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Phototherapy promotes healing of cutaneous wounds in undernourished rats.

Leite SN, Andrade TA, Masson-Meyers Ddos S, Leite MN, Enwemeka CS, Frade MA - An Bras Dermatol (2014 Nov-Dec)

Bottom Line: Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.Similar beneficial effects may be achieved in patients with poor nutritional status.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

ABSTRACT

Background: Various studies have shown that phototherapy promotes the healing of cutaneous wounds.

Objective: To investigate the effect of phototherapy on healing of cutaneous wounds in nourished and undernourished rats.

Methods: Forty rats, 20 nourished plus 20 others rendered marasmus with undernourishment, were assigned to four equal groups: nourished sham, nourished Light Emitting Diode treated, undernourished sham and undernourished Light Emitting Diode treated. In the two treated groups, two 8-mm punch wounds made on the dorsum of each rat were irradiated three times per week with 3 J/cm2 sq cm of combined 660 and 890 nm light; wounds in the other groups were not irradiated. Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.

Results: Undernourishment retarded the mean healing rate of the undernourished sham wounds (p < 0.01), but not the undernourished Light emission diode treated wounds, which healed significantly faster (p < 0.001) and as fast as the two nourished groups. Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.

Conclusion: Phototherapy reverses the adverse healing effects of undernourishment. Similar beneficial effects may be achieved in patients with poor nutritional status.

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Related in: MedlinePlus

Percentage of collagen area quantified in each group of wounds on the14th postoperative day
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f04: Percentage of collagen area quantified in each group of wounds on the14th postoperative day

Mentions: As shown in graph 3 and figure 2, the US wounds also had a smaller percentage of collagenthan those of any other group (p <0.01). Qualitative histological analysis showedthat the healed tissue had a lighter blue color-different from the normal collagencolor seen in the other groups. The US group also had smaller, less dense collagenformation compared with the other groups (Figure2). In contrast, the collagen of undernourished wounds, treated with LEDphototherapy, was visibly superior compared with the US group and similar inappearance to the specimens from the two nourished groups (Figure 2).


Phototherapy promotes healing of cutaneous wounds in undernourished rats.

Leite SN, Andrade TA, Masson-Meyers Ddos S, Leite MN, Enwemeka CS, Frade MA - An Bras Dermatol (2014 Nov-Dec)

Percentage of collagen area quantified in each group of wounds on the14th postoperative day
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230658&req=5

f04: Percentage of collagen area quantified in each group of wounds on the14th postoperative day
Mentions: As shown in graph 3 and figure 2, the US wounds also had a smaller percentage of collagenthan those of any other group (p <0.01). Qualitative histological analysis showedthat the healed tissue had a lighter blue color-different from the normal collagencolor seen in the other groups. The US group also had smaller, less dense collagenformation compared with the other groups (Figure2). In contrast, the collagen of undernourished wounds, treated with LEDphototherapy, was visibly superior compared with the US group and similar inappearance to the specimens from the two nourished groups (Figure 2).

Bottom Line: Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.Similar beneficial effects may be achieved in patients with poor nutritional status.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

ABSTRACT

Background: Various studies have shown that phototherapy promotes the healing of cutaneous wounds.

Objective: To investigate the effect of phototherapy on healing of cutaneous wounds in nourished and undernourished rats.

Methods: Forty rats, 20 nourished plus 20 others rendered marasmus with undernourishment, were assigned to four equal groups: nourished sham, nourished Light Emitting Diode treated, undernourished sham and undernourished Light Emitting Diode treated. In the two treated groups, two 8-mm punch wounds made on the dorsum of each rat were irradiated three times per week with 3 J/cm2 sq cm of combined 660 and 890 nm light; wounds in the other groups were not irradiated. Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.

Results: Undernourishment retarded the mean healing rate of the undernourished sham wounds (p < 0.01), but not the undernourished Light emission diode treated wounds, which healed significantly faster (p < 0.001) and as fast as the two nourished groups. Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.

Conclusion: Phototherapy reverses the adverse healing effects of undernourishment. Similar beneficial effects may be achieved in patients with poor nutritional status.

Show MeSH
Related in: MedlinePlus