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Synergies between Aip1p and capping protein subunits (Acp1p and Acp2p) in clathrin-mediated endocytosis and cell polarization in fission yeast.

Berro J, Pollard TD - Mol. Biol. Cell (2014)

Bottom Line: Capping protein and Aip1p help maintain the high density of actin filaments in meshwork by keeping actin filaments close enough for cross-linking.Our experiments also reveal new cellular functions for Acp1p and Acp2p independent of their capping activity.We identified two independent pathways that control polarization of endocytic sites, one depending on acp2(+) and aip1(+) during interphase and the other independent of acp1(+), acp2(+), and aip1(+) during mitosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103 Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8103 Nanobiology Institute, Yale University, New Haven, CT 06520-8103 Institut Camille Jordan, UMR CNRS 5208, Université de Lyon, 69622 Villeurbanne-Cedex, France Centre de Génétique et de Physiologie Moléculaire et Cellulaire, UMR CNRS 5534, Université de Lyon, 69622 Villeurbanne-Cedex, France.

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Endocytic patch polarization and dispersion in wild-type and mutant cells. (A) Negative-contrast fluorescence micrographs of representative cells expressing Fim1p-mEGFP in seven strains indicated at the top of each set. First row, short interphase cells; middle row, medium-sized interphase cells; third row, cells in cytokinesis. (B) Graphs of the total number of patches per cell marked with Fim1p-mEGFP vs. the cell length for the strain indicated above each panel. Each symbol corresponds to an individual cell in interphase (●) or cytokinesis (+). Lines are the best linear correlations for the data (blue, y-intercept equals 0; black, free y-intercept). The equations for these lines are given at the bottom right of each graph. (C) Distributions of endocytic patches in three equal zones along the long axis: red, tip with the larger number of patches; green, middle of the cell; blue, tip with the smaller number of patches. Darkly colored dots are the average proportion of patches in a given part of interphase cells. Lightly colored bars are ± 1 SD of these mean proportions. Lightly colored plus symbols (+) represent the distribution of patches in individual cells in cytokinesis. (D) Dispersion index OP50 (as defined in Berro and Pollard, 2014). Black dots are average values of the OP50 index in interphase cells. Gray bars are ± 1 SD of the means. Plus symbols are OP50 indices for individual cells in cytokinesis.
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Figure 6: Endocytic patch polarization and dispersion in wild-type and mutant cells. (A) Negative-contrast fluorescence micrographs of representative cells expressing Fim1p-mEGFP in seven strains indicated at the top of each set. First row, short interphase cells; middle row, medium-sized interphase cells; third row, cells in cytokinesis. (B) Graphs of the total number of patches per cell marked with Fim1p-mEGFP vs. the cell length for the strain indicated above each panel. Each symbol corresponds to an individual cell in interphase (●) or cytokinesis (+). Lines are the best linear correlations for the data (blue, y-intercept equals 0; black, free y-intercept). The equations for these lines are given at the bottom right of each graph. (C) Distributions of endocytic patches in three equal zones along the long axis: red, tip with the larger number of patches; green, middle of the cell; blue, tip with the smaller number of patches. Darkly colored dots are the average proportion of patches in a given part of interphase cells. Lightly colored bars are ± 1 SD of these mean proportions. Lightly colored plus symbols (+) represent the distribution of patches in individual cells in cytokinesis. (D) Dispersion index OP50 (as defined in Berro and Pollard, 2014). Black dots are average values of the OP50 index in interphase cells. Gray bars are ± 1 SD of the means. Plus symbols are OP50 indices for individual cells in cytokinesis.

Mentions: In wild-type cells, the intracellular distribution of endocytic patches varies predictably across the cell cycle (Figure 6A; Marks and Hyams, 1985; Berro and Pollard, 2014). Just after cell division, patches concentrate at the new tip, but later concentrate at both tips. During cytokinesis, patches concentrate around the middle of the cell flanking the contractile ring.


