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Single-molecule tracking of tau reveals fast kiss-and-hop interaction with microtubules in living neurons.

Janning D, Igaev M, Sündermann F, Brühmann J, Beutel O, Heinisch JJ, Bakota L, Piehler J, Junge W, Brandt R - Mol. Biol. Cell (2014)

Bottom Line: Furthermore, we observed by quantitative imaging using fluorescence decay after photoactivation recordings of photoactivatable GFP-tagged tubulin that, despite this rapid dynamics, tau is capable of regulating the tubulin-microtubule balance.Our data imply a novel kiss-and-hop mechanism by which tau promotes neuronal microtubule assembly.The rapid kiss-and-hop interaction explains why tau, although binding to microtubules, does not interfere with axonal transport.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurobiology, University of Osnabrück, D-49076 Osnabrück, Germany.

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Related in: MedlinePlus

Representative histogram of residence times of tau on microtubules in PC12 cells. The monoexponential fit is shown by a red line, and the time constant (dwell time) is indicated on top.
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Figure 4: Representative histogram of residence times of tau on microtubules in PC12 cells. The monoexponential fit is shown by a red line, and the time constant (dwell time) is indicated on top.

Mentions: Single-molecule experiments allow estimating the dwell time of tau on a single microtubule. The respective frequency histograms of residence times of tau on MTs could be best fitted to a single exponential, indicating that tau has only one major interaction partner in neuritic shafts, namely, MTs. Time constants of the exponential fits were referred to as dwell times (Figure 4). We found that tau dwells on a single MT for ∼40 ms, which is shorter by two orders of magnitude than previously reported (Konzack et al., 2007).


Single-molecule tracking of tau reveals fast kiss-and-hop interaction with microtubules in living neurons.

Janning D, Igaev M, Sündermann F, Brühmann J, Beutel O, Heinisch JJ, Bakota L, Piehler J, Junge W, Brandt R - Mol. Biol. Cell (2014)

Representative histogram of residence times of tau on microtubules in PC12 cells. The monoexponential fit is shown by a red line, and the time constant (dwell time) is indicated on top.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230615&req=5

Figure 4: Representative histogram of residence times of tau on microtubules in PC12 cells. The monoexponential fit is shown by a red line, and the time constant (dwell time) is indicated on top.
Mentions: Single-molecule experiments allow estimating the dwell time of tau on a single microtubule. The respective frequency histograms of residence times of tau on MTs could be best fitted to a single exponential, indicating that tau has only one major interaction partner in neuritic shafts, namely, MTs. Time constants of the exponential fits were referred to as dwell times (Figure 4). We found that tau dwells on a single MT for ∼40 ms, which is shorter by two orders of magnitude than previously reported (Konzack et al., 2007).

Bottom Line: Furthermore, we observed by quantitative imaging using fluorescence decay after photoactivation recordings of photoactivatable GFP-tagged tubulin that, despite this rapid dynamics, tau is capable of regulating the tubulin-microtubule balance.Our data imply a novel kiss-and-hop mechanism by which tau promotes neuronal microtubule assembly.The rapid kiss-and-hop interaction explains why tau, although binding to microtubules, does not interfere with axonal transport.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurobiology, University of Osnabrück, D-49076 Osnabrück, Germany.

Show MeSH
Related in: MedlinePlus