Actin cable distribution and dynamics arising from cross-linking, motor pulling, and filament turnover.
Bottom Line: Our simulations reproduce the particular actin cable structures in myoVΔ cells and predict the effect of increased myosin V pulling.Increasing cross-linking parameters generates thicker actin cables.It also leads to antiparallel and parallel phases with straight or curved cables, consistent with observations of cells overexpressing α-actinin.
Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.Show MeSH
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Mentions: For3p generates cables from tip cortical sites containing ∼10 For3p dimers/site (Martin and Chang, 2006). This For3p clustering may help the generation of actin bundles and control of their thickness. It is also possible that For3p clustering is not required for cable formation, since filaments growing from For3p at distant tip sites can form bundles by cross-linking in the cytoplasm. We examined how actin cable distribution depends on the degree of clustering of For3p at cell tips and cross-linking among filaments. In Figure 7 we vary the number of cortical tip sites and distribute a fixed number of filament nuclei randomly among these sites. The cortical cluster sites are randomly distributed on the semispherical cell tip. Formins within the same cortical site are distributed randomly over a small area around the center of the site. We also study the interplay with cross-linking by varying the effective cross-linking range parameter rcrslnk.
Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.