The Golgi ribbon structure facilitates anterograde transport of large cargoes.
Bottom Line: Yet the purpose of this remarkable structure has been an enigma.In addition, insect cells that naturally harbor dispersed Golgi stacks have limited capacity to transport artificial oversized cargoes.These results imply that the ribbon structure is an essential requirement for transport of large cargoes in mammalian cells, and we suggest that this is because it enables the dilated rims of cisternae (containing the aggregates) to move across the stack as they transfer among adjacent stacks within the ribbon structure.
Affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 firstname.lastname@example.org email@example.com.Show MeSH
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Mentions: One interpretation of the results reported above would be to consider that the phenotype is mainly due to the alteration of the microtubule network and is only an indirect consequence of the ribbon breakdown. To test this hypothesis, we decided to knock down grasp55/65, which is known to disrupt the ribbon structure of the Golgi without affecting the microtubule organization (Puthenveedu et al., 2006; Feinstein and Linstedt, 2008; Lee et al., 2014). As judged by immunoblotting and immunofluorescence analysis (Figure 4, A–C), at least 75% of the grasp55/65 proteins were efficiently silenced within cells treated with specific siRNA. As previously reported (Feinstein and Linstedt, 2008), we confirmed that these cells harbored a mildly scattered Golgi (Figure 4A and graph), without showing any alteration of the microtubules (Figure S3).
Affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520 firstname.lastname@example.org email@example.com.