Single-molecule tracking of tau reveals fast kiss-and-hop interaction with microtubules in living neurons.
Bottom Line: Furthermore, we observed by quantitative imaging using fluorescence decay after photoactivation recordings of photoactivatable GFP-tagged tubulin that, despite this rapid dynamics, tau is capable of regulating the tubulin-microtubule balance.Our data imply a novel kiss-and-hop mechanism by which tau promotes neuronal microtubule assembly.The rapid kiss-and-hop interaction explains why tau, although binding to microtubules, does not interfere with axonal transport.
Affiliation: Department of Neurobiology, University of Osnabrück, D-49076 Osnabrück, Germany.Show MeSH
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Mentions: PC12 cells are a well-characterized neuronal model with a rather homogeneous cell population and well-defined microtubule distribution in processes. They do not, however, develop axonal-somatodendritic polarity. To examine the behavior of tau in axons, we prepared primary mouse cortical cultures and introduced Halo-tagged tau by lentiviral gene transfer (Bakota et al., 2012; Figure 6A, left). To avoid potential interference of endogenous mouse tau, we prepared cultures from TAU−/− animals. A Western blot confirmed 1) the absence of endogenous mouse tau in the TAU−/− cultures (arrowheads, Figure 6A, right) and 2) the expression of Halo-tau at a level comparable to that of the endogenous one (arrow, Figure 6A, right).
Affiliation: Department of Neurobiology, University of Osnabrück, D-49076 Osnabrück, Germany.