Actin cable distribution and dynamics arising from cross-linking, motor pulling, and filament turnover.
Bottom Line: Our simulations reproduce the particular actin cable structures in myoVΔ cells and predict the effect of increased myosin V pulling.Increasing cross-linking parameters generates thicker actin cables.It also leads to antiparallel and parallel phases with straight or curved cables, consistent with observations of cells overexpressing α-actinin.
Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.Show MeSH
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Mentions: To further investigate the predicted effect of cross-linkers, we analyzed actin cables in wild-type cells and cells overexpressing α-actinin, using the 3nmt1Ain1 promoter (Figure 6A). We chose α-actinin because the effect of overexpression of the other cross-linker in fission yeast, fimbrin, is very drastic, with overexpression mutants having significantly modified cable morphologies (Wu et al., 2001; Laporte et al., 2012; Burke et al., 2014; see also Discussion). To better visualize actin cables, we also treated cells with CK666, an inhibitor of the Arp2/3 complex that depolymerizes actin patches (Nolen et al., 2009). This treatment causes an increase in the amount of actin in the cables, which also become longer and more curved (Burke et al., 2014). Treatment by CK666 may also release fimbrin from actin patches, resulting in an increase in cable cross-linking (Burke et al., 2014).
Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.