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Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport.

Chiba K, Araseki M, Nozawa K, Furukori K, Araki Y, Matsushima T, Nakaya T, Hata S, Saito Y, Uchida S, Okada Y, Nairn AC, Davis RJ, Yamamoto T, Kinjo M, Taru H, Suzuki T - Mol. Biol. Cell (2014)

Bottom Line: In JIP1-deficient neurons, we find that both the fast velocity (∼2.7 μm/s) and high frequency (66%) of anterograde transport of APP cargo are impaired to a reduced velocity (∼1.83 μm/s) and a lower frequency (45%).Furthermore, efficient APP axonal transport is not influenced by phosphorylation of APP at Thr-668, a site known to be phosphorylated by JNK.Our quantitative analysis indicates that enhanced fast-velocity and efficient high-frequency APP anterograde transport observed in neurons are mediated by novel roles of JIP1b.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroscience, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.

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Role of novel KLC-binding regions of JIP1b in efficient anterograde transport of APP cargo. APP-EGFP and the indicated JIP1b mutants were expressed in primary cultured neurons from JIP1-KO mice (A–D). Data are presented as in Figure 1. The transport of APP cargo is shown. Position of average velocity is indicated with arrows. See Supplemental Movie S3A for A, S3B for B, S3C for C, and S3D for D. Scale bar, 5 μm. The ratios of anterograde (A), retrograde (R), and stationary (S) vesicles are indicated in the insets at the right. Statistical analysis for direction of movement vesicles is summarized in Table 1.
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Figure 5: Role of novel KLC-binding regions of JIP1b in efficient anterograde transport of APP cargo. APP-EGFP and the indicated JIP1b mutants were expressed in primary cultured neurons from JIP1-KO mice (A–D). Data are presented as in Figure 1. The transport of APP cargo is shown. Position of average velocity is indicated with arrows. See Supplemental Movie S3A for A, S3B for B, S3C for C, and S3D for D. Scale bar, 5 μm. The ratios of anterograde (A), retrograde (R), and stationary (S) vesicles are indicated in the insets at the right. Statistical analysis for direction of movement vesicles is summarized in Table 1.

Mentions: Results of Figures 1, 2, A and B, and 5 are summarized, with statistical analysis for the ratio of movement directions. APP movement in JIP1−/− neurons is compared with that in JIP1+/+ neurons in the first row, and APP movement in JIP1−/− neurons expressing JIP1b proteins is compared with that in JIP1−/− neurons in subsequent rows.


Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport.

Chiba K, Araseki M, Nozawa K, Furukori K, Araki Y, Matsushima T, Nakaya T, Hata S, Saito Y, Uchida S, Okada Y, Nairn AC, Davis RJ, Yamamoto T, Kinjo M, Taru H, Suzuki T - Mol. Biol. Cell (2014)

Role of novel KLC-binding regions of JIP1b in efficient anterograde transport of APP cargo. APP-EGFP and the indicated JIP1b mutants were expressed in primary cultured neurons from JIP1-KO mice (A–D). Data are presented as in Figure 1. The transport of APP cargo is shown. Position of average velocity is indicated with arrows. See Supplemental Movie S3A for A, S3B for B, S3C for C, and S3D for D. Scale bar, 5 μm. The ratios of anterograde (A), retrograde (R), and stationary (S) vesicles are indicated in the insets at the right. Statistical analysis for direction of movement vesicles is summarized in Table 1.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230617&req=5

Figure 5: Role of novel KLC-binding regions of JIP1b in efficient anterograde transport of APP cargo. APP-EGFP and the indicated JIP1b mutants were expressed in primary cultured neurons from JIP1-KO mice (A–D). Data are presented as in Figure 1. The transport of APP cargo is shown. Position of average velocity is indicated with arrows. See Supplemental Movie S3A for A, S3B for B, S3C for C, and S3D for D. Scale bar, 5 μm. The ratios of anterograde (A), retrograde (R), and stationary (S) vesicles are indicated in the insets at the right. Statistical analysis for direction of movement vesicles is summarized in Table 1.
Mentions: Results of Figures 1, 2, A and B, and 5 are summarized, with statistical analysis for the ratio of movement directions. APP movement in JIP1−/− neurons is compared with that in JIP1+/+ neurons in the first row, and APP movement in JIP1−/− neurons expressing JIP1b proteins is compared with that in JIP1−/− neurons in subsequent rows.

Bottom Line: In JIP1-deficient neurons, we find that both the fast velocity (∼2.7 μm/s) and high frequency (66%) of anterograde transport of APP cargo are impaired to a reduced velocity (∼1.83 μm/s) and a lower frequency (45%).Furthermore, efficient APP axonal transport is not influenced by phosphorylation of APP at Thr-668, a site known to be phosphorylated by JNK.Our quantitative analysis indicates that enhanced fast-velocity and efficient high-frequency APP anterograde transport observed in neurons are mediated by novel roles of JIP1b.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroscience, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.

Show MeSH
Related in: MedlinePlus