World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonization Project: III. Fluid biospecimen collection, processing, and storage in endometriosis research.
Bottom Line: The consensus SOPs are based on the best available evidence; areas with limited evidence are identified as requiring further pilot studies.The SOPs will be reviewed based on investigator feedback, and through systematic tri-annual follow-up.Updated versions will be made available at: endometriosisfoundation.org/ephect.
Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.Show MeSH
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Mentions: We conducted 2 workshops in March and July 2013, followed by several rounds of expert review, bringing together 54 leaders in endometriosis research and management and in sample processing from 34 clinical/academic centers and 3 industry collaborators in 16 countries to develop and reach consensus on evidence-based phenome collection and SOP guidelines (Fig. 1; 20). During workshop I and a subsequent consultation round, we identified 18 centers worldwide that collect biologic fluid samples from endometriosis cases and controls on a large scale (criterion: publication on >100 cases); all provided SOPs for sample collection, processing, and storage. Six fluid sample types were collected by the centers (blood, urine, saliva, peritoneal fluid, endometrial fluid, and menstrual fluid; Table 2). Quantities collected were often a balance between volumes sufficient to conduct a wide range of future experiments (biobanking); participant-based acceptability; and the costs of collection, processing, and long-term storage.
Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.