Limits...
Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus

Lig 290 is identified as a robust high-lipid- producing Scenedesmus spp. a) When cultured at neutral pH the cells proliferate and maintain a green pigmentation. When cultured under low pH conditions (pH = 3), the microalgae take on a yellow/gold pigmentation. b) Gravimetric lipid analysis demonstrates increased in lipid content when cultured at pH = 3 over the course of 7–28 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4230719&req=5

Figure 4: Lig 290 is identified as a robust high-lipid- producing Scenedesmus spp. a) When cultured at neutral pH the cells proliferate and maintain a green pigmentation. When cultured under low pH conditions (pH = 3), the microalgae take on a yellow/gold pigmentation. b) Gravimetric lipid analysis demonstrates increased in lipid content when cultured at pH = 3 over the course of 7–28 days.

Mentions: When cultured in low pH conditions (pH = 3) for 14 days, Lig 290 underwent a physiological shift changing in color from green (chlorophyll rich) to gold (lipid rich) color as demonstrated in the panel insert of Figure 4a. We also observed a change in morphology where Scenedesmus spp. quartet coenobia disassociated into a unicellular form that we ascribe to a stressed state.


Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Lig 290 is identified as a robust high-lipid- producing Scenedesmus spp. a) When cultured at neutral pH the cells proliferate and maintain a green pigmentation. When cultured under low pH conditions (pH = 3), the microalgae take on a yellow/gold pigmentation. b) Gravimetric lipid analysis demonstrates increased in lipid content when cultured at pH = 3 over the course of 7–28 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230719&req=5

Figure 4: Lig 290 is identified as a robust high-lipid- producing Scenedesmus spp. a) When cultured at neutral pH the cells proliferate and maintain a green pigmentation. When cultured under low pH conditions (pH = 3), the microalgae take on a yellow/gold pigmentation. b) Gravimetric lipid analysis demonstrates increased in lipid content when cultured at pH = 3 over the course of 7–28 days.
Mentions: When cultured in low pH conditions (pH = 3) for 14 days, Lig 290 underwent a physiological shift changing in color from green (chlorophyll rich) to gold (lipid rich) color as demonstrated in the panel insert of Figure 4a. We also observed a change in morphology where Scenedesmus spp. quartet coenobia disassociated into a unicellular form that we ascribe to a stressed state.

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus