World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. Fluid biospecimen collection, processing, and storage in endometriosis research.
Bottom Line: The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies.The SOPs will be reviewed based on investigator feedback, and through systematic tri-annual follow-up.Updated versions will be made available at: endometriosisfoundation.org/ephect.
Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.Show MeSH
Related in: MedlinePlus
Mentions: Blood is most usefully banked after separation into its derivatives (serum, plasma, and red/white blood cells), to allow the widest possible future use (Fig. 2). Peripheral blood allows the measurement of a broad range of biomolecules, in both patients and healthy volunteers, and relatively large volumes can be collected. However, peripheral blood includes a complex mix of molecules reflecting many biologic processes in the body, in which biologic changes relevant to the disease may not be detected as readily as in a disease-related tissue. When collecting blood samples for a study, several important decisions need to be made regarding:  timing and conditions of sample collection;  use of anticoagulants and clot accelerators in collection tubes;  sample stability between collection and processing;  processing; and  long-term storage. We describe important aspects of each of these, and how they are dealt with in the SOPs. Eighteen EPHect centers provided blood SOPs for consensus agreement.
Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.