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World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. Fluid biospecimen collection, processing, and storage in endometriosis research.

Rahmioglu N, Fassbender A, Vitonis AF, Tworoger SS, Hummelshoj L, D'Hooghe TM, Adamson GD, Giudice LC, Becker CM, Zondervan KT, Missmer SA, WERF EPHect Working Gro - Fertil. Steril. (2014)

Bottom Line: The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies.The SOPs will be reviewed based on investigator feedback, and through systematic tri-annual follow-up.Updated versions will be made available at: endometriosisfoundation.org/ephect.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.

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Related in: MedlinePlus

Flow diagram depicting the WERF EPHect development and consensus process (biological fluid sample SOPs).
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Related In: Results  -  Collection


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fig1: Flow diagram depicting the WERF EPHect development and consensus process (biological fluid sample SOPs).

Mentions: We conducted 2 workshops in March and July 2013, followed by several rounds of expert review, bringing together 54 leaders in endometriosis research and management and in sample processing from 34 clinical/academic centers and 3 industry collaborators in 16 countries to develop and reach consensus on evidence-based phenome collection and SOP guidelines (Fig. 1; 20). During workshop I and a subsequent consultation round, we identified 18 centers worldwide that collect biologic fluid samples from endometriosis cases and controls on a large scale (criterion: publication on >100 cases); all provided SOPs for sample collection, processing, and storage. Six fluid sample types were collected by the centers (blood, urine, saliva, peritoneal fluid, endometrial fluid, and menstrual fluid; Table 2). Quantities collected were often a balance between volumes sufficient to conduct a wide range of future experiments (biobanking); participant-based acceptability; and the costs of collection, processing, and long-term storage.


World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. Fluid biospecimen collection, processing, and storage in endometriosis research.

Rahmioglu N, Fassbender A, Vitonis AF, Tworoger SS, Hummelshoj L, D'Hooghe TM, Adamson GD, Giudice LC, Becker CM, Zondervan KT, Missmer SA, WERF EPHect Working Gro - Fertil. Steril. (2014)

Flow diagram depicting the WERF EPHect development and consensus process (biological fluid sample SOPs).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230639&req=5

fig1: Flow diagram depicting the WERF EPHect development and consensus process (biological fluid sample SOPs).
Mentions: We conducted 2 workshops in March and July 2013, followed by several rounds of expert review, bringing together 54 leaders in endometriosis research and management and in sample processing from 34 clinical/academic centers and 3 industry collaborators in 16 countries to develop and reach consensus on evidence-based phenome collection and SOP guidelines (Fig. 1; 20). During workshop I and a subsequent consultation round, we identified 18 centers worldwide that collect biologic fluid samples from endometriosis cases and controls on a large scale (criterion: publication on >100 cases); all provided SOPs for sample collection, processing, and storage. Six fluid sample types were collected by the centers (blood, urine, saliva, peritoneal fluid, endometrial fluid, and menstrual fluid; Table 2). Quantities collected were often a balance between volumes sufficient to conduct a wide range of future experiments (biobanking); participant-based acceptability; and the costs of collection, processing, and long-term storage.

Bottom Line: The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies.The SOPs will be reviewed based on investigator feedback, and through systematic tri-annual follow-up.Updated versions will be made available at: endometriosisfoundation.org/ephect.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.

Show MeSH
Related in: MedlinePlus