Limits...
Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus

Lipid characterization of Lig 290. Lipid were extracted and derivatized to fatty acid methyl esters (FAME) and characterized via gas chromatography. The FAME speciesare consistent with a desirable biodiesel profile, categorized as C16 (C16:0–2), C18(C18:0–2), and C20 (which were not present). Omega-3′s contain C16 or C18 species. All other FAMEs were classified as non-biodiesel. Results presented are mean values (n = 3) and error bars are the standard error of the mean.
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Figure 5: Lipid characterization of Lig 290. Lipid were extracted and derivatized to fatty acid methyl esters (FAME) and characterized via gas chromatography. The FAME speciesare consistent with a desirable biodiesel profile, categorized as C16 (C16:0–2), C18(C18:0–2), and C20 (which were not present). Omega-3′s contain C16 or C18 species. All other FAMEs were classified as non-biodiesel. Results presented are mean values (n = 3) and error bars are the standard error of the mean.

Mentions: In order to confirm that the lipids produced by Lig 290 are appropriate for use as biodiesel, we performed a direct transesterification reaction. Upon evaporating excess organic solvent, a methanol-based transesterification was performed to convert Lig 290 isolated fatty acids to fatty acid methyl esters (FAME). FAMEs were then analyzed using gas chromatography, equipped with a flame ionization detector. Manual integration of the peak areas indicated that culturing at a pH 3 resulted in over 90% of the FAMEs produced by Lig 290 classified as high quality biodiesel (C16 – C18) with a single species of C16 FAME (C16:0) and multiple species of C18 (Figure 5). Additionally, the low pH condition also resulted in a dramatic increase in the amount of C16:0 FAME content. These results suggest that lower pH induces a shift in the FAME profile of Lig 290 towards biofuel compatible lipids. In addition to FAMEs, Lig 290 also produced a number of polyunsaturated fatty acids.


Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Lipid characterization of Lig 290. Lipid were extracted and derivatized to fatty acid methyl esters (FAME) and characterized via gas chromatography. The FAME speciesare consistent with a desirable biodiesel profile, categorized as C16 (C16:0–2), C18(C18:0–2), and C20 (which were not present). Omega-3′s contain C16 or C18 species. All other FAMEs were classified as non-biodiesel. Results presented are mean values (n = 3) and error bars are the standard error of the mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230719&req=5

Figure 5: Lipid characterization of Lig 290. Lipid were extracted and derivatized to fatty acid methyl esters (FAME) and characterized via gas chromatography. The FAME speciesare consistent with a desirable biodiesel profile, categorized as C16 (C16:0–2), C18(C18:0–2), and C20 (which were not present). Omega-3′s contain C16 or C18 species. All other FAMEs were classified as non-biodiesel. Results presented are mean values (n = 3) and error bars are the standard error of the mean.
Mentions: In order to confirm that the lipids produced by Lig 290 are appropriate for use as biodiesel, we performed a direct transesterification reaction. Upon evaporating excess organic solvent, a methanol-based transesterification was performed to convert Lig 290 isolated fatty acids to fatty acid methyl esters (FAME). FAMEs were then analyzed using gas chromatography, equipped with a flame ionization detector. Manual integration of the peak areas indicated that culturing at a pH 3 resulted in over 90% of the FAMEs produced by Lig 290 classified as high quality biodiesel (C16 – C18) with a single species of C16 FAME (C16:0) and multiple species of C18 (Figure 5). Additionally, the low pH condition also resulted in a dramatic increase in the amount of C16:0 FAME content. These results suggest that lower pH induces a shift in the FAME profile of Lig 290 towards biofuel compatible lipids. In addition to FAMEs, Lig 290 also produced a number of polyunsaturated fatty acids.

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus