Single-molecule tracking of tau reveals fast kiss-and-hop interaction with microtubules in living neurons.
Bottom Line: Furthermore, we observed by quantitative imaging using fluorescence decay after photoactivation recordings of photoactivatable GFP-tagged tubulin that, despite this rapid dynamics, tau is capable of regulating the tubulin-microtubule balance.Our data imply a novel kiss-and-hop mechanism by which tau promotes neuronal microtubule assembly.The rapid kiss-and-hop interaction explains why tau, although binding to microtubules, does not interfere with axonal transport.
Affiliation: Department of Neurobiology, University of Osnabrück, D-49076 Osnabrück, Germany.Show MeSH
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Mentions: To assess the behavior of tau in neuronal processes, single, fluorescently labeled tau molecules were tracked with a temporal resolution of 5 ms. HaloTag was fused to the amino terminus of human tau, where it does not interfere with the MT interaction that is mediated by tau's carboxy-terminal half (Gauthier-Kemper et al., 2011; Figure 1A, top). The focal plane of the total internal reflection fluorescence (TIRF) microscope was adjusted to capture MTs (Figure 1A).
Affiliation: Department of Neurobiology, University of Osnabrück, D-49076 Osnabrück, Germany.