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Phototherapy promotes healing of cutaneous wounds in undernourished rats.

Leite SN, Andrade TA, Masson-Meyers Ddos S, Leite MN, Enwemeka CS, Frade MA - An Bras Dermatol (2014 Nov-Dec)

Bottom Line: Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.Similar beneficial effects may be achieved in patients with poor nutritional status.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

ABSTRACT

Background: Various studies have shown that phototherapy promotes the healing of cutaneous wounds.

Objective: To investigate the effect of phototherapy on healing of cutaneous wounds in nourished and undernourished rats.

Methods: Forty rats, 20 nourished plus 20 others rendered marasmus with undernourishment, were assigned to four equal groups: nourished sham, nourished Light Emitting Diode treated, undernourished sham and undernourished Light Emitting Diode treated. In the two treated groups, two 8-mm punch wounds made on the dorsum of each rat were irradiated three times per week with 3 J/cm2 sq cm of combined 660 and 890 nm light; wounds in the other groups were not irradiated. Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.

Results: Undernourishment retarded the mean healing rate of the undernourished sham wounds (p < 0.01), but not the undernourished Light emission diode treated wounds, which healed significantly faster (p < 0.001) and as fast as the two nourished groups. Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.

Conclusion: Phototherapy reverses the adverse healing effects of undernourishment. Similar beneficial effects may be achieved in patients with poor nutritional status.

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Related in: MedlinePlus

Mean ulcers healing rate (UHR) evaluated on the 7th postoperative day(A) and the 14th postoperative day (B)
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f02: Mean ulcers healing rate (UHR) evaluated on the 7th postoperative day(A) and the 14th postoperative day (B)

Mentions: Day seven data showed that undernourished sham (US) wounds healed significantlyslower than any of the other wounds, and was statistically different from the NSgroup (p<0.01), NL group (p<0.05) and the UL group (p<0.001), as displayedin graph 2A. Treatment with LED phototherapyreversed this trend, such that UL wounds healed significantly faster than the USwounds (p<0.001) and attained the same healing rate as treated or untreatednourished wounds (Graph 2A). Thus, phototherapyfully reversed the adverse healing effect of marasmus, restoring normal healing inthe UL group. There was no statistically significant difference in the healing ratesof nourished and undernourished wounds treated with phototherapy; neither were theredifferences in UHR between the UL and NL groups.


Phototherapy promotes healing of cutaneous wounds in undernourished rats.

Leite SN, Andrade TA, Masson-Meyers Ddos S, Leite MN, Enwemeka CS, Frade MA - An Bras Dermatol (2014 Nov-Dec)

Mean ulcers healing rate (UHR) evaluated on the 7th postoperative day(A) and the 14th postoperative day (B)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4230658&req=5

f02: Mean ulcers healing rate (UHR) evaluated on the 7th postoperative day(A) and the 14th postoperative day (B)
Mentions: Day seven data showed that undernourished sham (US) wounds healed significantlyslower than any of the other wounds, and was statistically different from the NSgroup (p<0.01), NL group (p<0.05) and the UL group (p<0.001), as displayedin graph 2A. Treatment with LED phototherapyreversed this trend, such that UL wounds healed significantly faster than the USwounds (p<0.001) and attained the same healing rate as treated or untreatednourished wounds (Graph 2A). Thus, phototherapyfully reversed the adverse healing effect of marasmus, restoring normal healing inthe UL group. There was no statistically significant difference in the healing ratesof nourished and undernourished wounds treated with phototherapy; neither were theredifferences in UHR between the UL and NL groups.

Bottom Line: Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.Similar beneficial effects may be achieved in patients with poor nutritional status.

View Article: PubMed Central - PubMed

Affiliation: Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

ABSTRACT

Background: Various studies have shown that phototherapy promotes the healing of cutaneous wounds.

Objective: To investigate the effect of phototherapy on healing of cutaneous wounds in nourished and undernourished rats.

Methods: Forty rats, 20 nourished plus 20 others rendered marasmus with undernourishment, were assigned to four equal groups: nourished sham, nourished Light Emitting Diode treated, undernourished sham and undernourished Light Emitting Diode treated. In the two treated groups, two 8-mm punch wounds made on the dorsum of each rat were irradiated three times per week with 3 J/cm2 sq cm of combined 660 and 890 nm light; wounds in the other groups were not irradiated. Wounds were evaluated with digital photography and image analysis, either on day 7 or day 14, with biopsies obtained on day 14 for histological studies.

Results: Undernourishment retarded the mean healing rate of the undernourished sham wounds (p < 0.01), but not the undernourished Light emission diode treated wounds, which healed significantly faster (p < 0.001) and as fast as the two nourished groups. Histological analysis showed a smaller percentage of collagen in the undernourished sham group compared with the three other groups, thus confirming our photographic image analysis data.

Conclusion: Phototherapy reverses the adverse healing effects of undernourishment. Similar beneficial effects may be achieved in patients with poor nutritional status.

Show MeSH
Related in: MedlinePlus