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World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. Fluid biospecimen collection, processing, and storage in endometriosis research.

Rahmioglu N, Fassbender A, Vitonis AF, Tworoger SS, Hummelshoj L, D'Hooghe TM, Adamson GD, Giudice LC, Becker CM, Zondervan KT, Missmer SA, WERF EPHect Working Gro - Fertil. Steril. (2014)

Bottom Line: The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies.The SOPs will be reviewed based on investigator feedback, and through systematic tri-annual follow-up.Updated versions will be made available at: endometriosisfoundation.org/ephect.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.

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Related in: MedlinePlus

Potential uses of blood constituents in genetic, expression, protein, and metabolite analyses.
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Related In: Results  -  Collection


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fig2: Potential uses of blood constituents in genetic, expression, protein, and metabolite analyses.

Mentions: Blood is most usefully banked after separation into its derivatives (serum, plasma, and red/white blood cells), to allow the widest possible future use (Fig. 2). Peripheral blood allows the measurement of a broad range of biomolecules, in both patients and healthy volunteers, and relatively large volumes can be collected. However, peripheral blood includes a complex mix of molecules reflecting many biologic processes in the body, in which biologic changes relevant to the disease may not be detected as readily as in a disease-related tissue. When collecting blood samples for a study, several important decisions need to be made regarding: [1] timing and conditions of sample collection; [2] use of anticoagulants and clot accelerators in collection tubes; [3] sample stability between collection and processing; [4] processing; and [5] long-term storage. We describe important aspects of each of these, and how they are dealt with in the SOPs. Eighteen EPHect centers provided blood SOPs for consensus agreement.


World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. Fluid biospecimen collection, processing, and storage in endometriosis research.

Rahmioglu N, Fassbender A, Vitonis AF, Tworoger SS, Hummelshoj L, D'Hooghe TM, Adamson GD, Giudice LC, Becker CM, Zondervan KT, Missmer SA, WERF EPHect Working Gro - Fertil. Steril. (2014)

Potential uses of blood constituents in genetic, expression, protein, and metabolite analyses.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230639&req=5

fig2: Potential uses of blood constituents in genetic, expression, protein, and metabolite analyses.
Mentions: Blood is most usefully banked after separation into its derivatives (serum, plasma, and red/white blood cells), to allow the widest possible future use (Fig. 2). Peripheral blood allows the measurement of a broad range of biomolecules, in both patients and healthy volunteers, and relatively large volumes can be collected. However, peripheral blood includes a complex mix of molecules reflecting many biologic processes in the body, in which biologic changes relevant to the disease may not be detected as readily as in a disease-related tissue. When collecting blood samples for a study, several important decisions need to be made regarding: [1] timing and conditions of sample collection; [2] use of anticoagulants and clot accelerators in collection tubes; [3] sample stability between collection and processing; [4] processing; and [5] long-term storage. We describe important aspects of each of these, and how they are dealt with in the SOPs. Eighteen EPHect centers provided blood SOPs for consensus agreement.

Bottom Line: The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies.The SOPs will be reviewed based on investigator feedback, and through systematic tri-annual follow-up.Updated versions will be made available at: endometriosisfoundation.org/ephect.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.

Show MeSH
Related in: MedlinePlus