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Actin cable distribution and dynamics arising from cross-linking, motor pulling, and filament turnover.

Tang H, Laporte D, Vavylonis D - Mol. Biol. Cell (2014)

Bottom Line: Our simulations reproduce the particular actin cable structures in myoVΔ cells and predict the effect of increased myosin V pulling.Increasing cross-linking parameters generates thicker actin cables.It also leads to antiparallel and parallel phases with straight or curved cables, consistent with observations of cells overexpressing α-actinin.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.

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Related in: MedlinePlus

Simulated actin cables using parameter values from Table 1. (A) Time evolution of the simulation. Nucleus (yellow) and vacuoles/organelles (cyan) are simulated as impenetrable immobile spheres. The cytoplasmic region is marked in light green. Actin filaments growing from the left (right) side tip are marked in blue (red). Formin For3p clusters localized at the cell tip are marked in orange. (B) Simulated steady-state actin cables viewed from different perspectives (front, 45°, and 90°) showing a complete 3D cable structure.
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Figure 2: Simulated actin cables using parameter values from Table 1. (A) Time evolution of the simulation. Nucleus (yellow) and vacuoles/organelles (cyan) are simulated as impenetrable immobile spheres. The cytoplasmic region is marked in light green. Actin filaments growing from the left (right) side tip are marked in blue (red). Formin For3p clusters localized at the cell tip are marked in orange. (B) Simulated steady-state actin cables viewed from different perspectives (front, 45°, and 90°) showing a complete 3D cable structure.

Mentions: Simulation results using the parameter set in Table 1 reproduce actin cable structures similar to those observed in cells. Figure 2A shows simulations of actin filaments that grow out of 12 cortical sites per tip, with blue and red colors marking filaments that grow from either tip. After 180 s, which is long enough to reach steady state (see Materials and Methods), the filaments organize into a few bundles that contain filaments in both parallel and antiparallel orientation (Figure 2B). Filaments grow through the gaps among vacuoles and the nucleus, reaching filaments that polymerize from the opposite tip, generating bundles that span the cell length as in experimental images (Figure 1A).


Actin cable distribution and dynamics arising from cross-linking, motor pulling, and filament turnover.

Tang H, Laporte D, Vavylonis D - Mol. Biol. Cell (2014)

Simulated actin cables using parameter values from Table 1. (A) Time evolution of the simulation. Nucleus (yellow) and vacuoles/organelles (cyan) are simulated as impenetrable immobile spheres. The cytoplasmic region is marked in light green. Actin filaments growing from the left (right) side tip are marked in blue (red). Formin For3p clusters localized at the cell tip are marked in orange. (B) Simulated steady-state actin cables viewed from different perspectives (front, 45°, and 90°) showing a complete 3D cable structure.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4230589&req=5

Figure 2: Simulated actin cables using parameter values from Table 1. (A) Time evolution of the simulation. Nucleus (yellow) and vacuoles/organelles (cyan) are simulated as impenetrable immobile spheres. The cytoplasmic region is marked in light green. Actin filaments growing from the left (right) side tip are marked in blue (red). Formin For3p clusters localized at the cell tip are marked in orange. (B) Simulated steady-state actin cables viewed from different perspectives (front, 45°, and 90°) showing a complete 3D cable structure.
Mentions: Simulation results using the parameter set in Table 1 reproduce actin cable structures similar to those observed in cells. Figure 2A shows simulations of actin filaments that grow out of 12 cortical sites per tip, with blue and red colors marking filaments that grow from either tip. After 180 s, which is long enough to reach steady state (see Materials and Methods), the filaments organize into a few bundles that contain filaments in both parallel and antiparallel orientation (Figure 2B). Filaments grow through the gaps among vacuoles and the nucleus, reaching filaments that polymerize from the opposite tip, generating bundles that span the cell length as in experimental images (Figure 1A).

Bottom Line: Our simulations reproduce the particular actin cable structures in myoVΔ cells and predict the effect of increased myosin V pulling.Increasing cross-linking parameters generates thicker actin cables.It also leads to antiparallel and parallel phases with straight or curved cables, consistent with observations of cells overexpressing α-actinin.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.

Show MeSH
Related in: MedlinePlus