during the follow-up period. Of the 115 patients with HBeAg seroconversion, 63 achieved HBeAg seroconversion at the age of 10 years or younger, 34 achieved HBeAg seroconversion between the ages of 11 and 19 years, and 18 achieved HBeAg seroconversion at the age of 20 years or older. Of the 110 patients without HBeAg seroconversion during the follow-up period, 67 were under the age of 20 years, and 43 were 20 years or older. Of the 63 patients who achieved HBeAg seroconversion at the age of 10 years or younger, 52 were available for evaluation and classified into the early seroconversion group (G1). Of the 18 patients who achieved HBeAg seroconversion at the age of 20 years or older, 16 were available for evaluation. Of the 43 patients without HBeAg seroconversion who were 20 years or older, 41 were available for evaluation. A total of 57 patients were classified into the late or no seroconversion group (G2). The classification scheme of the patients based on HBeAg seroconversion is shown in Figure 1.Figure 1


Association between single-nucleotide polymorphisms and early spontaneous hepatitis B virus e antigen seroconversion in children.

Komatsu H, Murakami J, Inui A, Tsunoda T, Sogo T, Fujisawa T - BMC Res Notes (2014)

Classification of the subjects based on hepatitis B e antigen seroconversion.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4230755&req=5

Fig1: Classification of the subjects based on hepatitis B e antigen seroconversion.
Mentions: Between 1981 and 2012, a total of 225 Japanese patients comprising children, adolescents, and mothers (male/female: 105/120, age: 0–44 years, median age: 6 years, follow-up period: 1–25 years, median: 8 years) with chronic HBV infection were followed in the Pediatric Departments of Eastern Yokohama Hospital and Tottori University Hospital. Of the 225 patients with chronic HBV infection, 115 achieved HBeAg seroconversion and 110 did not achieve HBeAg seroconversion during the follow-up period. Of the 115 patients with HBeAg seroconversion, 63 achieved HBeAg seroconversion at the age of 10 years or younger, 34 achieved HBeAg seroconversion between the ages of 11 and 19 years, and 18 achieved HBeAg seroconversion at the age of 20 years or older. Of the 110 patients without HBeAg seroconversion during the follow-up period, 67 were under the age of 20 years, and 43 were 20 years or older. Of the 63 patients who achieved HBeAg seroconversion at the age of 10 years or younger, 52 were available for evaluation and classified into the early seroconversion group (G1). Of the 18 patients who achieved HBeAg seroconversion at the age of 20 years or older, 16 were available for evaluation. Of the 43 patients without HBeAg seroconversion who were 20 years or older, 41 were available for evaluation. A total of 57 patients were classified into the late or no seroconversion group (G2). The classification scheme of the patients based on HBeAg seroconversion is shown in Figure 1.Figure 1

Bottom Line: Of 225 Japanese patients with chronic hepatitis B virus infection (male/female: 105/120, median age at initial visit: 6 years; range 0-44 years), 52 achieved spontaneous HBeAg seroconversion at the age of 10 years or younger (G1: early seroconversion group), and 57 did not achieve spontaneous HBeAg seroconversion under the age of 20 years (G2: late or no seroconversion group).The association study found a low p-value, but not significant, to have early HBeAg seroconversion in the dominant model for HLA-DPA1 (genotype TC + TT vs.Although the HLA-DPA1 SNP did not show a statistically significant association with early HBeAg seroconversion in this study, the HLA-DPA1 SNP might increase the likelihood of achieving early spontaneous HBeAg seroconversion in children.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Toho University, Sakura Medical Center, 564-1 Shimoshizu Sakura, Chiba 285-8741, Japan. haruki-komatsu@chive.ocn.ne.jp.

ABSTRACT

Background: The disease progression following hepatitis B virus (HBV) infection is associated with single-nucleotide polymorphisms (SNPs). However, the role of SNPs in chronic HBV infection in children remains unclear. Here, we investigate the association between SNPs and early spontaneous hepatitis B e antigen (HBeAg) seroconversion in children with chronic hepatitis B infection.

Methods: This was a retrospective cohort study. We genotyped seven SNPs in the following genes, interleukin (IL)-10 (rs1800871 and rs1800872), human leukocyte antigen (HLA)-DPA1 (rs3077), HLA-DPB1 (rs9277535), HLA-DQB2 (rs7453920), HLA-DQB1 (rs2856718), and IL28B (rs8099917), in patients with chronic HBV infection using PCR and sequencing. These variants were analyzed for an association with early HBeAg seroconversion in children.

Results: Of 225 Japanese patients with chronic hepatitis B virus infection (male/female: 105/120, median age at initial visit: 6 years; range 0-44 years), 52 achieved spontaneous HBeAg seroconversion at the age of 10 years or younger (G1: early seroconversion group), and 57 did not achieve spontaneous HBeAg seroconversion under the age of 20 years (G2: late or no seroconversion group). Of the seven SNPs, only the HLA-DPA1 SNP displayed a low p-value (P = 0.070), but not significant, to have early HBeAg seroconversion in the dominant model and in the allele model (P = 0.073) using the chi-square test. The association study found a low p-value, but not significant, to have early HBeAg seroconversion in the dominant model for HLA-DPA1 (genotype TC + TT vs. CC, P = 0.070, odds ratio: 2.016, 95% confidence interval: 0.940-4.323) using a logistic regression model.

Conclusion: Although the HLA-DPA1 SNP did not show a statistically significant association with early HBeAg seroconversion in this study, the HLA-DPA1 SNP might increase the likelihood of achieving early spontaneous HBeAg seroconversion in children.

Show MeSH
Related in: MedlinePlus