Limits...
Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus

Screening of environmental samples for high-lipid producing microalgae. Analysis of Nile Red stained samples via flow cytometry identified Lig 290 as a high lipid producing sample. Experimental results are based on a mean analysis of 10,000 events and error bars represent standard error of the mean.
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Figure 2: Screening of environmental samples for high-lipid producing microalgae. Analysis of Nile Red stained samples via flow cytometry identified Lig 290 as a high lipid producing sample. Experimental results are based on a mean analysis of 10,000 events and error bars represent standard error of the mean.

Mentions: To identify algal species that could be high-lipid producing algae, we employed a lipophilic Nile Red screening strategy as a means of quantifying relative lipid levels. Microalgae collected from the wild were stained and analyzed via flow cytometry (Cirulis et al.2012). Figure 2 illustrates Nile Red screening of environmental samples collected in proximity to the Onakawana mine. Interestingly, the two samples having the highest Nile Red signal (Lig 266 and Lig 290) were isolated from a low pH environment (pH <5), and both of these samples produced higher signals than a laboratory strain Scenedesmus dimorphus) commonly used in biofuel studies. Due to Lig 290 producing the highest lipophilic signal and being identified from a low pH environment, we chose to isolate the strains of microalgae in the hope of identifying a high lipid variety. Thus, we selected Lig 290 as a candidate sample to characterize as a potential high-lipid producing pH tolerant microalgae strain, which could be used in biofuel production.


Bioprospecting for acidophilic lipid-rich green microalgae isolated from abandoned mine site water bodies.

Eibl JK, Corcoran JD, Senhorinho GN, Zhang K, Hosseini NS, Marsden J, Laamanen CA, Scott JA, Ross GM - AMB Express (2014)

Screening of environmental samples for high-lipid producing microalgae. Analysis of Nile Red stained samples via flow cytometry identified Lig 290 as a high lipid producing sample. Experimental results are based on a mean analysis of 10,000 events and error bars represent standard error of the mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230719&req=5

Figure 2: Screening of environmental samples for high-lipid producing microalgae. Analysis of Nile Red stained samples via flow cytometry identified Lig 290 as a high lipid producing sample. Experimental results are based on a mean analysis of 10,000 events and error bars represent standard error of the mean.
Mentions: To identify algal species that could be high-lipid producing algae, we employed a lipophilic Nile Red screening strategy as a means of quantifying relative lipid levels. Microalgae collected from the wild were stained and analyzed via flow cytometry (Cirulis et al.2012). Figure 2 illustrates Nile Red screening of environmental samples collected in proximity to the Onakawana mine. Interestingly, the two samples having the highest Nile Red signal (Lig 266 and Lig 290) were isolated from a low pH environment (pH <5), and both of these samples produced higher signals than a laboratory strain Scenedesmus dimorphus) commonly used in biofuel studies. Due to Lig 290 producing the highest lipophilic signal and being identified from a low pH environment, we chose to isolate the strains of microalgae in the hope of identifying a high lipid variety. Thus, we selected Lig 290 as a candidate sample to characterize as a potential high-lipid producing pH tolerant microalgae strain, which could be used in biofuel production.

Bottom Line: Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains.Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada.As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Northern Ontario School of Medicine, 935 Ramsey Lake Rd, Sudbury, ON P3E 2C6, Canada. gross@nosm.ca.

ABSTRACT
With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae species are found to be sensitive to environmental stresses or competition by regional strains. Contamination by invasive species can diminish productivity of commercial algal processes. A potential improvement to current strategies is to identify high-lipid producing microalgae, which thrive in selected culture conditions that reduce the risk of contamination, such as low pH. Here we report the identification of a novel high-lipid producing microalgae which can tolerate low pH growth conditions. Lig 290 is a Scenedesmus spp. isolated from a low pH waterbody (pH = 4.5) in proximity to an abandoned lignite mine in Northern Ontario, Canada. Compared to a laboratory strain of Scendesmus dimorphus, Lig 290 demonstrated robust growth rates, a strong growth profile, and high lipid production. As a consequence, Lig 290 may have potential application as a robust microalgal species for use in biofuel production.

No MeSH data available.


Related in: MedlinePlus