The Abl/enabled signaling pathway regulates Golgi architecture in Drosophila photoreceptor neurons.
Bottom Line: The Abl effector, Enabled (Ena), selectively labels the cis-Golgi in developing PRs.Finally, we demonstrate that the effects of Abl signaling on Golgi are mediated via regulation of the actin cytoskeleton.Moreover, they raise the possibility that some of the effects of Abl signaling may arise, in part, from alterations of protein trafficking and secretion.
Affiliation: Axon Guidance and Neural Connectivity Unit, Basic Neuroscience Program, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.Show MeSH
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Mentions: We found that the Abl antagonist Ena is associated with the cis-Golgi compartment in wild-type Drosophila photoreceptor (PR) neurons (Figure 1, A and C– E). In late third-instar eye imaginal disks, Ena is localized in three subcellular compartments in PR neuronal cell bodies. These are 1) actin-rich apical microvilli-like structures that at later stages develop into mature rhabdomeres; 2) the cortical actin cytoskeleton, which shows diffuse and uniform accumulation of Ena; and 3) distinct perinuclear flattened structures in the cytoplasm. We found strong colabeling of these Ena-enriched flattened structures with GM130, a bona fide marker for the cis-Golgi compartment of the Golgi complex (Figure 1A). Approximately 72.2% ± 1.0 (percent ± SEM) of Ena puncta were associated with GM130 cis-Golgi structures (n = 769 puncta from 11 wild-type disks). Ena structures do not show obvious overlap with other endomembrane compartments, such as early endosomes (Rab5-GFP), late endosomes and lysosomes (Rab11), or centrosomes (CNN–green fluorescent protein [GFP]; unpublished data).
Affiliation: Axon Guidance and Neural Connectivity Unit, Basic Neuroscience Program, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.