Synergies between Aip1p and capping protein subunits (Acp1p and Acp2p) in clathrin-mediated endocytosis and cell polarization in fission yeast.

Berro J, Pollard TD - Mol. Biol. Cell (2014)

Endocytic patch polarization and dispersion in wild-type and mutant cells. (A) Negative-contrast fluorescence micrographs of representative cells expressing Fim1p-mEGFP in seven strains indicated at the top of each set. First row, short interphase cells; middle row, medium-sized interphase cells; third row, cells in cytokinesis. (B) Graphs of the total number of patches per cell marked with Fim1p-mEGFP vs. the cell length for the strain indicated above each panel. Each symbol corresponds to an individual cell in interphase (●) or cytokinesis (+). Lines are the best linear correlations for the data (blue, y-intercept equals 0; black, free y-intercept). The equations for these lines are given at the bottom right of each graph. (C) Distributions of endocytic patches in three equal zones along the long axis: red, tip with the larger number of patches; green, middle of the cell; blue, tip with the smaller number of patches. Darkly colored dots are the average proportion of patches in a given part of interphase cells. Lightly colored bars are ± 1 SD of these mean proportions. Lightly colored plus symbols (+) represent the distribution of patches in individual cells in cytokinesis. (D) Dispersion index OP50 (as defined in Berro and Pollard, 2014). Black dots are average values of the OP50 index in interphase cells. Gray bars are ± 1 SD of the means. Plus symbols are OP50 indices for individual cells in cytokinesis.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 6: Endocytic patch polarization and dispersion in wild-type and mutant cells. (A) Negative-contrast fluorescence micrographs of representative cells expressing Fim1p-mEGFP in seven strains indicated at the top of each set. First row, short interphase cells; middle row, medium-sized interphase cells; third row, cells in cytokinesis. (B) Graphs of the total number of patches per cell marked with Fim1p-mEGFP vs. the cell length for the strain indicated above each panel. Each symbol corresponds to an individual cell in interphase (●) or cytokinesis (+). Lines are the best linear correlations for the data (blue, y-intercept equals 0; black, free y-intercept). The equations for these lines are given at the bottom right of each graph. (C) Distributions of endocytic patches in three equal zones along the long axis: red, tip with the larger number of patches; green, middle of the cell; blue, tip with the smaller number of patches. Darkly colored dots are the average proportion of patches in a given part of interphase cells. Lightly colored bars are ± 1 SD of these mean proportions. Lightly colored plus symbols (+) represent the distribution of patches in individual cells in cytokinesis. (D) Dispersion index OP50 (as defined in Berro and Pollard, 2014). Black dots are average values of the OP50 index in interphase cells. Gray bars are ± 1 SD of the means. Plus symbols are OP50 indices for individual cells in cytokinesis.
Mentions: In wild-type cells, the intracellular distribution of endocytic patches varies predictably across the cell cycle (Figure 6A; Marks and Hyams, 1985; Berro and Pollard, 2014). Just after cell division, patches concentrate at the new tip, but later concentrate at both tips. During cytokinesis, patches concentrate around the middle of the cell flanking the contractile ring.

Bottom Line: Capping protein and Aip1p help maintain the high density of actin filaments in meshwork by keeping actin filaments close enough for cross-linking.Our experiments also reveal new cellular functions for Acp1p and Acp2p independent of their capping activity.We identified two independent pathways that control polarization of endocytic sites, one depending on acp2(+) and aip1(+) during interphase and the other independent of acp1(+), acp2(+), and aip1(+) during mitosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103 Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8103 Nanobiology Institute, Yale University, New Haven, CT 06520-8103 Institut Camille Jordan, UMR CNRS 5208, Université de Lyon, 69622 Villeurbanne-Cedex, France Centre de Génétique et de Physiologie Moléculaire et Cellulaire, UMR CNRS 5534, Université de Lyon, 69622 Villeurbanne-Cedex, France.

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Related in: MedlinePlus