Quantitative analysis of APP axonal transport in neurons: role of JIP1 in enhanced APP anterograde transport.
Bottom Line: In JIP1-deficient neurons, we find that both the fast velocity (∼2.7 μm/s) and high frequency (66%) of anterograde transport of APP cargo are impaired to a reduced velocity (∼1.83 μm/s) and a lower frequency (45%).Furthermore, efficient APP axonal transport is not influenced by phosphorylation of APP at Thr-668, a site known to be phosphorylated by JNK.Our quantitative analysis indicates that enhanced fast-velocity and efficient high-frequency APP anterograde transport observed in neurons are mediated by novel roles of JIP1b.
Affiliation: Laboratory of Neuroscience, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.Show MeSH
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Mentions: Results of Figures 1, 2, A and B, and 5 are summarized, with statistical analysis for the ratio of movement directions. APP movement in JIP1−/− neurons is compared with that in JIP1+/+ neurons in the first row, and APP movement in JIP1−/− neurons expressing JIP1b proteins is compared with that in JIP1−/− neurons in subsequent rows.
Affiliation: Laboratory of Neuroscience, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.