TGF-β regulates LARG and GEF-H1 during EMT to affect stiffening response to force and cell invasion.
Bottom Line: Recent studies implicate a role for cell mechanics in cancer progression.Previously, force application on integrins has been shown to initiate cytoskeletal rearrangements that result in increased cell stiffness and a stiffening response.Here we demonstrate that transforming growth factor β (TGF-β)-induced EMT results in decreased stiffness and loss of the normal stiffening response to force applied on integrins.
Affiliation: Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.Show MeSH
Related in: MedlinePlus
Mentions: To determine whether the decrease in stiffness observed after EMT was a result of the force magnitude applied during the magnetic tweezers (active microrheology) assay, we performed an external passive microrheology assay to measure the basal stiffness of NMuMG cells before and after TGF-β–initiated EMT. Consistent with observations with the magnetic tweezers assay, measurement of the thermal motion of FN-coated beads revealed a threefold decrease in cell stiffness upon TGF-β treatment (Figure 3, A and B).
Affiliation: Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.