Actin cable distribution and dynamics arising from cross-linking, motor pulling, and filament turnover.
Bottom Line: Our simulations reproduce the particular actin cable structures in myoVΔ cells and predict the effect of increased myosin V pulling.Increasing cross-linking parameters generates thicker actin cables.It also leads to antiparallel and parallel phases with straight or curved cables, consistent with observations of cells overexpressing α-actinin.
Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.Show MeSH
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Mentions: Simulation results using the parameter set in Table 1 reproduce actin cable structures similar to those observed in cells. Figure 2A shows simulations of actin filaments that grow out of 12 cortical sites per tip, with blue and red colors marking filaments that grow from either tip. After 180 s, which is long enough to reach steady state (see Materials and Methods), the filaments organize into a few bundles that contain filaments in both parallel and antiparallel orientation (Figure 2B). Filaments grow through the gaps among vacuoles and the nucleus, reaching filaments that polymerize from the opposite tip, generating bundles that span the cell length as in experimental images (Figure 1A).
Affiliation: Department of Physics, Lehigh University, Bethlehem, PA 18015.