Limits...
Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus

Visual assessment of Lig 290. An environmental sample direct from location Lig 290 and an axenic culture of Lig 290 were visualized via bright field microscopy. a) The environmental sample contained a communities of unicellular, multicellular and filamentous green microalgae. b) An isolated Scendesmus spp. was isolated from the environmental sample. Images are 40× and 100× objective magnification. White scale bars provided at the top left are 100 μm.
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Figure 3: Visual assessment of Lig 290. An environmental sample direct from location Lig 290 and an axenic culture of Lig 290 were visualized via bright field microscopy. a) The environmental sample contained a communities of unicellular, multicellular and filamentous green microalgae. b) An isolated Scendesmus spp. was isolated from the environmental sample. Images are 40× and 100× objective magnification. White scale bars provided at the top left are 100 μm.

Mentions: Microscopic assessment of Lig 290 indicated a community of microorganisms including filamentous and unicellular green microalgae (Figure 3a). Of the photosynthetic organisms, morphological analysis indicated that Scenedesmus was the most prevalent genus of green microalgae. An axenic culture was obtained using a standard agar streaking technique. Figure 3b illustrates the resulting Lig 290 axenic culture.


Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Visual assessment of Lig 290. An environmental sample direct from location Lig 290 and an axenic culture of Lig 290 were visualized via bright field microscopy. a) The environmental sample contained a communities of unicellular, multicellular and filamentous green microalgae. b) An isolated Scendesmus spp. was isolated from the environmental sample. Images are 40× and 100× objective magnification. White scale bars provided at the top left are 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230719&req=5

Figure 3: Visual assessment of Lig 290. An environmental sample direct from location Lig 290 and an axenic culture of Lig 290 were visualized via bright field microscopy. a) The environmental sample contained a communities of unicellular, multicellular and filamentous green microalgae. b) An isolated Scendesmus spp. was isolated from the environmental sample. Images are 40× and 100× objective magnification. White scale bars provided at the top left are 100 μm.
Mentions: Microscopic assessment of Lig 290 indicated a community of microorganisms including filamentous and unicellular green microalgae (Figure 3a). Of the photosynthetic organisms, morphological analysis indicated that Scenedesmus was the most prevalent genus of green microalgae. An axenic culture was obtained using a standard agar streaking technique. Figure 3b illustrates the resulting Lig 290 axenic culture.

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus